CAJ | 학술논문

为了了解白细胞介素-1基因在中国重庆市汉族健康人群中的分布及其与不同种族比较的特点,采用了聚合酶链反应一限制性片段长度多态(PCR-RFLP)的方法,对140名重庆市汉族健康者的IL-1B-511基因多态性和IL1RN第2内含子可变数目串联重复序列多态性进行检测,并结合相关文献进行了不同种族间的分析比较.结果表明重庆市汉族健康人群中IL-1B-511的各基因型频率为C/T型0.58、T/T型0.50、C/C型0.32,与西班牙白种人相比,重庆地区汉族人IL-1B-511等位基因频率存在明显差异(P＜0.05).IL-IRN的各基因型频率为1/1型0.93、1/2型0.05、1/4型0.01、4/4型O.01,与西班牙白种人及南非黑种人相比,重庆地区汉族人IL-IRN等位基因频率存在明显差异(P＜0.05).由此可以得出重庆地区汉族人群IL-1B-511位点存在C/T多态性和IL-IBN基因的第2号内含子存在可变数目串联重复序列多态性,其在不同种族间的分布存在着差异.
위료료해백세포개소-1기인재중국중경시한족건강인군중적분포급기여불동충족비교적특점,채용료취합매련반응일한제성편단장도다태(PCR-RFLP)적방법,대140명중경시한족건강자적IL-1B-511기인다태성화IL1RN제2내함자가변수목천련중복서렬다태성진행검측,병결합상관문헌진행료불동충족간적분석비교.결과표명중경시한족건강인군중IL-1B-511적각기인형빈솔위C/T형0.58、T/T형0.50、C/C형0.32,여서반아백충인상비,중경지구한족인IL-1B-511등위기인빈솔존재명현차이(P＜0.05).IL-IRN적각기인형빈솔위1/1형0.93、1/2형0.05、1/4형0.01、4/4형O.01,여서반아백충인급남비흑충인상비,중경지구한족인IL-IRN등위기인빈솔존재명현차이(P＜0.05).유차가이득출중경지구한족인군IL-1B-511위점존재C/T다태성화IL-IBN기인적제2호내함자존재가변수목천련중복서렬다태성,기재불동충족간적분포존재착차이.
To investigate the gene polymorphism distribution of interleukin-1 in the Han population in Chongqing,and to compare and analyze the distributions of SNP among different races,PCR-restriction fragment length polymorphism(PCR-RELP)was used to detectthe IL-1B-511,IL-IRN polymorphism in 140 healthy people of Han nationality in Chongqing.It was helpful for making comparative analysis in different ethnic people with the literature.Results showed that among healthy people of Hall nationality in Chongqing,the frequencies of IL-1B-511 genotype of C/T,T/T and C/C were 0.58,0.50 and 0.32,respectively.Compared with the caucasian in Spain,the difference of IL-1B-511 gene allele frequency distribution was statistically significant(P＜0.05).The frequencies of IL1RN genotype of 1/1,1/2,1/4 and 4/4 were 0.93,0.05,0.01 and 0.01,respectively.Compared with the caucasian in Spain and melanoderm in South Africa,thedifference of IL-1RN gene allelefrequency distribution was statistically significant(P＜0.05).It indicated that in the Han population in Chongqing,the IL-1B-511 site exists C/T polymorphism and IL-1RN gene in the exon 2 exists variable number of tandem repeat polymorphism and their distribution among different ethnic people is different.