中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2009年
2期
14-18
,共5页
食管鳞癌%热休克蛋白90α%基质金属蛋白酶-2%免疫组织化学
食管鱗癌%熱休剋蛋白90α%基質金屬蛋白酶-2%免疫組織化學
식관린암%열휴극단백90α%기질금속단백매-2%면역조직화학
Esophageal squamous cell carcinoma%Heat shock protein 90α%Matrix metalloprot-einase-2%Immunohistochemistry
目的 研究热休克蛋白90α(HSP 90α)和基质金属蛋白酶-2(MMP-2)在食管鳞状细胞癌(简称鳞癌)中的蛋白表达,分析二者表达的相关性及其与临床病理特征之间的关系.方法 采用免疫组织化学Elivision非生物素检测方法,对58例食管鳞癌、30例癌旁正常食管上皮组织切片进行染色,观察、评定HSP 90α和Survivin蛋白表达的阳性率及表达强度.结果 HSP 90α阳性产物主要位于细胞核,在食管鳞癌及食管正常上皮的阳性表达率分别为68.97%和13.33%.MMP-2阳性产物主要位于细胞核,在食管鳞癌及食管断端正常上皮的阳性表达率分别为70.69% 和16.67%.Survivin阳性产物主要位于细胞核,在食管鳞癌及食管断端正常上皮的阳性表达率分别为56.90%和6.67%.在食管鳞癌中,HSP90α表达与MMP-2表达呈正相关(rs=0.534,P=0.000).结论 HSP90α与MMP-2在食管鳞癌的发生、发展中起重要作用.
目的 研究熱休剋蛋白90α(HSP 90α)和基質金屬蛋白酶-2(MMP-2)在食管鱗狀細胞癌(簡稱鱗癌)中的蛋白錶達,分析二者錶達的相關性及其與臨床病理特徵之間的關繫.方法 採用免疫組織化學Elivision非生物素檢測方法,對58例食管鱗癌、30例癌徬正常食管上皮組織切片進行染色,觀察、評定HSP 90α和Survivin蛋白錶達的暘性率及錶達彊度.結果 HSP 90α暘性產物主要位于細胞覈,在食管鱗癌及食管正常上皮的暘性錶達率分彆為68.97%和13.33%.MMP-2暘性產物主要位于細胞覈,在食管鱗癌及食管斷耑正常上皮的暘性錶達率分彆為70.69% 和16.67%.Survivin暘性產物主要位于細胞覈,在食管鱗癌及食管斷耑正常上皮的暘性錶達率分彆為56.90%和6.67%.在食管鱗癌中,HSP90α錶達與MMP-2錶達呈正相關(rs=0.534,P=0.000).結論 HSP90α與MMP-2在食管鱗癌的髮生、髮展中起重要作用.
목적 연구열휴극단백90α(HSP 90α)화기질금속단백매-2(MMP-2)재식관린상세포암(간칭린암)중적단백표체,분석이자표체적상관성급기여림상병리특정지간적관계.방법 채용면역조직화학Elivision비생물소검측방법,대58례식관린암、30례암방정상식관상피조직절편진행염색,관찰、평정HSP 90α화Survivin단백표체적양성솔급표체강도.결과 HSP 90α양성산물주요위우세포핵,재식관린암급식관정상상피적양성표체솔분별위68.97%화13.33%.MMP-2양성산물주요위우세포핵,재식관린암급식관단단정상상피적양성표체솔분별위70.69% 화16.67%.Survivin양성산물주요위우세포핵,재식관린암급식관단단정상상피적양성표체솔분별위56.90%화6.67%.재식관린암중,HSP90α표체여MMP-2표체정정상관(rs=0.534,P=0.000).결론 HSP90α여MMP-2재식관린암적발생、발전중기중요작용.
Objective To study the expression of HSP 90α and MMP-2 proteins in esophageal squamous cell carcinoma(ESCC),and to analyze the relationships among expression,between protein expression and clinicopathologic factors.Methods Fifty-eight cases of ESCCs,30 cases of pericarcinoma normal tissues were immunohistochemically stained using HSP 90α,MMP-2 and Survivin monoclonal antibodies with ElivisionTM plus non-biotin detection system,the positive expression rate and expression intensity were evaluated.Results The HSP 90α protein positive expression rates were 68.97% and 13.33% in ESCC and normal epithelium respectively that mainly being expressed in nucleus,and the MMP-2 protein positive expression rates were 70.69% and 16.67% in ESCC and normal epithelium respectively that mainly being expressed in nucleus.HSP 90α protein expression was positively correlated with MMP-2.Conclusions HSP 90α and MMP-2 may play important roles in the carcinogenesis and development of ESCC.
