CAJ | 학술논문

目的:探讨无淋巴结转移结肠癌微小核糖核酸(microRNA)表达谱,筛选与结肠癌早期发生、发展相关的特异性microRNA.方法:选取手术、病理证实未发生淋巴结转移的结肠癌及大于癌旁5 cm结肠组织标本各3例,抽提分离miRNA,与Agilent microRNA基因芯片杂交,并进行图像和数据分析.运用microRNA特异引物对差异表达的microRNA进行荧光定量实时聚合酶链反应(RT-RCR)验证.结果:筛选出在结肠癌中差异表达的microRNA有14个,其中12个microRNA表达水平增高,分别为miR-106b、miR-135b、miR-18a、miR-18b、miR-196b、miR-19a,miR-224、miR-335、miR-424、miR-20a*、miR-301b和miR-374a;2个microRNA表达水平降低,分别为miR-378和miR-378*.miR-106b和miR-19a下游存住符合要求的靶基凶数目众多.检测了芯片中显示表达上凋的miR-18a和miR-135b在3例结肠癌组织和癌旁组织中的表达,结果显示miR-18a和miR-135b在3例痛组织和癌旁组织中表达均上调;荧光定量RT-PCR结果与microRNA芯片的结果符合.结论:miR-106b、miR-135b、miR-18a、miR-18b、miR-196b等microRNA在结肠癌与癌旁组织中存在差异表达,预测其下游靶基因中大部分与肿瘤相关,提示它们可能在结肠癌早期发生、发展讨程审起着重要的调控作用.
목적:탐토무림파결전이결장암미소핵당핵산(microRNA)표체보,사선여결장암조기발생、발전상관적특이성microRNA.방법:선취수술、병리증실미발생림파결전이적결장암급대우암방5 cm결장조직표본각3례,추제분리miRNA,여Agilent microRNA기인심편잡교,병진행도상화수거분석.운용microRNA특이인물대차이표체적microRNA진행형광정량실시취합매련반응(RT-RCR)험증.결과:사선출재결장암중차이표체적microRNA유14개,기중12개microRNA표체수평증고,분별위miR-106b、miR-135b、miR-18a、miR-18b、miR-196b、miR-19a,miR-224、miR-335、miR-424、miR-20a*、miR-301b화miR-374a;2개microRNA표체수평강저,분별위miR-378화miR-378*.miR-106b화miR-19a하유존주부합요구적파기흉수목음다.검측료심편중현시표체상조적miR-18a화miR-135b재3례결장암조직화암방조직중적표체,결과현시miR-18a화miR-135b재3례통조직화암방조직중표체균상조;형광정량RT-PCR결과여microRNA심편적결과부합.결론:miR-106b、miR-135b、miR-18a、miR-18b、miR-196b등microRNA재결장암여암방조직중존재차이표체,예측기하유파기인중대부분여종류상관,제시타문가능재결장암조기발생、발전토정심기착중요적조공작용.
Objective To investigate microRNA expression profiles of colonic cancer without lymph node metastasis and identify the specific microRNAs associated with carcinogenesis of colon.Methods Cancerous and para-cancerous tissues (5 cm from cancer tissues of the colon) confirmed without lymph node metastasis were collected from 3 patients. The microRNAs were extracted and isolated by mirVana RNA kit. Hybridizations were made by applying the microRNAs to Agilent microRNA microarray. Data analysis was done by software of Feacture Extraction. The discovered microRNAs were confirmed by real time PCR assay. Results Twelve out of 14 microRNAs associated with colonic cancer were up-regulated in colonic tissues. They were miR-106b, miR-135b,miR-18a,miR-18b,miR-196b, miR-19a, miR-224, miR-335, miR-424, miR-20a' , miR-301b and miR-374a. The rest two of miR-378 and miR 378* were down-regulated. Two up-regulated microRNAs (miR-18a and miR-135b) were detected in 3 pairs of cancerous and para-cancerous tissues. The expression level of miR-18a and miR-135b were accordant with the results of real time PCR.Conclusions microRNA.such as miR-106b,miR-135b.miR-18a.miR-18b,miR-196b,ect,were differentially expressed between cancerous and para-cancerous tissues.Many of these target genes are supposed to participate in the process of multiple tunmorgenesis.These microRNAs play an important role in the carcinogenesis of colon.