CAJ | 학술논문

以高功率发光二极管(LED)为激发光源,研制了一种小型LED诱导荧光检测器,用于微流控芯片分析检测.利用MOS管压控恒流原理,设计恒流驱动电路,使高功率LED发光稳定.通过设计和制造光学结构,成功地将LED发散光聚焦成约3.5 mm×0.3 mm的线状光束,实现了与微流控芯片中的微通道对准,简化了复杂的机械校准结构,且大大减少了光学系统体积(约为9.5 cm×4 cm×17 cm).用荧光染料NBD和高密度脂蛋白评价该体系的性能,结果表明,LED激发光源稳定,检测器重复性好,可用于微流控芯片毛细管电泳分析检测,基本实现了LED诱导荧光检测器的微型化.
이고공솔발광이겁관(LED)위격발광원,연제료일충소형LED유도형광검측기,용우미류공심편분석검측.이용MOS관압공항류원리,설계항류구동전로,사고공솔LED발광은정.통과설계화제조광학결구,성공지장LED발산광취초성약3.5 mm×0.3 mm적선상광속,실현료여미류공심편중적미통도대준,간화료복잡적궤계교준결구,차대대감소료광학계통체적(약위9.5 cm×4 cm×17 cm).용형광염료NBD화고밀도지단백평개해체계적성능,결과표명,LED격발광원은정,검측기중복성호,가용우미류공심편모세관전영분석검측,기본실현료LED유도형광검측기적미형화.
A novel miniaturized fluorescence detector by using a blue high-power Light-emitting-Diode(LED) as its excitation light source was developed for microfluidic chip analysis. Based on the constant current principle of MOS tubes,a constant current driving circuit was designed to control the LED to generate a stable light.A special optical structure was designed and manufacturized to focus the divergent light emitted by the LED into a linear beam with the size about 3.5 mm×0.3 mm. This linear beam could be easily aligned to the microchannel of a microfluidic chips to reduce the complexity of the detector and miniaturize the size of the detector, and the bulk of the optical system was about 9.5 cm×4 cm×17 cm. NBD C6-ceramide and high density lipoprotein were used to evaluate the performance of the detector, the results indicate that the LED excitation source is stable,and the detector has good reproducibility and can be used in the capillary electrophoresis analysis of microfluidic chips.