CAJ | 학술논문

报道天然彩色棉纤维特异表达启动子LTP3,大小为1 548 bp。经测序分析,与GenBank中登录的海岛棉（Gossypium hirutum L.DES119）特异表达启动子序列同源性为99%,仅有个别碱基发生改变,而启动子的基本元件未发生突变。将克隆的启动子与报告基因GUS融合,构建植物表达载体pBI-LTP3,并转化烟草。结果表明GUS基因仅在转基因烟草叶片表皮毛中表达。
보도천연채색면섬유특이표체계동자LTP3,대소위1 548 bp。경측서분석,여GenBank중등록적해도면（Gossypium hirutum L.DES119）특이표체계동자서렬동원성위99%,부유개별감기발생개변,이계동자적기본원건미발생돌변。장극륭적계동자여보고기인GUS융합,구건식물표체재체pBI-LTP3,병전화연초。결과표명GUS기인부재전기인연초협편표피모중표체。
PCR were performed to clone LTP3 promoter from color cotton.Sequencing analysis results indicated that it contained 1 548 nt;the homology is 99% compared with the LTP3 reported by Liu,and only 4 nucleotides were changed.The LTP3（1548bp） promoter was fused with the E.coli.β-glucuronidase gene（GUS） and transformed into tobacco.The results suggested that GUS expressions under control of LTP3 promoter were detected only in the trichomes of transgenic tobacco leaves,while the positive control,contains Cauliflower mosaic Virus（CaMV） 35S promoter and GUS gene,was constitutively expressed,and there are no GUS expression in the negative control（untransformation plants）.The results indicated that LTP3 promoter can be used to express the heterologous genes in tobacco leaves.