CAJ | 학술논문

目的对细菌纤维素高产菌株葡糖醋杆菌G-29进行发酵优化,以提高其细菌纤维素合成能力.方法采用基于非完全平衡块原理的Plackett-Burman(PB) 试验设计法,筛选出了3个主要影响因子:混合碳源(葡萄糖:蔗糖=1:2),MgSO_4,乙醇,然后采用Box-Behnten中心,组合试验设计和响应面分析方法(RSM)确定其最佳浓度分别为混合碳原52.3g/L,MgSO_40.159 8 g/L,乙醇32.5 ml/L.用扫描电镜观察了细菌纤维素的形态结构,并通过X射线衍射分析其在干态下和湿态下的最佳结构.结果在优化培养基条件下,菌株G-29发酵生产细菌纤维素的产量为11.83 g/L,是优化前产量(6.20 g/L)的1.9倍.结论 PB试验和RSM相结合的试验方法,用于优化G-29发酵培养基,不仅科学合理而且准确有效.
목적 대세균섬유소고산균주포당작간균G-29진행발효우화,이제고기세균섬유소합성능력.방법 채용기우비완전평형괴원리적Plackett-Burman(PB) 시험설계법,사선출료3개주요영향인자:혼합탄원(포도당:자당=1:2),MgSO_4,을순,연후채용Box-Behnten중심,조합시험설계화향응면분석방법(RSM)학정기최가농도분별위혼합탄원52.3g/L,MgSO_40.159 8 g/L,을순32.5 ml/L.용소묘전경관찰료세균섬유소적형태결구,병통과X사선연사분석기재간태하화습태하적최가결구.결과 재우화배양기조건하,균주G-29발효생산세균섬유소적산량위11.83 g/L,시우화전산량(6.20 g/L)적1.9배.결론 PB시험화RSM상결합적시험방법,용우우화G-29발효배양기,불부과학합리이차준학유효.
Objective In this manuscript,the optimal conditions for cellulose production by gluconacetoba-ater G-29 were determined in order to improve the yield of bacterial cellulose.Methods Plackett-Burman Design was used to evaluate three statistically significant parameters including mixed carbon sources(Glucose:sucrose=1:2),MgSO_4,ethanol.The optimal levels of three variables were defined by Box-Behnken design and response surface method(RSM):mixed carbon sources:52.3 g/L,MgSO_4 0.159 g/L,ethanol 32.5 g/L,ethanol 32.5 g/L comparison.The morphology of bacterial cellulose(BC) produced was observed by scanning electron microscope(SEM).The crystal structure of BC in dried and wet state was studied by X-ray diffractometry(XRD).Results The results indicated that the BC yield under the optimal fermentation medium was 11.83 g/L,which was as 1.9 times as that under the original fermentation medium.Conclusion The method of PB design combined with RSM used for optimization of G-29 fermentation medium is scientific and accurate.