国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2011年
12期
903-906,封3
,共5页
赖添顺%阮世冲%蔡绍曦%赵海金%郭振辉%刘来昱
賴添順%阮世遲%蔡紹晞%趙海金%郭振輝%劉來昱
뢰첨순%원세충%채소희%조해금%곽진휘%류래욱
呼吸机所致肺损伤%内皮素-1%机械通气%潮气量%急性肺损伤
呼吸機所緻肺損傷%內皮素-1%機械通氣%潮氣量%急性肺損傷
호흡궤소치폐손상%내피소-1%궤계통기%조기량%급성폐손상
Ventilator-induced lung injury%Endothelin-1%Mechanical ventilation%Tidal volume%Acute lung injury
目的 检测内皮素-1(ET-1)在呼吸机所致肺损伤(VILI)模型肺组织的表达分布及变化.方法 普通级犬随机分为正常对照组(N组,n=6)、急性肺损伤(ALI)组(n=14).用油酸静脉内注射制备ALI模型,制备成功后随机取2只作为ALI组,其他随机分为小潮气量机械通气组(LV组,n=6)、大潮气量机械通气组(即VILI组,n=6),行不同潮气量机械通气6 h(LV组潮气量6 ml/kg,VILI组潮气量20 ml/kg,呼气末正压=10 cm H2O).用HE染色病理观察各组病理变化并用Smith评分法进行肺损伤评分.用免疫组织化学法观察各组犬肺组织ET-1的分布,并比较各组ET-1变化.结果 LV组及VILI组出现肺泡隔增宽、炎性细胞浸润、肺泡出血、渗出和坏死等肺组织病理学改变,肺组织病理损伤评分LV组显著低于VILI组(P<0.01).免疫组织化学结果显示:N组血管内皮细胞、肺泡有少量ET-1表达;LV组和VILI组血管内皮细胞、肺泡ET-1的表达较N组显著增高(P值均<0.01),且VILI组血管内皮细胞及肺组织ET-1表达较LV组显著增高(P<0.01).结论 在VILI模型肺部血管上皮细胞及肺组织均存在ET-1的异常高表达,提示ET-1可能参与VILI的病理生理过程.
目的 檢測內皮素-1(ET-1)在呼吸機所緻肺損傷(VILI)模型肺組織的錶達分佈及變化.方法 普通級犬隨機分為正常對照組(N組,n=6)、急性肺損傷(ALI)組(n=14).用油痠靜脈內註射製備ALI模型,製備成功後隨機取2隻作為ALI組,其他隨機分為小潮氣量機械通氣組(LV組,n=6)、大潮氣量機械通氣組(即VILI組,n=6),行不同潮氣量機械通氣6 h(LV組潮氣量6 ml/kg,VILI組潮氣量20 ml/kg,呼氣末正壓=10 cm H2O).用HE染色病理觀察各組病理變化併用Smith評分法進行肺損傷評分.用免疫組織化學法觀察各組犬肺組織ET-1的分佈,併比較各組ET-1變化.結果 LV組及VILI組齣現肺泡隔增寬、炎性細胞浸潤、肺泡齣血、滲齣和壞死等肺組織病理學改變,肺組織病理損傷評分LV組顯著低于VILI組(P<0.01).免疫組織化學結果顯示:N組血管內皮細胞、肺泡有少量ET-1錶達;LV組和VILI組血管內皮細胞、肺泡ET-1的錶達較N組顯著增高(P值均<0.01),且VILI組血管內皮細胞及肺組織ET-1錶達較LV組顯著增高(P<0.01).結論 在VILI模型肺部血管上皮細胞及肺組織均存在ET-1的異常高錶達,提示ET-1可能參與VILI的病理生理過程.
목적 검측내피소-1(ET-1)재호흡궤소치폐손상(VILI)모형폐조직적표체분포급변화.방법 보통급견수궤분위정상대조조(N조,n=6)、급성폐손상(ALI)조(n=14).용유산정맥내주사제비ALI모형,제비성공후수궤취2지작위ALI조,기타수궤분위소조기량궤계통기조(LV조,n=6)、대조기량궤계통기조(즉VILI조,n=6),행불동조기량궤계통기6 h(LV조조기량6 ml/kg,VILI조조기량20 ml/kg,호기말정압=10 cm H2O).용HE염색병리관찰각조병리변화병용Smith평분법진행폐손상평분.용면역조직화학법관찰각조견폐조직ET-1적분포,병비교각조ET-1변화.결과 LV조급VILI조출현폐포격증관、염성세포침윤、폐포출혈、삼출화배사등폐조직병이학개변,폐조직병리손상평분LV조현저저우VILI조(P<0.01).면역조직화학결과현시:N조혈관내피세포、폐포유소량ET-1표체;LV조화VILI조혈관내피세포、폐포ET-1적표체교N조현저증고(P치균<0.01),차VILI조혈관내피세포급폐조직ET-1표체교LV조현저증고(P<0.01).결론 재VILI모형폐부혈관상피세포급폐조직균존재ET-1적이상고표체,제시ET-1가능삼여VILI적병리생리과정.
Objective To determine the change of endothelin-1 (ET-1) expression in the lung tissue of ventilator-induced lung injury (VILI) model. Methods The dogs were randomly divided into control group (N group, n =6) and acute lung injury (ALI) group ( n =14). ALI model was established by intravenously injecting with oleic acid. Thereafter two animals were obtained as ALI group, and the others were randomly divided into low tidal ventilation group (LV group, n =6) and high tidal ventilation group (i. e. VILI group, n =6). The tidal volume was respectively 6 ml/kg and 20 ml/kg in LV group and VILI group, and positive end expiratory pressure was 10 cm H2O. Lung tissues were stained with HE,the pathological changes of lung injury were evaluated with Smith scoring, the distribution of ET-1 was observed with immunohistochemistry technique, and changes of pathology and ET-1 expression in different groups were compared. Results There were widened alveolar septum, inflammatory cell infiltration,alveolar hemorrhage, exudation, and necrosis in LV group and VILI group. The lung injury score in LV group was lower than that in VILI group ( P <C0. 01). The immunohistochemistry results showed that small amount of ET-1 was expressed in pulmonary vascular endothelial cells and alveoli of N group,et-1 expression in pulmonary vascular endothelial cells and alveoli of LV group and VILI group was significantly higher than that in N group (all P < 0. 01), and ET-1 expression in VILI group was significantly higher than that in LV group ( P <0. 01). Conclusions ET-1 is overexpressed in pulmonary vascular endothelial cells and lung tissues of VILI model, which indicates that ET-1 may be involved in the pathophsiologic process of VILI.
