中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2009年
1期
41-43
,共3页
郑辉%涂序珉%甄荣%张志兴
鄭輝%塗序珉%甄榮%張誌興
정휘%도서민%견영%장지흥
累积照射%一氧化氮%总抗氧化能力%超氧化物歧化酶%谷光甘肽过氧化物酶%过氧化氢酶%丙二醛
纍積照射%一氧化氮%總抗氧化能力%超氧化物歧化酶%穀光甘肽過氧化物酶%過氧化氫酶%丙二醛
루적조사%일양화담%총항양화능력%초양화물기화매%곡광감태과양화물매%과양화경매%병이철
Accumulative irradiation%Nitric oxide%Total ability of anti-oxidize antioxidase%Superoxide dismutase%Glutathion peroxidase%Catalase%Maleic dialdehyde
目的 比较单次照射和累积剂量照射对大鼠血清一氧化氮(NO)、总抗氧化能力、抗氧化酶和丙二醛(MDA)含量改变.方法 48只SD大鼠随机分为单次照射组和累积剂量照射组.采用60CO γ线诱发单次和累积剂量照射模型.总照射剂量分别为0.4、0.8、1.6、3.2、6.4 Gy.末次照射后1 d检测大鼠血清NO,总抗氧化能力、超氧化物歧化酶(SOD)、谷光甘肽过氧化物酶(GSH-PX)、过氧化氢酶(CAT)活性和MDA含量.结果 累积照射后血清NO含量(52.6～117.9 μmol/ml),总抗氧化能力(3.3～26.2 U/ml)、SOD(26.3～167.5 U/ml)、GSH-PX(740.8～2462.4 U/ml)、CAT活性(3.3～29.4 U/ml)和MDA含量(29.3～155.1 nmol/ml)与单次照射组比较均有不同程度的增加,其变化幅度与累积受照总剂量有关.其中累积照射0.4 Gy组总抗氧化能力(26.2 U/ml)、SOD(167.5 U/ml)和CAT活性(29.4 U/ml)明显大于健康对照组水平,累积受照总剂量>3.2 Gy组血清MDA含量明显高于单次照射组,可能与血清中抗氧化酶活性较高有关.结论 累积低剂量电离辐射可诱发生物氧化酶的刺激或兴奋效应,累积高剂量电离辐射对机体抗氧化酶活性仍有明显的损伤作用.
目的 比較單次照射和纍積劑量照射對大鼠血清一氧化氮(NO)、總抗氧化能力、抗氧化酶和丙二醛(MDA)含量改變.方法 48隻SD大鼠隨機分為單次照射組和纍積劑量照射組.採用60CO γ線誘髮單次和纍積劑量照射模型.總照射劑量分彆為0.4、0.8、1.6、3.2、6.4 Gy.末次照射後1 d檢測大鼠血清NO,總抗氧化能力、超氧化物歧化酶(SOD)、穀光甘肽過氧化物酶(GSH-PX)、過氧化氫酶(CAT)活性和MDA含量.結果 纍積照射後血清NO含量(52.6～117.9 μmol/ml),總抗氧化能力(3.3～26.2 U/ml)、SOD(26.3～167.5 U/ml)、GSH-PX(740.8～2462.4 U/ml)、CAT活性(3.3～29.4 U/ml)和MDA含量(29.3～155.1 nmol/ml)與單次照射組比較均有不同程度的增加,其變化幅度與纍積受照總劑量有關.其中纍積照射0.4 Gy組總抗氧化能力(26.2 U/ml)、SOD(167.5 U/ml)和CAT活性(29.4 U/ml)明顯大于健康對照組水平,纍積受照總劑量>3.2 Gy組血清MDA含量明顯高于單次照射組,可能與血清中抗氧化酶活性較高有關.結論 纍積低劑量電離輻射可誘髮生物氧化酶的刺激或興奮效應,纍積高劑量電離輻射對機體抗氧化酶活性仍有明顯的損傷作用.
목적 비교단차조사화루적제량조사대대서혈청일양화담(NO)、총항양화능력、항양화매화병이철(MDA)함량개변.방법 48지SD대서수궤분위단차조사조화루적제량조사조.채용60CO γ선유발단차화루적제량조사모형.총조사제량분별위0.4、0.8、1.6、3.2、6.4 Gy.말차조사후1 d검측대서혈청NO,총항양화능력、초양화물기화매(SOD)、곡광감태과양화물매(GSH-PX)、과양화경매(CAT)활성화MDA함량.결과 루적조사후혈청NO함량(52.6～117.9 μmol/ml),총항양화능력(3.3～26.2 U/ml)、SOD(26.3～167.5 U/ml)、GSH-PX(740.8～2462.4 U/ml)、CAT활성(3.3～29.4 U/ml)화MDA함량(29.3～155.1 nmol/ml)여단차조사조비교균유불동정도적증가,기변화폭도여루적수조총제량유관.기중루적조사0.4 Gy조총항양화능력(26.2 U/ml)、SOD(167.5 U/ml)화CAT활성(29.4 U/ml)명현대우건강대조조수평,루적수조총제량>3.2 Gy조혈청MDA함량명현고우단차조사조,가능여혈청중항양화매활성교고유관.결론 루적저제량전리복사가유발생물양화매적자격혹흥강효응,루적고제량전리복사대궤체항양화매활성잉유명현적손상작용.
Objective To compare the changes in nitric oxide (NO), total ability of anti-oxidize antioxidase, superoxide dismutase (SOD), glutathion peroxidase (GSH-PX), catalase (CAT) and maleic dialdehyde(MDA) in rats after single and accumulative 60Co γ-irradiation . Methods 48 rats were randomly divided into two groups of single and accumulative irradiation. Each group was irradiated by 60Co gamma-rays. The total irradiation doses were 0.4, 0.8, 1.6, 3.2 and 6.4 Gy, respectively in each group. The NO, total ability of anti-oxidize antioxidaze, SOD, GSH-PX, CAT and MDA in serum were measured at day 1 after last irradiation. Results Compared with the single irradiation group, the NO (52.6-117.9 μmol/ml), total ability of anti-oxidize antioxidaze (3.3-26.2 U/ml), the antioxidase activity of the SOD (26.3-167.5 U/ml), GSH-PX (740.8-2462.4 U/ml), CAT (3.3-29.4 U/ml) and the content of MDA(29.3-155.1 nmol/ml) of mt serum in accumulative irradiation group were increased after irradiation, which was related with the accumulative irradiation dose. For instance, total ability of anti-oxidize antioxidase (26.2 U/ml), antioxidase activity of the SOD (167.5 U/ml) and CAT (29.4 U/ml) in 0.4 Gy group of accumulative irradiation were significandy increased when compared with those of control group. However, the content of MDA in accumulative irradiation group was obviously higher than that in single radiation group when the irradiation doses delivered over 3.2 Gy, which might be correlated with higher antioxidase activity. Conclusions Low dose of accumulative gamma-rays irradiation can induce the stimulative effect of antioxidase activity. However, higher dose of accumulative gamma-rays irradiation can damage the activity of antioxidase.
