CAJ | 학술논문

目的研究在单侧隐睾大鼠背根神经节中,生殖股神经神经核的改变.方法应用辣根过氧化酶(HRP)逆行示踪与免疫荧光相结合的双重技术,观察注射17-β雌二醇获得隐睾大鼠动物模型GFN传导及其递质免疫反应细胞的变化.取单侧隐睾大鼠12只设为A组;取12只单侧隐睾大鼠在出生后第30天切断隐睾侧生殖股神经(GFN)设为B组;另取正常同日龄大鼠10只设为C组.分别在日龄第58天隐睾侧GFN末梢用HRP逆行示踪.48 h后对SD大鼠灌注固定后,取大鼠的T12～L3脊髓及背根神经结,脱水后冰冻切片显色,并用免疫荧光标记CGRP.结果 HRP标记的生殖股神经在L1,L2水平的背根神经节感觉核中发现,A组HRP阳性神经元表达平均灰度和阳性单位较B组和C组要高有统计学意义(P<0.05),B组和C组比较(P＞0.05);CGRP免疫荧光结果中A组较B组、C组表达要少(P<0.05).结论单侧隐睾大鼠生殖股神经感觉核神经元增多,运动核相对减少,分泌的神经递质CGPR减少.
목적 연구재단측은고대서배근신경절중,생식고신경신경핵적개변.방법 응용랄근과양화매(HRP)역행시종여면역형광상결합적쌍중기술,관찰주사17-β자이순획득은고대서동물모형GFN전도급기체질면역반응세포적변화.취단측은고대서12지설위A조;취12지단측은고대서재출생후제30천절단은고측생식고신경(GFN)설위B조;령취정상동일령대서10지설위C조.분별재일령제58천은고측GFN말소용HRP역행시종.48 h후대SD대서관주고정후,취대서적T12～L3척수급배근신경결,탈수후빙동절편현색,병용면역형광표기CGRP.결과 HRP표기적생식고신경재L1,L2수평적배근신경절감각핵중발현,A조HRP양성신경원표체평균회도화양성단위교B조화C조요고유통계학의의(P<0.05),B조화C조비교(P＞0.05);CGRP면역형광결과중A조교B조、C조표체요소(P<0.05).결론 단측은고대서생식고신경감각핵신경원증다,운동핵상대감소,분비적신경체질CGPR감소.
Objective To study the changes of genitofemoral nerve (GFN) nuclear in dorsal root ganglion of unilateral cryptorchid rats. Methods Using retrograde tracing of horseradish peroxidase (HRP) and immunofluorescent double labeling, we observed GFN nucleus and calcitonin gene related peptide immunoreactivity (CGRP-IR) cells of the unilateral crytorchid rats induced by 17-β estradiol. Group A (n=12), unilateral cryptorchid; Group B (n=12), unilateral cryptorchid combined with GFN division at 30 d after birth, and Group C (n=10), control group. The GFN proximal ends of the 3 groups were labeled with HRP at post-birth 58 d. The rats were killed 48 h later, and the ganglia and spinal cord were removed, serial sections frozen in cryostat were cut, and HRP labeled neurons were counted and the CGRP-IR cells were double fluorescently labeled with antibody to CGRP and FICT.Results HRP-positive GFN was found in sensory nuclei of L1, L2 dorsal root ganglias and CGRP-IR cells in motor nuclei. The mean gray scale and positive unit of HRP-positive neurons of group A was obvious higher than that of group B and C (P＜0.05), but CGRP-IR cells were significantly less (P＜0.05). However, compared with group C, in group B HRP-positive neurons and CGRP-IR cells were not statistically significant different (P ＞0.05). Conclusions In rats with contralateral testicular impairment induced by unilateral cryptorchid, the sensory neurons increase and motor neurons decrease and its secretion of neurotransmitter CGRP is inhibited.