中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2008年
6期
397-400,插3
,共5页
顾志峰%赵盛楠%张华勇%徐婷%金鸥阳%周康兴%刘海鸥%张天一%孙凌云
顧誌峰%趙盛楠%張華勇%徐婷%金鷗暘%週康興%劉海鷗%張天一%孫凌雲
고지봉%조성남%장화용%서정%금구양%주강흥%류해구%장천일%손릉운
红斑狼疮,系统性%骨髓间后干细胞%超微结构%肌动蛋白%纽蛋白
紅斑狼瘡,繫統性%骨髓間後榦細胞%超微結構%肌動蛋白%紐蛋白
홍반랑창,계통성%골수간후간세포%초미결구%기동단백%뉴단백
Lupus erythematosus,systemic%Mesenchymal stem cells%Ultrastructure%Actin%Vinculin
目的 探讨系统性红斑狼疮(SLE)患者骨髓间质干细胞(MSCs)的超微结构及细胞骨架的形态学特征.方法 体外培养SLE患者的MSCs后,透射电子显微镜(TEM)、激光共聚焦显微镜(LCM)观察超微结构及肌动蛋白、纽蛋白的表达.结果 SLE MSCs透射电子显微镜下多数细胞内呈衰老征象,可见大量自噬体;细胞边缘应力纤维增多,排列紊乱,组成粗大的强荧光团,核周围应力纤维减少;纽蛋白在细胞边缘排列紊乱,细胞核周围增多.结论 SLE MSCs超微结构及细胞骨架的改变是细胞扩增不良的原因之一.
目的 探討繫統性紅斑狼瘡(SLE)患者骨髓間質榦細胞(MSCs)的超微結構及細胞骨架的形態學特徵.方法 體外培養SLE患者的MSCs後,透射電子顯微鏡(TEM)、激光共聚焦顯微鏡(LCM)觀察超微結構及肌動蛋白、紐蛋白的錶達.結果 SLE MSCs透射電子顯微鏡下多數細胞內呈衰老徵象,可見大量自噬體;細胞邊緣應力纖維增多,排列紊亂,組成粗大的彊熒光糰,覈週圍應力纖維減少;紐蛋白在細胞邊緣排列紊亂,細胞覈週圍增多.結論 SLE MSCs超微結構及細胞骨架的改變是細胞擴增不良的原因之一.
목적 탐토계통성홍반랑창(SLE)환자골수간질간세포(MSCs)적초미결구급세포골가적형태학특정.방법 체외배양SLE환자적MSCs후,투사전자현미경(TEM)、격광공취초현미경(LCM)관찰초미결구급기동단백、뉴단백적표체.결과 SLE MSCs투사전자현미경하다수세포내정쇠로정상,가견대량자서체;세포변연응력섬유증다,배렬문란,조성조대적강형광단,핵주위응력섬유감소;뉴단백재세포변연배렬문란,세포핵주위증다.결론 SLE MSCs초미결구급세포골가적개변시세포확증불량적원인지일.
Objective To explore ultrastructure and cytoskeleton characteristics of bone marrow-deftved mesenchymal stem cells (MSCs) in patients with systemic lupus erythematosus (SLE).Methods MSCs were isolated from bone marrow of 2 SLE patients and 2 healthy controls.Their ultrastrnctures were examined by transmission electron microscope (TEM).The expression pattern of actin and vinculin was assessed by laser confocal microscopy (LCM).Results MSCs in patients with SLE presented with signs of ageing and lots of autophagosome could be found in most of the cells.F-actin was aggregated and condensed at the:border of cytoplasm.Vinculin was arranged disorderly and condensed in the cytoplasm.Conclusion The change of uhrastructure and cytoskeleton patterns of bone marrow derived mesenchymal cells of SLE patients may play an important role in the abnormal proliferation of these cells in vitro.
