复旦学报(医学版)
複旦學報(醫學版)
복단학보(의학판)
FUDAN UNIVERSITY JOURNAL OF MEDICAL SCIENCES
2009年
4期
490-494
,共5页
匡岩巍%王广强%石红梅%卢兖伟
劻巖巍%王廣彊%石紅梅%盧兗偉
광암외%왕엄강%석홍매%로연위
怀地黄%梓醇%高效液相%积累分布
懷地黃%梓醇%高效液相%積纍分佈
부지황%재순%고효액상%적루분포
Rehmannia glutinosa Liboseh. var. huechingensis Chaoet Schih%catalpol%HPLC%distribution and accumulation
目的 探索梓醇在怀地黄叶生长过程中的积累分布规律,为开发梓醇资源提供新思路.方法 采用高效液相色谱法测定新鲜药材中的梓醇含量.流动相:乙腈-水(0.6:99.4),检测波长:210nm,回归方程Y=377.26X-1.054 3.测定不同发育阶段的怀地黄叶的水分含量,换算成干燥品中梓醇的含量.结果 怀地黄叶与块根中均含有梓醇.地黄叶中的梓醇含量先迅速上升.在8、9月份的生长旺盛期达到最大值.然后逐渐降低直到地黄叶枯萎、凋谢.地黄块根中的梓醇随块根的增大而不断增多,一直持续到采收期收获.结论 怀地黄中的梓醇积累变化是植物生理调节的结果.怀地黄叶中含有大量梓醇,应根据其积累规律进行深入研究.以利于地黄的综合利用.
目的 探索梓醇在懷地黃葉生長過程中的積纍分佈規律,為開髮梓醇資源提供新思路.方法 採用高效液相色譜法測定新鮮藥材中的梓醇含量.流動相:乙腈-水(0.6:99.4),檢測波長:210nm,迴歸方程Y=377.26X-1.054 3.測定不同髮育階段的懷地黃葉的水分含量,換算成榦燥品中梓醇的含量.結果 懷地黃葉與塊根中均含有梓醇.地黃葉中的梓醇含量先迅速上升.在8、9月份的生長旺盛期達到最大值.然後逐漸降低直到地黃葉枯萎、凋謝.地黃塊根中的梓醇隨塊根的增大而不斷增多,一直持續到採收期收穫.結論 懷地黃中的梓醇積纍變化是植物生理調節的結果.懷地黃葉中含有大量梓醇,應根據其積纍規律進行深入研究.以利于地黃的綜閤利用.
목적 탐색재순재부지황협생장과정중적적루분포규률,위개발재순자원제공신사로.방법 채용고효액상색보법측정신선약재중적재순함량.류동상:을정-수(0.6:99.4),검측파장:210nm,회귀방정Y=377.26X-1.054 3.측정불동발육계단적부지황협적수분함량,환산성간조품중재순적함량.결과 부지황협여괴근중균함유재순.지황협중적재순함량선신속상승.재8、9월빈적생장왕성기체도최대치.연후축점강저직도지황협고위、조사.지황괴근중적재순수괴근적증대이불단증다,일직지속도채수기수획.결론 부지황중적재순적루변화시식물생리조절적결과.부지황협중함유대량재순,응근거기적루규률진행심입연구.이리우지황적종합이용.
Objective To explore the distribution and accumulation regularity of catalpol in leaves during the growth of Rehmannia glutinosa var. huechingensis. Methods HPLC method was used to determine the catalpol content in fresh medicinal materials with acetonitrile-water (0.6:99.4) as the mobile phase and 210 nm as detection wavelength. Regression equation was Y = 377.26X - 1. 054 3. The water contents of the leaves at different developmental stages were also determined, so that the catalpol content could be achieved on dry matter basis.Results Both leaves and root tubers contained catalpol. The content of catalpol in leaves rised up to peak rapidly in August or September and then degraded gradually until leaves wither and fall. The content of catalpol in root tubers increased with the growth of root tubers and reached peak until harvest. Conclusions The variation of catalpol is caused by the physiological regulation of plants. The leaves contain a considerable amount of catalpol. Further study is needed to benefit the comprehensive utilization of Rehmannia glutinosa Libosch.