中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2009年
7期
684-686
,共3页
游离胎儿DNA%DOWN综合征%实时定量PCR
遊離胎兒DNA%DOWN綜閤徵%實時定量PCR
유리태인DNA%DOWN종합정%실시정량PCR
Cell-free fetal DNA%DOWN syndrome%Real-time quantitative PCR
目的 探讨实时定量PCR方法检测妊娠DOWN综合征胎儿妇女血浆游离胎儿DNA的可行性.方法 SRY基因为胎儿游离DNA的标志,应用实时定量PCR分别检测10例妊娠DOWN综合征胎儿(男胎7例,女胎3例)妇女与20例妊娠正常胎儿(男胎14例,女胎6例)妇女血浆标本中游离胎儿DNA含量.结果 7例妊娠DOWN综合征男胎妇女SRY基因当量为(318.03±96.74)拷贝/ml(95%可信区间228.26～407.50拷贝/ml),妊娠正常男胎组SRY基因当量为(154.40±39.43)拷贝/ml(95%可信区间131.63～177.16拷贝/ml);2组差异有统计学意义(t=3.33,P=0.004).妊娠女胎组妇女血浆中SRY基因当量均为0拷贝/ml.结论 妊娠DOWN综合征男胎儿妇女血浆游离胎儿DNA的量较妊娠正常男胎儿妇女高.
目的 探討實時定量PCR方法檢測妊娠DOWN綜閤徵胎兒婦女血漿遊離胎兒DNA的可行性.方法 SRY基因為胎兒遊離DNA的標誌,應用實時定量PCR分彆檢測10例妊娠DOWN綜閤徵胎兒(男胎7例,女胎3例)婦女與20例妊娠正常胎兒(男胎14例,女胎6例)婦女血漿標本中遊離胎兒DNA含量.結果 7例妊娠DOWN綜閤徵男胎婦女SRY基因噹量為(318.03±96.74)拷貝/ml(95%可信區間228.26～407.50拷貝/ml),妊娠正常男胎組SRY基因噹量為(154.40±39.43)拷貝/ml(95%可信區間131.63～177.16拷貝/ml);2組差異有統計學意義(t=3.33,P=0.004).妊娠女胎組婦女血漿中SRY基因噹量均為0拷貝/ml.結論 妊娠DOWN綜閤徵男胎兒婦女血漿遊離胎兒DNA的量較妊娠正常男胎兒婦女高.
목적 탐토실시정량PCR방법검측임신DOWN종합정태인부녀혈장유리태인DNA적가행성.방법 SRY기인위태인유리DNA적표지,응용실시정량PCR분별검측10례임신DOWN종합정태인(남태7례,녀태3례)부녀여20례임신정상태인(남태14례,녀태6례)부녀혈장표본중유리태인DNA함량.결과 7례임신DOWN종합정남태부녀SRY기인당량위(318.03±96.74)고패/ml(95%가신구간228.26～407.50고패/ml),임신정상남태조SRY기인당량위(154.40±39.43)고패/ml(95%가신구간131.63～177.16고패/ml);2조차이유통계학의의(t=3.33,P=0.004).임신녀태조부녀혈장중SRY기인당량균위0고패/ml.결론 임신DOWN종합정남태인부녀혈장유리태인DNA적량교임신정상남태인부녀고.
Objective To investigate the application of real-time quantitative PCR in quantification of cell-free fetal DNA maternal plasma in patients bearing fetuses affected with DOWN syndrome. Methods Cell-free fetal DNA in maternal serum was isolated from 30 samples(7 male DOWN syndrome fetal ,3 female DOWN syndrome fe-tal,14 male euploid fetal,6 female euploid fetal). Cell-free fetal DNA levels in maternal serum were measured using real-time quantitative PCR using SRY as marker. Results The median cell-free fetal DNA levels in pregnant carry-ing male fetuses(n=7) and the controls (pregnant carrying male euploid fetuses,n=14)were 318.03±96.74 ge-nome-equivalents/ml and 154.40±39.43 genome-equivalents/ml of maternal serum,respectively (t=3.33,P=0.004 ),which was o in women with female fetuses. Conclusion The cell-free fetal DNA levels in pregnant women with DOWN syndrome fetuses are higher than that in pregnant women with normal fetuses.
