CAJ | 학술논문

目的探讨CXCL16/CXCR6在肺癌的表达模式及其对肺癌细胞系生物学行为的影响.方法免疫组织化学技术分析CXCL16/CXCR6蛋白在人肺癌组织的表达；免疫细胞化学分析CXCL16/CXCR6在3种肺癌细胞系A549、95D和H292的表达；MTT试验及迁移、侵袭试验分别分析CXCL16时A549、95D和H292细胞体外增殖活力和侵袭能力的调节作用.结果人肺癌组织高表达CXCR6和CXCL16蛋白；A549、95D和H292细胞均表达CXCL16和CXCR6蛋白；外源性CXCL16可明显促进A549、95D和H292细胞的体外增殖活力和侵袭能力,且CXCL16中和抗体可以有效阻断CXCL16蛋白对3种肺癌细胞系的刺激作用.结论 CXCL16/CXCR6可能是参与肺癌侵袭转移的分子.
목적 탐토CXCL16/CXCR6재폐암적표체모식급기대폐암세포계생물학행위적영향.방법 면역조직화학기술분석CXCL16/CXCR6단백재인폐암조직적표체；면역세포화학분석CXCL16/CXCR6재3충폐암세포계A549、95D화H292적표체；MTT시험급천이、침습시험분별분석CXCL16시A549、95D화H292세포체외증식활력화침습능력적조절작용.결과 인폐암조직고표체CXCR6화CXCL16단백；A549、95D화H292세포균표체CXCL16화CXCR6단백；외원성CXCL16가명현촉진A549、95D화H292세포적체외증식활력화침습능력,차CXCL16중화항체가이유효조단CXCL16단백대3충폐암세포계적자격작용.결론 CXCL16/CXCR6가능시삼여폐암침습전이적분자.
Objective To explore the role of CXCL16/CXCR6 axis on the metastasis of human lung cancer.Methods Immunohistochemistry and immunocytochemistry analysis were performed to detect the expression of CXCL16/CXCR6 in human lung cancer samples as well as A549,95D and H292 cell lines,respectively.The effects of CXCL16 on the viability and invasiveness of the three lung cancer cell lines were examined by MTT and in vitro invasion assay,respectively.Results Human native lung cancer cells co-expressed CXCR6 and CXCL16 protein.Compared to the normal lung tissues,there was a stronger specific staining for both CXC16 and CXCR6 protein in the lung cancer tissues.Three kinds of lung cancer cell lines,including A549,95D and H292,all expressed CXCL16 and CXCR6 protein.Furthermore,human recombinant CXCL16 significantly promoted the viability and invasiveness of A549,95D and H292 cells.The stimulated action of CXCL16 on lung cancer cell lines could be effectively blocked by CXCL16 neutralizing antibody.Conclusion CXCL16 and CXCR6 protein are co-expressed in human native lung cancer.CXCL16 is able to promote the viability and invasiveness of lung cancer cell lines,which might be the molecular mechanisms on the metastasis of lung cancer.