海洋科学
海洋科學
해양과학
MARINE SCIENCES
2009年
7期
81-86
,共6页
赵婷婷%李晓明%李景梅%李可%崔传明%李春顺%王斌贵
趙婷婷%李曉明%李景梅%李可%崔傳明%李春順%王斌貴
조정정%리효명%리경매%리가%최전명%리춘순%왕빈귀
多管藻(Polysiphonia urceolata)%内生真菌%化学成分%结构鉴定
多管藻(Polysiphonia urceolata)%內生真菌%化學成分%結構鑒定
다관조(Polysiphonia urceolata)%내생진균%화학성분%결구감정
Polysiphonia urceolata%Endophytic fungus%EN-22%chemical constituents%structure elucidation
对采自广西涠州岛近海多管藻(Poiysiphonia urceolata)分离到的一株内生真菌EN-22的次生代谢产物的化学成分进行了研究.利用正相硅胶柱层析、葡聚糖凝胶Sephadex LH-20柱层析、制备薄层层析(PTLC)以及重结晶等分离手段从该菌发酵液中分离得到15个化合物,通过一维、二维核磁共振技术、质谱技术等鉴定了所有化合物的结构,分别为:4-(4-喹啉)-4-羟基-2-丁酮(1),3-羟基-3-(2-氧丙基)吲哚-2-酮(2),3-吲哚乙醇(3),3-吲哚甲酸(4),2-羟基-3-吲哚丙酸(5),2-酮-3-吲哚乙醇(6),3,3-二吲哚-2-羟基-丙醇(7),β-咔啉(8),尿嘧啶(9),环-(S-脯氨酸-S-异亮氨酸)(10),(22E,24R)-3β,5α,9α-三羟基麦角甾-7,22-二烯-6-酮(11),(22E,24R)-5α,6α-环氧麦角甾-8,22-二烯-3β,7α-二醇(12),5α,6α-环氧-24(R)-甲基胆甾-7,22-二烯-3β-醇(13),胡萝卜苷(14),过氧化麦角甾醇(15);其中化合物1和2为新天然产物,并首次报道化合物1的碳谱数据.
對採自廣西潿州島近海多管藻(Poiysiphonia urceolata)分離到的一株內生真菌EN-22的次生代謝產物的化學成分進行瞭研究.利用正相硅膠柱層析、葡聚糖凝膠Sephadex LH-20柱層析、製備薄層層析(PTLC)以及重結晶等分離手段從該菌髮酵液中分離得到15箇化閤物,通過一維、二維覈磁共振技術、質譜技術等鑒定瞭所有化閤物的結構,分彆為:4-(4-喹啉)-4-羥基-2-丁酮(1),3-羥基-3-(2-氧丙基)吲哚-2-酮(2),3-吲哚乙醇(3),3-吲哚甲痠(4),2-羥基-3-吲哚丙痠(5),2-酮-3-吲哚乙醇(6),3,3-二吲哚-2-羥基-丙醇(7),β-咔啉(8),尿嘧啶(9),環-(S-脯氨痠-S-異亮氨痠)(10),(22E,24R)-3β,5α,9α-三羥基麥角甾-7,22-二烯-6-酮(11),(22E,24R)-5α,6α-環氧麥角甾-8,22-二烯-3β,7α-二醇(12),5α,6α-環氧-24(R)-甲基膽甾-7,22-二烯-3β-醇(13),鬍蘿蔔苷(14),過氧化麥角甾醇(15);其中化閤物1和2為新天然產物,併首次報道化閤物1的碳譜數據.
대채자엄서위주도근해다관조(Poiysiphonia urceolata)분리도적일주내생진균EN-22적차생대사산물적화학성분진행료연구.이용정상규효주층석、포취당응효Sephadex LH-20주층석、제비박층층석(PTLC)이급중결정등분리수단종해균발효액중분리득도15개화합물,통과일유、이유핵자공진기술、질보기술등감정료소유화합물적결구,분별위:4-(4-규람)-4-간기-2-정동(1),3-간기-3-(2-양병기)신타-2-동(2),3-신타을순(3),3-신타갑산(4),2-간기-3-신타병산(5),2-동-3-신타을순(6),3,3-이신타-2-간기-병순(7),β-잡람(8),뇨밀정(9),배-(S-포안산-S-이량안산)(10),(22E,24R)-3β,5α,9α-삼간기맥각치-7,22-이희-6-동(11),(22E,24R)-5α,6α-배양맥각치-8,22-이희-3β,7α-이순(12),5α,6α-배양-24(R)-갑기담치-7,22-이희-3β-순(13),호라복감(14),과양화맥각치순(15);기중화합물1화2위신천연산물,병수차보도화합물1적탄보수거.
From the fermentation culture of an endophytic fungus EN-22 that was derived from the marine red alga Polysiphonia urceolata, fifteen compounds were isolated by column chromatography separation on the normal phase silica gel and Sephadex LH-20, and by the preparative thin layer chromatography (PTLC) as well as by re-crystallization. Structures of all purified compounds were elucidated by analysis of their spectroscopic data including MS, 1D and 2D NMR to be 4-(4-Quinolyl)-4 hydroxy-2-butanone (1), 3-hydroxy-3-(2-oxopropyl)indo-lin-2-on (2), 2-(1H-indol-3-yl)ethanol (3), 1H-indole-3-carboxylic acid (4), 2-hydroxy-3-(1H-indol-3-yl)propanoic acid (5), 2-hydroxy-1-(1H-indol-3-yl)ethanone (6), 3,3-di(1H-indol-3-yl)propane-1,2-diol (7),β-carbolines (8), uracil (9), cyclo-(S-Pro-S-lle) (10), (22E,24R)-3β,5α,9α-trihydroxy-ergosta-7,22-diene-6-one (11), 22E,24R-Sα,6α-epoxyergosta-8,22-dien-3β,7a-diol (12), 5α,6α-epoxy-24(R)-methylcholesta-7,22-dien-3β-ol (13), daucosterol(14), and ergosterol peroxide (15). Compounds 1 and 2 were reported as new natural products.