中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2008年
6期
495-497
,共3页
蔡玮%孙明波%陈洪波%洪超%宋晓艳%余磊%姜述德%廖国阳%杨净思
蔡瑋%孫明波%陳洪波%洪超%宋曉豔%餘磊%薑述德%廖國暘%楊淨思
채위%손명파%진홍파%홍초%송효염%여뢰%강술덕%료국양%양정사
脊髓灰质炎%疫苗,蛋白质类
脊髓灰質炎%疫苗,蛋白質類
척수회질염%역묘,단백질류
Poliovmyclitis%Vaccine,Proteins
目的 建立Sabin株脊髓灰质病毒灭活疫苗(Sabin strain inactivated poliovims vaccine,SabinIPV)中微量蛋白质含量测定的方法.方法 采用lowry法结合三氯乙酸沉淀法测定试样中的蛋白质含量,摸索并优化各个实验条件.结果 该方法能够排除Sabin IPV疫苗样品中游离氨基酸、多肽和酚红指示剂等的干扰,线性范围为2.5-40μg/ml,r=0.9998;加标平均同收率为95.32%,批内和批间变异系数均小于10%.结论 三氯乙酸沉淀法结合Lowry法的改良方法可以用于测定生物制品中微量蛋白含量.
目的 建立Sabin株脊髓灰質病毒滅活疫苗(Sabin strain inactivated poliovims vaccine,SabinIPV)中微量蛋白質含量測定的方法.方法 採用lowry法結閤三氯乙痠沉澱法測定試樣中的蛋白質含量,摸索併優化各箇實驗條件.結果 該方法能夠排除Sabin IPV疫苗樣品中遊離氨基痠、多肽和酚紅指示劑等的榦擾,線性範圍為2.5-40μg/ml,r=0.9998;加標平均同收率為95.32%,批內和批間變異繫數均小于10%.結論 三氯乙痠沉澱法結閤Lowry法的改良方法可以用于測定生物製品中微量蛋白含量.
목적 건립Sabin주척수회질병독멸활역묘(Sabin strain inactivated poliovims vaccine,SabinIPV)중미량단백질함량측정적방법.방법 채용lowry법결합삼록을산침정법측정시양중적단백질함량,모색병우화각개실험조건.결과 해방법능구배제Sabin IPV역묘양품중유리안기산、다태화분홍지시제등적간우,선성범위위2.5-40μg/ml,r=0.9998;가표평균동수솔위95.32%,비내화비간변이계수균소우10%.결론 삼록을산침정법결합Lowry법적개량방법가이용우측정생물제품중미량단백함량.
Objective To establish a method for the content determination of protein in Sabin IPV.Methods Using lowry method combined with being precipitated by trichloroacetic acid to determine the content of protein in Sabin IPV. Changing different conditions to optimize the experiment to establish a improved lowry method. And the sample recovery test was also conducted. Results The method can exclude the interference of free aminoacid,phenols and some other additives.The calibration curve was in good linearity of Protein within the range of 2.5 μg/ml-40 μg/ml, r = 0.9998. Under the best conditions,the mean recovery was 95.32% ,the CV in a batch and between batches were beth < 10%. Conclusion The method can be used to determine the micro content of protein in vaccines.
