CAJ | 학술논문

目的探讨吡非尼酮(PFD)对大鼠矽肺纤维化的抑制作用.方法 75只SD雄性大鼠,随机分成未处理对照组、生理盐水组、生理盐水+PFD组、SiO2组、SiO2+PFD组,每组15只,采用非暴露式气管内染尘法,SiO2组和SiO2+PFD组注入SiO2粉尘悬液(25 mg/ml),生理盐水组和生理盐水+PFD组注入等量生理盐水,染尘后第2天灌胃给予PFD(50 mg/kg),分别观察7、21、42 d后处死.HE、VG和Foot染色观察肺组织的病理形态学改变并进行病理分级,测定肺组织中羟脯氨酸(HYP)的含量.结果大鼠肺组织病理观察显示,SiO2+PFD组肺组织纤维化程度比同期SiO2组明显减轻,胶原纤维形成缓慢,矽结节分级评分降低.第42天时,SiO2+PFD组大鼠肺组织HYP含量[(0.75±0.12)mg/g肺组织]比SiO2组[(1.19±0.17)mg/g肺组织]明显降低,差异有统计学意义(P＜0.05).结论 PFD能降低大鼠矽肺纤维化程度并减少肺组织中HYP的含量,对实验性大鼠矽肺纤维化具有抑制作用.
목적 탐토필비니동(PFD)대대서석폐섬유화적억제작용.방법 75지SD웅성대서,수궤분성미처리대조조、생리염수조、생리염수+PFD조、SiO2조、SiO2+PFD조,매조15지,채용비폭로식기관내염진법,SiO2조화SiO2+PFD조주입SiO2분진현액(25 mg/ml),생리염수조화생리염수+PFD조주입등량생리염수,염진후제2천관위급여PFD(50 mg/kg),분별관찰7、21、42 d후처사.HE、VG화Foot염색관찰폐조직적병리형태학개변병진행병리분급,측정폐조직중간포안산(HYP)적함량.결과 대서폐조직병리관찰현시,SiO2+PFD조폐조직섬유화정도비동기SiO2조명현감경,효원섬유형성완만,석결절분급평분강저.제42천시,SiO2+PFD조대서폐조직HYP함량[(0.75±0.12)mg/g폐조직]비SiO2조[(1.19±0.17)mg/g폐조직]명현강저,차이유통계학의의(P＜0.05).결론 PFD능강저대서석폐섬유화정도병감소폐조직중HYP적함량,대실험성대서석폐섬유화구유억제작용.
Objective To investigate whether pirfenidone(PFD) presents the antifibrotic effect in silicosis of rats. Methods SD rats were randomly divided into five groups: the non-treat group, the normal saline group, the normal saline + PFD group, the SiO2 group, the SiO2 + PFD group. Rats except in the non-treat group were intratracheally instilled with SiO2(25 mg/ml) or normal saline. The rats in normal saline + PFD group and the SiO2 + PFD group were given PFD (50 rmg/kg) orally the next day after instillation and throughout the study.Rats were respectively sacrifced 7, 21, 42 days after instillation. The pathology changes were evaluated by Haematoxylin-eosin (HE), Van Gieson and Foot staining, and the hydroxyproline (HYP) content of pulmonary tissue was determined. Results Compared with the SiO2 group, PFD could relieve the fibrotic changes in the lungs of rats. The fibrotic degree in silicotic lesions of lungs was lower in the SiO2 + PFD group than that of SiO2 group. The HYP content in the lungs of the SiO2 + PFD group [(0.75±0.12) mg/g] was signifcantly lower than that of the SiO2 group [( 1.19±0.17 ) mg/g] at 42 days after instillation(P＜0.05 ). Conclusion These data support that PFD has an antifibrotic effect against SiO2 induced lung fibrosis in rats, Which appears to be changing collagen accumulation and inhibiting pulmonary fibrosis.