CAJ | 학술논문

目的分析甲状腺乳头状癌(PTC)肿瘤微环境T淋巴细胞各亚群的相对分布.方法应用流式细胞术测定35例PTC患者、25例良性甲状腺病变的局部肿瘤组织以及15例健康体检者外周血CD3+ CD4+ CD8-、CD3+ CD4- CD8+、CD3+CD4+CD8+、CD3+ CD4- CD8- 各T细胞亚群的水平及CD4+/CD8+比值.结果外周血、良性组和PTC肿瘤微环境的CD4+/CD8+比值明显依次递减(P<0.01);PTC和良性组局部组织间的CD3+ CD4+ CD8+细胞水平显著高于外周血(P<0.01).但前二者间的CD3+ CD4+ CD8+细胞水平差异无统计学意义(P>0.05);良性组的CD3+CD4-CD8-细胞水平显著高于PTC局部和外周血(P<0.01),后二者间的CD3+CD4-CD8-细胞水平的差异无统计学意义(P>0.05);15例伴淋巴结转移PTC局部癌组织的CD4+/CD8+比值及CD3+ CD4+ CD8-、CD3+ CD4-CD8+、CD3+CD4+CD8+、CD3+CD4-CD8-各T细胞亚群的水平与20例无淋巴结转移的PTC局部癌组织之间的差异无统计学意义(P>0.05).结论 PTC肿瘤微环境免疫细胞以CD8+阳性T细胞为主.局部呈明显的免疫受抑状态,而甲状腺良性病变局部CD3+CD4+CD8+、CD3+CD4-CD8-T细胞的显著增多可能有利于营造抗瘤免疫效应的微环境,PTC肿瘤微环境中T淋巴细胞各亚群的分布与淋巴结癌转移与否无关.
목적 분석갑상선유두상암(PTC)종류미배경T림파세포각아군적상대분포.방법 응용류식세포술측정35례PTC환자、25례량성갑상선병변적국부종류조직이급15례건강체검자외주혈CD3+ CD4+ CD8-、CD3+ CD4- CD8+、CD3+CD4+CD8+、CD3+ CD4- CD8- 각T세포아군적수평급CD4+/CD8+비치.결과 외주혈、량성조화PTC종류미배경적CD4+/CD8+비치명현의차체감(P<0.01);PTC화량성조국부조직간적CD3+ CD4+ CD8+세포수평현저고우외주혈(P<0.01).단전이자간적CD3+ CD4+ CD8+세포수평차이무통계학의의(P>0.05);량성조적CD3+CD4-CD8-세포수평현저고우PTC국부화외주혈(P<0.01),후이자간적CD3+CD4-CD8-세포수평적차이무통계학의의(P>0.05);15례반림파결전이PTC국부암조직적CD4+/CD8+비치급CD3+ CD4+ CD8-、CD3+ CD4-CD8+、CD3+CD4+CD8+、CD3+CD4-CD8-각T세포아군적수평여20례무림파결전이적PTC국부암조직지간적차이무통계학의의(P>0.05).결론 PTC종류미배경면역세포이CD8+양성T세포위주.국부정명현적면역수억상태,이갑상선량성병변국부CD3+CD4+CD8+、CD3+CD4-CD8-T세포적현저증다가능유리우영조항류면역효응적미배경,PTC종류미배경중T림파세포각아군적분포여림파결암전이여부무관.
Objective To analyze the distribution of T lymphocyte subsets in tumor microenvironment of papillary thyroid carcinoma (PTC). Methods The levels of CD3+ CD4+ CD8- ,CD3+ CD4- CD8+ ,CD3+ CD4+ CD8+ ,CD3+ CD4- CD8- T cell and CD4+/CD8+ ra- tio in tumor microenvironment of 35 cases PTC, 25 cases benign thyroid lesion and 15 healthy eases were detected by flow cytometry. Results Local tumor tissue of 35 cases PTC and 25 cases benign thyroid lesion, and blood of 15 healthy cases were collected. The ratio of CD4+/ CD8+ in blood, tumor microenvironment of benign thyroid lesions and PTCs decreased significantly ( P<0.01). The levels of CD3+ CD4+ CD8+ cell in tumor microenvironment of PTC and benign thyroid lesion were higher than that in peripheral blood( P<0.01). But the differ- ence of the level of CD3+ CD4+ CD8+ cell between PTC and benign thyroid lesion was not significant ( P>0.05). The levels of CD3+ CD4- CD8- cell in tumor microenvironment of benign thyroid lesion were higher than those in PTC and peripheral blood(P<0.01). The difference of the level of CD3+ CD4- CD8- cell between PTC and peripheral blood was not significant (P>0.05 ). The differences of CD4+/CD8+ ratio and the level of each of T lymphocyte subset such as CD3+ CD4+ CD8-, CD3+ CD4- CD8+, CD3+ CD4+ CD8+, CD3+ CD4- CD8- cell between 15 PTC cases with lymph node metastasis and 20 PTC cases without metastasis were not significant (P>0.05). Conclusions CD8+ T cell is the main immune cell in tumor microenvironment in PTC. The local cell-mediated immunity of PTC is in the status of obviously suppressed. But the obvious increase of CD3+ CD4+ CD8+ and CD3+ CD4- CD8- T cells in tumor microenvironment in thyroid benign lesion may be beneficial for creating the location for immune responding against tumor. The distribution of T lymphocyte sub- sets in tumor microenvironment in PTC is not associated with lymph node metastasis.