CAJ | 학술논문

目的建立丙型肝炎病毒总核心抗原(总HCV-cAg)酶联免疫测定方法,并对相关的临床样品进行测定.方法通过对样品进行裂解处理,采用酶联免疫试剂盒(ELISA)检测总HCV-cAg,对201名抗-HCV阳性者血清进行总HCV-cAg检测,同时进一步作HCV-RNA检测,其中176例采用荧光定量PCR(FQ-PCR),25例采用逆转录PCR(RT-PCR)检测HCV-RNA.结果共检测201份血清标本,其中经PCR测定HCV RNA阳性88人份,阳性率43.8%;总HCV-cAg阳性71份,阳性率35.3%.经统计学分析,总HCV-cAg检测和HCV RNA检测的阳性率的差异无统计学意义.结论建立的总HCV-cAg酶联免疫测定方法适合临床应用,尤其适合在缺少RT-PCR或荧光定量PCR的中小医院使用.
목적 건립병형간염병독총핵심항원(총HCV-cAg)매련면역측정방법,병대상관적림상양품진행측정.방법 통과대양품진행렬해처리,채용매련면역시제합(ELISA)검측총HCV-cAg,대201명항-HCV양성자혈청진행총HCV-cAg검측,동시진일보작HCV-RNA검측,기중176례채용형광정량PCR(FQ-PCR),25례채용역전록PCR(RT-PCR)검측HCV-RNA.결과 공검측201빈혈청표본,기중경PCR측정HCV RNA양성88인빈,양성솔43.8%;총HCV-cAg양성71빈,양성솔35.3%.경통계학분석,총HCV-cAg검측화HCV RNA검측적양성솔적차이무통계학의의.결론 건립적총HCV-cAg매련면역측정방법괄합림상응용,우기괄합재결소RT-PCR혹형광정량PCR적중소의원사용.
Objective To develop the technique to detect total core antigen of HCV(Total HCV-cAg) by Enzyme-Linked Immu-nosorbent Assay (ELISA) and apply it for clinical diagnosis. Methods 201 serum samples with anti-HCV antibody were detected total HCV-cAg after pre - treating the samples, then the sensitivity of results were compared with HCV RNA tests. Among them, 176 cases was determined by FQ-PCR, and 25 cases by RT-PCR for HCV-RNA. Results HCV RNA was found in sera from 88 of 201 samples (43.8%). Total HCV-cAg was positive in 71 (35.3%) of 201 samples . There was no significant difference between the detection rate of HCV RNA by PCR and total HCV-cAg by ELISA. Conclusion Detection of total core antigen of HCV is suitable to be used as to diagnose HCV in clinic.