中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2012年
13期
130-132
,共3页
刘芳%朱家安%黄云霞%李殿城%郭微%芮蒙杰%徐宇虹%胡兵
劉芳%硃傢安%黃雲霞%李殿城%郭微%芮矇傑%徐宇虹%鬍兵
류방%주가안%황운하%리전성%곽미%예몽걸%서우홍%호병
造影剂%颈动脉%六氟化硫%全氟丙烷
造影劑%頸動脈%六氟化硫%全氟丙烷
조영제%경동맥%륙불화류%전불병완
Contrast media%Carotid arteries%Sulphur hexafluoride%Perfluoropropare
目的 对比研究六氟化硫微泡和全氟丙烷微泡的理化特性、携基因能力及在大鼠颈总动脉造影中的显影效果,旨在提供一种可动态实时监测的基因载体.方法 全氟丙烷和六氟化硫微泡均采用薄膜分散法制备,观察其形态、浓度、平均粒径及携B类清道夫受体I (SR-B I)能力,并对比研究其在大鼠颈总动脉显影效果及持续时间.结果 全氟丙烷阳离子脂质体微泡的粒径范围为0.712 ～5.56 μm,平均粒径1.312 μm,浓度为(3.12±0.39)×109/ml;六氟化硫阳离子脂质体微泡的粒径范围为0.915 ～96.86μm,平均粒径7.422μm,浓度为(7.43 ±0.28) ×107/ml.当SR-B I与这两种阳离子脂质体微泡的摩尔比为1:3时,微泡内SR-B I含量达到饱和.体内造影显示全氟丙烷微泡可快速充填颈总动脉管腔,持续时间达180 s以上.而六氟化硫微泡显影效果不明显.结论 与六氟化硫阳离子脂质体微泡相比,全氟丙烷阳离子脂质体微泡在体内造影有很好的显影效果,并且与SR-B I结合稳定,可望成为一种新型的载基因超声微泡造影剂.
目的 對比研究六氟化硫微泡和全氟丙烷微泡的理化特性、攜基因能力及在大鼠頸總動脈造影中的顯影效果,旨在提供一種可動態實時鑑測的基因載體.方法 全氟丙烷和六氟化硫微泡均採用薄膜分散法製備,觀察其形態、濃度、平均粒徑及攜B類清道伕受體I (SR-B I)能力,併對比研究其在大鼠頸總動脈顯影效果及持續時間.結果 全氟丙烷暘離子脂質體微泡的粒徑範圍為0.712 ～5.56 μm,平均粒徑1.312 μm,濃度為(3.12±0.39)×109/ml;六氟化硫暘離子脂質體微泡的粒徑範圍為0.915 ～96.86μm,平均粒徑7.422μm,濃度為(7.43 ±0.28) ×107/ml.噹SR-B I與這兩種暘離子脂質體微泡的摩爾比為1:3時,微泡內SR-B I含量達到飽和.體內造影顯示全氟丙烷微泡可快速充填頸總動脈管腔,持續時間達180 s以上.而六氟化硫微泡顯影效果不明顯.結論 與六氟化硫暘離子脂質體微泡相比,全氟丙烷暘離子脂質體微泡在體內造影有很好的顯影效果,併且與SR-B I結閤穩定,可望成為一種新型的載基因超聲微泡造影劑.
목적 대비연구륙불화류미포화전불병완미포적이화특성、휴기인능력급재대서경총동맥조영중적현영효과,지재제공일충가동태실시감측적기인재체.방법 전불병완화륙불화류미포균채용박막분산법제비,관찰기형태、농도、평균립경급휴B류청도부수체I (SR-B I)능력,병대비연구기재대서경총동맥현영효과급지속시간.결과 전불병완양리자지질체미포적립경범위위0.712 ～5.56 μm,평균립경1.312 μm,농도위(3.12±0.39)×109/ml;륙불화류양리자지질체미포적립경범위위0.915 ～96.86μm,평균립경7.422μm,농도위(7.43 ±0.28) ×107/ml.당SR-B I여저량충양리자지질체미포적마이비위1:3시,미포내SR-B I함량체도포화.체내조영현시전불병완미포가쾌속충전경총동맥관강,지속시간체180 s이상.이륙불화류미포현영효과불명현.결론 여륙불화류양리자지질체미포상비,전불병완양리자지질체미포재체내조영유흔호적현영효과,병차여SR-B I결합은정,가망성위일충신형적재기인초성미포조영제.
Objective To compare the characteristics and the SR-B I loading ability in vitro and to assess the enhancement effects on rat normal carotid artery of two kinds of lipid-coated cationic microbubbles (filled with perfluoropropare versus and sulphur hexafluoride).Methods Microbubbles filled with perfluoropropare and sulphur hexafluoride were prepared by film dispersion method.Its appearance,concentration,mean diameter,potential and the gene loading ability of the two kinds of microbubbles were detected.The enhancement effects and duration of the two kinds of microbubbles on rat normal carotid artery were compared.Results The mean diameter range of lipid-coated cationic microbubbles filled with perfluoropropare was 0.712-5.56 μm,the mean diameter was 1.312 μm and the concentration was(3.12 ±0.39) × 109/ml.The mean diameter range of lipid-coated cationic microbubbles filled with sulphur hexafluoride was 0.915-96.86 μm,the mean diameter was 7.422 μm and the concentration was( 7.43 ±0.28) × 107/ml.The molar ratio between gene and lipid-coated cationic microbubble was 1:3.Contrast imaging showed that microbubbles filled with perlluoropropare could fill the normal carotid artery quickly,and the duration was 180 s.However,microbubbles filled with sulphur hexafluoride did not have the apparent effect of enhanced ultrasonography.Conclusions Compared with lipid-coated cationic microbubbles filled with sulphur hexafluoride,microbubbles filled with perfluoropropare have a satisfactory effect of enhanced ultrasonography.And it can load gene stably,which may become a new ultrasound contrast agent as a vehicle for encapsulating with genes.
