CAJ | 학술논문

采用根冠细胞测定法,以玉米小斑病菌C小种毒素(HMC-toxin)处理基因型为B37的同核异质体(Homocaryon)雄不育C细胞质和正常N细胞质玉米的根冠离体细胞后,采用吖啶橙荧光染色,荧光显微镜下发现HMC毒素可引起细胞凋亡,C细胞质中的凋亡细胞,多于N细胞质中的凋亡细胞,在对照中未观察到凋亡现象.还记载了玉米根冠细胞凋亡的具体过程.
채용근관세포측정법,이옥미소반병균C소충독소(HMC-toxin)처리기인형위B37적동핵이질체(Homocaryon)웅불육C세포질화정상N세포질옥미적근관리체세포후,채용아정등형광염색,형광현미경하발현HMC독소가인기세포조망,C세포질중적조망세포,다우N세포질중적조망세포,재대조중미관찰도조망현상.환기재료옥미근관세포조망적구체과정.
Apoptosis in detached corn root cap cells under fluorescent microscope was studied. A pair of homokaryon(CB37 and B37)inbred lines in maize was tested and the detached corn root cap cells might be using acridine orange staining. Apoptosis was found under fluorescence microscope treated by fihrated culture fluid of HMC toxin. The sensitivity of HMC toxin to C cytoplasm was greater than N cytoplasm,so that the quantity of the apoptosis was great more in the detached corn root cap cells with C cytoplasm than with N cytoplasm. Besides, apoptosis was not found in the CK. These indicated that special disease response occurred in the course of apoptosis in detached corn root cap cells of C cytoplasm maize led by HMC.