CAJ | 학술논문

建立了以液相色谱串联四极杆质谱(HPLC-MS/MS)检测猪肝猪肉中齐帕特罗、马布特罗、班布特罗、马贲特罗的分析方法.样品经MCX柱净化浓缩后,用C18液相色谱柱分离,通过电喷雾(ESI)离子源电离,多反应监测(MRM)作定性和定量分析.在0.25～50μg/L的范围内线性良好(r>0.999).在0.5、4.0、10μg/kg添加水平下,回收率为70.5%～91.9%,相对标准偏差为3.7%～7.4%.方法检测限(S/N=5)可达0.5 μg/kg.
건립료이액상색보천련사겁간질보(HPLC-MS/MS)검측저간저육중제파특라、마포특라、반포특라、마분특라적분석방법.양품경MCX주정화농축후,용C18액상색보주분리,통과전분무(ESI)리자원전리,다반응감측(MRM)작정성화정량분석.재0.25～50μg/L적범위내선성량호(r>0.999).재0.5、4.0、10μg/kg첨가수평하,회수솔위70.5%～91.9%,상대표준편차위3.7%～7.4%.방법검측한(S/N=5)가체0.5 μg/kg.
The HPLC-MS/MS method was presented to analyze zilpateml,mabuteml,bambuterol and maponteml in liver of pig and pork.The sample was purified and concentrated by MCX column,chromatographic conditions included a C18 column.Qualitative and quantitative analysis which ionized by electrospray ionization interface(ESI)were carried on through MRM mode.There was a good linear correlation in the range of 0.25～50 μg/L (r>0.999).The recoveries ranged from 70.5%to 91.9% At the add concentration level of 0.5、4.0、10 μg/kg,the RSD were between 3.7%a nd 7.4%.Its limit of detection reached 0.5 μg/kg.