中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2009年
2期
136-138
,共3页
蔡清颜%施亚雄%李永加%陶畊
蔡清顏%施亞雄%李永加%陶畊
채청안%시아웅%리영가%도경
脂联素%多态性%限制性片段长度%糖尿病,2型%颈动脉%血管内膜中层厚度
脂聯素%多態性%限製性片段長度%糖尿病,2型%頸動脈%血管內膜中層厚度
지련소%다태성%한제성편단장도%당뇨병,2형%경동맥%혈관내막중층후도
Adiponectin%Polymorphism,restriction fragment length%Diabetes mellitus,type 2%Carotid artery%Intima-media thickness
目的 探讨脂联素基因启动子区-11377位点单核苷酸多态性与2型糖尿病患者颈动脉内膜中层厚度(CIMT)之间的关系.方法 采用PCR-限制性片段长度多态性(RFLP)技术在504例2型糖尿病患者(CIMT正常组254例,CIMT增厚组250例)中检测脂联素基因-11377位点多态性,同时检测血脂、空腹血糖、空腹胰岛素及血清脂联素水平.结果 脂联素基因-11377C→G基因型和等位基因频率在CIMT正常组与CIMT增厚组的分布有显著性差别(P<O.01);-11377CC基因型组CIMT值、低密度脂蛋白胆固醇、舒张压、稳态模型评估的胰岛素抵抗指数(HOMA-IR)低于CG+GG组(P<O.05或P<O.01),而血清脂联素水平高于CG+GG组[(5.70±3.58 vs 4.72±2.34)mg/L,P<0.01].脂联素基因启动子-11377C→G变异与2型糖尿病患者CIMT相关,且独市于脂联素水平(P=O.037).结论 福建地区2型糖尿病患者脂联素基因-11377c→G多态性与CIMT相关.
目的 探討脂聯素基因啟動子區-11377位點單覈苷痠多態性與2型糖尿病患者頸動脈內膜中層厚度(CIMT)之間的關繫.方法 採用PCR-限製性片段長度多態性(RFLP)技術在504例2型糖尿病患者(CIMT正常組254例,CIMT增厚組250例)中檢測脂聯素基因-11377位點多態性,同時檢測血脂、空腹血糖、空腹胰島素及血清脂聯素水平.結果 脂聯素基因-11377C→G基因型和等位基因頻率在CIMT正常組與CIMT增厚組的分佈有顯著性差彆(P<O.01);-11377CC基因型組CIMT值、低密度脂蛋白膽固醇、舒張壓、穩態模型評估的胰島素牴抗指數(HOMA-IR)低于CG+GG組(P<O.05或P<O.01),而血清脂聯素水平高于CG+GG組[(5.70±3.58 vs 4.72±2.34)mg/L,P<0.01].脂聯素基因啟動子-11377C→G變異與2型糖尿病患者CIMT相關,且獨市于脂聯素水平(P=O.037).結論 福建地區2型糖尿病患者脂聯素基因-11377c→G多態性與CIMT相關.
목적 탐토지련소기인계동자구-11377위점단핵감산다태성여2형당뇨병환자경동맥내막중층후도(CIMT)지간적관계.방법 채용PCR-한제성편단장도다태성(RFLP)기술재504례2형당뇨병환자(CIMT정상조254례,CIMT증후조250례)중검측지련소기인-11377위점다태성,동시검측혈지、공복혈당、공복이도소급혈청지련소수평.결과 지련소기인-11377C→G기인형화등위기인빈솔재CIMT정상조여CIMT증후조적분포유현저성차별(P<O.01);-11377CC기인형조CIMT치、저밀도지단백담고순、서장압、은태모형평고적이도소저항지수(HOMA-IR)저우CG+GG조(P<O.05혹P<O.01),이혈청지련소수평고우CG+GG조[(5.70±3.58 vs 4.72±2.34)mg/L,P<0.01].지련소기인계동자-11377C→G변이여2형당뇨병환자CIMT상관,차독시우지련소수평(P=O.037).결론 복건지구2형당뇨병환자지련소기인-11377c→G다태성여CIMT상관.
Objective To explore the association of-11377 site single nucleotide polymorphism in promoter region of adiponectin gene and carotid intima-media thickness(CIMT)in patients with type 2 diabetes mellitus.Methods The adiponectin gene-11377C→G polymorphism was identified by PCR-restriction fragment length polymorphism(RFLP)in 504 patients with type 2 diabetes mellitus(250 patients with increased CIMT and254 Datients with normal CIMT). Serum lipid and fasting plasma glucose were detected by full automatic biochemical analysor.fasting serum insulin(FINS) was measured by radioimmunoassay,and serum adiponectin level was assessed by ELISA.Results The frequencies of adiponectin gene-11377C→G genotype and allele were different between type 2 diabetic patients with normal and increased CIMT(P<O.01).In type 2 diabetic patients, the values of CIMT,diastolic blood pressure,low-density lipoprotein cholesterol (LDL-C)and homeostasis model assessment of insulin resistance (HOMA-IR) in-11377CC group were significantly lower than those in-11377CG +GG group(P<0.05 or P<O.01),but serum adiponectin level was higher in-11377CC group than that in -11377CG+GG group [(5.70 ±3.58 vs 4.72±2.34)mr/L,P<O.01].Variation at adiponectin gene-11377C→G was associated with increased CIMT in type 2 diabetic patients independent of serum adiponectin level(P= 0.037).Conclusion In type 2 diabetic patients of Fujian,the-11377C→G polymorphism of the adiponectin gene is associated with CIMT.
