吉林省2009—2010年引起手足口病流行的肠道病毒71型基因特性的研究
길림성2009—2010년인기수족구병류행적장도병독71형기인특성적연구
Multiple viral transmission chains of enterovirus 71 co-circulated in Jilin Province during 2009-2010
  • 万方数据
    中华实验和临床病毒学杂志 중화실험화림상병독학잡지
    CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
    2012年 4期 273-275 ,共3页

    周剑惠%王爽%魏雷雷%吴静%齐忠%刘红%苟伟民%陈创%檀晓娟%张勇%张燕%许文波

    주검혜%왕상%위뢰뢰%오정%제충%류홍%구위민%진창%단효연%장용%장연%허문파

    手足口病%肠道病毒属%疾病传播%流行病学 手足口病%腸道病毒屬%疾病傳播%流行病學
    수족구병%장도병독속%질병전파%류행병학
    Hand foot and mouth disease%Enterovirus%Disease transmission%Epidemiology
    目的 分析引起吉林省2009 -2010年手足口病(hand foot and mouth disease,HFMD)流行的肠道病毒71型的基因进化特征.方法 选择31株2009-2010年分离于吉林省8个地市(州)HFMD病例的EV71代表株,对VP1编码区基因进行逆转录-聚合酶链反应扩增、扩增产物的序列测定,使用Bioedit软件和MEGA 4.0软件对VP1基因进行同源性、基因亲缘关系分析.结果 吉林省2009-2010年的31株EV71分离株均属于C4a基因亚型,31株EV71株在VP1区的核苷酸的同源性在94.5％～100％之间,分属于5个分支,其中25株病毒(分离于8个地市)形成一个大的分支,属于绝对优势传播链,其余6株形成4个小分支.结论 2009-2010年在吉林省内有多个EV71C4a基因型的传播链同时存在,其中分支1为绝对优势传播链,在8个地市持续传播,引起HFMD的暴发流行.
    목적 분석인기길림성2009 -2010년수족구병(hand foot and mouth disease,HFMD)류행적장도병독71형적기인진화특정.방법 선택31주2009-2010년분리우길림성8개지시(주)HFMD병례적EV71대표주,대VP1편마구기인진행역전록-취합매련반응확증、확증산물적서렬측정,사용Bioedit연건화MEGA 4.0연건대VP1기인진행동원성、기인친연관계분석.결과 길림성2009-2010년적31주EV71분리주균속우C4a기인아형,31주EV71주재VP1구적핵감산적동원성재94.5％～100％지간,분속우5개분지,기중25주병독(분리우8개지시)형성일개대적분지,속우절대우세전파련,기여6주형성4개소분지.결론 2009-2010년재길림성내유다개EV71C4a기인형적전파련동시존재,기중분지1위절대우세전파련,재8개지시지속전파,인기HFMD적폭발류행.
    Objective To analyze the genetic characterization of enterovirus type71 ( EV71 )associated with hand foot and mouth disease (HFMD) epidemics in Jilin province,during 2009-2010.Methods Randomly selected 31 representative EV71 strains from the eases of 8 prefectures to amplify and sequences of VP1 genes of EV71 strains,and analyzed with Bioedit and Mega4.0 program.Results All representative 31 EV71 strains belong to C4a subgenotype,the homology of nucleotide in VP1 region among the 31 EV71 strains were 94.5％-100.0％,and were clustered into 5 transmission chains respectively.25 strains out of 31 strains were associated with a predominant transmission chain,and circulating in 8 prefectures,while other 6 strains clustered into 4 lineages.Conclusion Multiple transmission chains of EV71 C4a subgenotype were co-circulating in Jilin province during 2009-2010,and a predominant transmission chain was circulating in 8 prefectures,associated with HFMD outbreaks of Jilin province.
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