中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2011年
3期
192-196
,共5页
熊曾%刘进康%周漠玲%胡成平%周晖%陈伟
熊曾%劉進康%週漠玲%鬍成平%週暉%陳偉
웅증%류진강%주막령%호성평%주휘%진위
癌,非小细胞肺%EphB4%ephrinB2%多层螺旋CT%血液灌注
癌,非小細胞肺%EphB4%ephrinB2%多層螺鏇CT%血液灌註
암,비소세포폐%EphB4%ephrinB2%다층라선CT%혈액관주
Carcinoma,non-small cell lung%EphB4%ephrinB2%Multi-slice spiral CT%Blood flow perfusion
目的 探讨非小细胞肺癌(NSCLC)组织中EphB4和ephrinB2的表达及其与CT肺灌注成像的关系.方法 对31例NSCLC患者的肺内单发结节行CT灌注成像,获得血流量(BF)、血容量(BV)和最高增强值(PEI)数据.采用免疫组化SP法检测EphB4和ephrinB2在肿瘤细胞与间质血管中的表达.运用Spearman相关分析与趋势检验探讨EphB4和ephrinB2蛋白表达与NSCLC临床病理特征、灌注参数的关系.结果 31例NSCLC组织中,22例(71.0%)EphE4表达阳性,26例(83.9%)ephrinB2表达阳性,明显高于内对照(分别为24.0%和20.3%,P＜0.01).EphB4和ephrinB2的表达在肿瘤细胞中有一致性,在肿瘤间质血管中有差异性.EphB4和ephrinB2蛋白表达强度与淋巴结转移均呈低度正相关(r=0.370,P=0.045;r=0.362,P=0.047).31例NSCLC患者BF为(42.3±10.2)ml·100 mg-1·min-1,BV为(9.0±3.9)mL/100 g,PEI为(21.6±8.4)HU.相关分析显示,在NSCLC肿瘤细胞中,EphB4的表达强度分别与BF和BV呈负相关(r=-0.452,P=0.011;r=-0.408,P=0.023),与PEI无关.ephrinB2的表达强度分别与BF和BV呈正相关(r=0.516,P=0.003;r=0.448,P=0.013),与PEI无关.EphB4表达强阳性组和阳性组的BF与BV值均显著低于EphB4表达阴性组(均P＜0.01).ephrinB2表达强阳性组的BF值显著高于ephrinB2表达阳性组和阴性组(均P＜0.01),ephrinB2表达强刚性组的BV值显著高于ephrinB2表达阴性组(P＜0.01).结论 灌注成像反映功能性管腔化血管的密度差异,这种差异同时取决于管腔化血管的数量与质量.
目的 探討非小細胞肺癌(NSCLC)組織中EphB4和ephrinB2的錶達及其與CT肺灌註成像的關繫.方法 對31例NSCLC患者的肺內單髮結節行CT灌註成像,穫得血流量(BF)、血容量(BV)和最高增彊值(PEI)數據.採用免疫組化SP法檢測EphB4和ephrinB2在腫瘤細胞與間質血管中的錶達.運用Spearman相關分析與趨勢檢驗探討EphB4和ephrinB2蛋白錶達與NSCLC臨床病理特徵、灌註參數的關繫.結果 31例NSCLC組織中,22例(71.0%)EphE4錶達暘性,26例(83.9%)ephrinB2錶達暘性,明顯高于內對照(分彆為24.0%和20.3%,P＜0.01).EphB4和ephrinB2的錶達在腫瘤細胞中有一緻性,在腫瘤間質血管中有差異性.EphB4和ephrinB2蛋白錶達彊度與淋巴結轉移均呈低度正相關(r=0.370,P=0.045;r=0.362,P=0.047).31例NSCLC患者BF為(42.3±10.2)ml·100 mg-1·min-1,BV為(9.0±3.9)mL/100 g,PEI為(21.6±8.4)HU.相關分析顯示,在NSCLC腫瘤細胞中,EphB4的錶達彊度分彆與BF和BV呈負相關(r=-0.452,P=0.011;r=-0.408,P=0.023),與PEI無關.ephrinB2的錶達彊度分彆與BF和BV呈正相關(r=0.516,P=0.003;r=0.448,P=0.013),與PEI無關.EphB4錶達彊暘性組和暘性組的BF與BV值均顯著低于EphB4錶達陰性組(均P＜0.01).ephrinB2錶達彊暘性組的BF值顯著高于ephrinB2錶達暘性組和陰性組(均P＜0.01),ephrinB2錶達彊剛性組的BV值顯著高于ephrinB2錶達陰性組(P＜0.01).結論 灌註成像反映功能性管腔化血管的密度差異,這種差異同時取決于管腔化血管的數量與質量.
목적 탐토비소세포폐암(NSCLC)조직중EphB4화ephrinB2적표체급기여CT폐관주성상적관계.방법 대31례NSCLC환자적폐내단발결절행CT관주성상,획득혈류량(BF)、혈용량(BV)화최고증강치(PEI)수거.채용면역조화SP법검측EphB4화ephrinB2재종류세포여간질혈관중적표체.운용Spearman상관분석여추세검험탐토EphB4화ephrinB2단백표체여NSCLC림상병리특정、관주삼수적관계.결과 31례NSCLC조직중,22례(71.0%)EphE4표체양성,26례(83.9%)ephrinB2표체양성,명현고우내대조(분별위24.0%화20.3%,P＜0.01).EphB4화ephrinB2적표체재종류세포중유일치성,재종류간질혈관중유차이성.EphB4화ephrinB2단백표체강도여림파결전이균정저도정상관(r=0.370,P=0.045;r=0.362,P=0.047).31례NSCLC환자BF위(42.3±10.2)ml·100 mg-1·min-1,BV위(9.0±3.9)mL/100 g,PEI위(21.6±8.4)HU.상관분석현시,재NSCLC종류세포중,EphB4적표체강도분별여BF화BV정부상관(r=-0.452,P=0.011;r=-0.408,P=0.023),여PEI무관.ephrinB2적표체강도분별여BF화BV정정상관(r=0.516,P=0.003;r=0.448,P=0.013),여PEI무관.EphB4표체강양성조화양성조적BF여BV치균현저저우EphB4표체음성조(균P＜0.01).ephrinB2표체강양성조적BF치현저고우ephrinB2표체양성조화음성조(균P＜0.01),ephrinB2표체강강성조적BV치현저고우ephrinB2표체음성조(P＜0.01).결론 관주성상반영공능성관강화혈관적밀도차이,저충차이동시취결우관강화혈관적수량여질량.
Objective To investigate the role of the expression of ephrinB2 and EphB4 in non-small cell lung cancer (NSCLC), and their relationship with multi-slice spiral CT pulmonary perfusion imaging.Methods Thirty-one nodules with pathologically proven NSCLC underwent CT perfusion scan. The perfusion parameters including blood flow (BF) , blood volume (BV), peak enhancement image (PEI)were collected. The expression of ephrinB2 and EphB4 in tumor cells and interstitial vasculature were detected by immunohistochemistry. Correlation analysis and trend test were used to assess the relationship between ephrinB2/EphB4 expression and clinicopathological features, and between ephrinB2/EphB4 expression and perfusion parameters. Results Positive expression of ephrinB2 and EphB4 in the NSCLC group was 83.9%and 71.0%, respectively, significantly higher than that in the internal control group ( P ＜ 0.01 ). The expression of ephrinB2 and EphB4 was consistently in tumor parenchyma but differently in tumor vessels.The expressions of ephrinB2 and EphB4 were positively correlated with lymphatic metastasis ( P ＜ 0. 05 ).The expression of EphB4 was negatively correlated with blood flow (BF) and blood volume (BV),respectively (P ＜ 0.05 ). There was a significant positive correlation between ephrinB2 expression and BF (r =0.516,P =0. 003), and a positive correlation between ephrinB2 expression and BV (r = 0.448, P =0.013 ). The expressions of ephrinB2 and EphB4 were not correlated with PEI (P ＞0.05 ). The values of BF and BV in the high and moderate EphB4 expression groups were significantly decreased compared with that in the negative group ( P ＜ 0. 01 ). The value of BF in the high ephrinB2 expression group was significantly increased compared with that in the moderately positive group and negative group (P ＜0.01 ).The value of BV in the high ephrinB2 expression group was significantly increased compared with that in the negative group (P ＜ 0. 01 ). Conclusion The CT pulmonary perfusion imaging reflects the density difference of blood vessels with functional lumen, and such difference also depends on the quantity and quality of vasculature with functional lumen.
