中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2010年
3期
312-316
,共5页
滋肾阴泻相火中药%环境内分泌干扰物%雌激素受体
滋腎陰瀉相火中藥%環境內分泌榦擾物%雌激素受體
자신음사상화중약%배경내분비간우물%자격소수체
Chinese herbs for nourishing Shen-yin and removing Xiang-fire%environmental endocrine disruptor%estrogen receptor
目的 观察滋肾阴泻相火中药对壬基酚及双酚A染毒大鼠子宫、卵巢雌激素受体(ER)基因和蛋白表达的影响,探讨该中药拮抗环境内分泌干扰物(EEDs)拟雌激素活性的作用机制.方法 3周龄雌性sD大鼠染毒组单纯喂饲壬基酚或壬基酚与双酚A联合喂饲,治疗组以滋肾阴泻相火中药与染毒物同时喂饲,对照组喂饲溶剂玉米油,给药15天,分别检测子宫湿重及脏器系数、子宫及卵巢ER基因和蛋白表达水平.结果 染毒组与对照组比较,子宫湿重及子宫系数增加,ER蛋白表达显著上调;卵巢Era、Erβ的基因表达及蛋白水平显著上调(P＜0.05).治疗组与染毒组比较,子宫湿重、子宫系数降低,ER的蛋白表达显著下调;卵巢Era、Erβ的基因表达及蛋白水平显著下调(P＜0.05).结论 EEDs对生殖器官有显著的拟雌激素作用,而滋肾阴泻相火中药对EEDs的拟雌激素活性具有显著的拮抗作用.中药的治疗干预可使生殖器官ER基因、蛋白表达显著下调,从而显著降低生殖器官对EEDs的敏感性,这可能是所用中药有效拮抗EEDs拟雌激素活性的主要作用机制之一.
目的 觀察滋腎陰瀉相火中藥對壬基酚及雙酚A染毒大鼠子宮、卵巢雌激素受體(ER)基因和蛋白錶達的影響,探討該中藥拮抗環境內分泌榦擾物(EEDs)擬雌激素活性的作用機製.方法 3週齡雌性sD大鼠染毒組單純餵飼壬基酚或壬基酚與雙酚A聯閤餵飼,治療組以滋腎陰瀉相火中藥與染毒物同時餵飼,對照組餵飼溶劑玉米油,給藥15天,分彆檢測子宮濕重及髒器繫數、子宮及卵巢ER基因和蛋白錶達水平.結果 染毒組與對照組比較,子宮濕重及子宮繫數增加,ER蛋白錶達顯著上調;卵巢Era、Erβ的基因錶達及蛋白水平顯著上調(P＜0.05).治療組與染毒組比較,子宮濕重、子宮繫數降低,ER的蛋白錶達顯著下調;卵巢Era、Erβ的基因錶達及蛋白水平顯著下調(P＜0.05).結論 EEDs對生殖器官有顯著的擬雌激素作用,而滋腎陰瀉相火中藥對EEDs的擬雌激素活性具有顯著的拮抗作用.中藥的治療榦預可使生殖器官ER基因、蛋白錶達顯著下調,從而顯著降低生殖器官對EEDs的敏感性,這可能是所用中藥有效拮抗EEDs擬雌激素活性的主要作用機製之一.
목적 관찰자신음사상화중약대임기분급쌍분A염독대서자궁、란소자격소수체(ER)기인화단백표체적영향,탐토해중약길항배경내분비간우물(EEDs)의자격소활성적작용궤제.방법 3주령자성sD대서염독조단순위사임기분혹임기분여쌍분A연합위사,치료조이자신음사상화중약여염독물동시위사,대조조위사용제옥미유,급약15천,분별검측자궁습중급장기계수、자궁급란소ER기인화단백표체수평.결과 염독조여대조조비교,자궁습중급자궁계수증가,ER단백표체현저상조;란소Era、Erβ적기인표체급단백수평현저상조(P＜0.05).치료조여염독조비교,자궁습중、자궁계수강저,ER적단백표체현저하조;란소Era、Erβ적기인표체급단백수평현저하조(P＜0.05).결론 EEDs대생식기관유현저적의자격소작용,이자신음사상화중약대EEDs적의자격소활성구유현저적길항작용.중약적치료간예가사생식기관ER기인、단백표체현저하조,종이현저강저생식기관대EEDs적민감성,저가능시소용중약유효길항EEDs의자격소활성적주요작용궤제지일.
Objective To investigate the effect of Chinese herbs for nourishing Shen-yin and removing Xiang-fire (NYRF) on estrogen receptor (ER) expression in uterus and ovary of rats contaminated with nonylphenol (NP) or its bisphenol A (BPA) mixture,for exploring the action mechanism of NYRF in antagonizing the estrogen-mimetic activity of environmental endocrine disruptors (EEDs).Methods EEDs contaminated female SD rats,3-week old,were divided into two groups,the treated group fed with NYRF and the control group with corn oil during the same period of contaminating for 15 days.The wet weight (WW) and organ coefficient (OC) of uterus in rats,as well as the ER protein and mRNA expressions in rat's uterus and ovary were detected and compared.Resuits AS compared with normal range,WW and OC increased significantly in the contaminated rats of the control group,with significantly down-regulated ER protein expression in uterus,and expressions of ERa and ERβ gene and protein in ovary(P＜0.05).While in the treated group,the above-mentioned abnormalities of various indictors were markedly reversed to a certain extent(P＜0.05).Conclusion EEDs show estrogenic-mimetic action on productive organs,which could be antagonized by NYRF,resulting in the down-regulated mRNA and protein expressions of ER in reproductive organs,so as to reduce the sensibility of reproductive organs to EEDs,which is probably one of the acting mechanisms of NYRF.
