中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2008年
11期
1444-1446
,共3页
大肠癌%C-myc%免疫组织化学%逆转录聚合酶链反应
大腸癌%C-myc%免疫組織化學%逆轉錄聚閤酶鏈反應
대장암%C-myc%면역조직화학%역전록취합매련반응
Colorectal carcinoma%C-myc%Immunohistochemistry%RT-PCR
目的 检测大肠癌组织中核不均一性核糖核蛋白(hnRNPA1)和原癌基因(C-myc)的表达,探讨两者在转录调控通路中的相互作用.方法 收集60例大肠癌组织标本,免疫组织化学及半定量聚合酶链反应法(RT-PCR)检测hnRNPA1和C-myc蛋白及基因表达情况.结果 60例大肠癌标本中有35例(58.3%)hnRNPA1蛋白阳性表达,且表达强度随大肠癌分化程度降低而递增(P<0.05);C-myc蛋白共有38例(63.3%)阳性表达;RT-PCR见hnRNPA1阳性标本占96.7%(58/60),C-myc基因阳性标本占91.7%(55/60),两者表达均随大肠癌的分化程度递减而增加(P<0.05);hnRNPA1和C-myc在基因和蛋白质水平呈正相关,r值为0.32和0.42.结论 hnRN-PA1和C-myc在不同分化程度的大肠癌中表达差异有统计学意义,两者在基因和蛋白表达水平上均呈正相关.
目的 檢測大腸癌組織中覈不均一性覈糖覈蛋白(hnRNPA1)和原癌基因(C-myc)的錶達,探討兩者在轉錄調控通路中的相互作用.方法 收集60例大腸癌組織標本,免疫組織化學及半定量聚閤酶鏈反應法(RT-PCR)檢測hnRNPA1和C-myc蛋白及基因錶達情況.結果 60例大腸癌標本中有35例(58.3%)hnRNPA1蛋白暘性錶達,且錶達彊度隨大腸癌分化程度降低而遞增(P<0.05);C-myc蛋白共有38例(63.3%)暘性錶達;RT-PCR見hnRNPA1暘性標本佔96.7%(58/60),C-myc基因暘性標本佔91.7%(55/60),兩者錶達均隨大腸癌的分化程度遞減而增加(P<0.05);hnRNPA1和C-myc在基因和蛋白質水平呈正相關,r值為0.32和0.42.結論 hnRN-PA1和C-myc在不同分化程度的大腸癌中錶達差異有統計學意義,兩者在基因和蛋白錶達水平上均呈正相關.
목적 검측대장암조직중핵불균일성핵당핵단백(hnRNPA1)화원암기인(C-myc)적표체,탐토량자재전록조공통로중적상호작용.방법 수집60례대장암조직표본,면역조직화학급반정량취합매련반응법(RT-PCR)검측hnRNPA1화C-myc단백급기인표체정황.결과 60례대장암표본중유35례(58.3%)hnRNPA1단백양성표체,차표체강도수대장암분화정도강저이체증(P<0.05);C-myc단백공유38례(63.3%)양성표체;RT-PCR견hnRNPA1양성표본점96.7%(58/60),C-myc기인양성표본점91.7%(55/60),량자표체균수대장암적분화정도체감이증가(P<0.05);hnRNPA1화C-myc재기인화단백질수평정정상관,r치위0.32화0.42.결론 hnRN-PA1화C-myc재불동분화정도적대장암중표체차이유통계학의의,량자재기인화단백표체수평상균정정상관.
Objective To detect heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) and C-myc mRNA and protein expression in colorectal cancer tissues for further study on mutual action of them in transcription regulation pathway. Methods In 60 coloroctal cancer tissue samples, the expression of hnRNPA1 and C-myc protein and mRNA was detected by using S-P immunohistochemistry and bemi-quan-tiffed reverse transcription polymerase chain reaction (RT-PCR). Results In 60 colorectal cancer tissue samples,35 cases (58.3%) were positive for the expression of hnRNPA1 protein and the expression in-tensity was increased with the decreases in differentiation degrees (P < 0.05) ; 38 cases were positive for the expression of C-mye protein. RT-PCR findings suggested that the positive expression rate of hnRNPA1 mRNA was 96.7% (58/60) and that of C-myc mRNA was 91.7% (55/60) ,and the expression of beth hnRNPAI and C-myc was increased with the decreases in differentiation degrees (P <0.05). There were positive correlations between hnRNPA1 and C-myc mRNA and protein (r = 0.32 and 0.42, respectively).Conclusion There was significant difference in the expression of hnRNPA1 and C-mye in colorectal cancers with different differentiation degrees, hnRNPA1 and C-myc were positively related in gene and pro-tein expression.
