CAJ | 학술논문

[目的]了解裂叶牵牛花色、叶色等变化机理。[方法]通过田间调查,利用PCR技术从裂叶牵牛蓝白相间突变株幼叶组织中扩增出目的片段,构建重组质粒pMD18-T/Tpn。[结果]田间调查结果发现,变异株的F3代发生分离,叶色突变并不影响花色的变化;PCR扩增结果表明,以蓝白、纯蓝、大花基因组DNA在330bp处各有1条特异带,而春光、白雪、平安红、垂蔓都没有特异带出现。[结论]变异株重组质粒序列为裂叶牵牛的转座子序列片段。
[목적]료해렬협견우화색、협색등변화궤리。[방법]통과전간조사,이용PCR기술종렬협견우람백상간돌변주유협조직중확증출목적편단,구건중조질립pMD18-T/Tpn。[결과]전간조사결과발현,변이주적F3대발생분리,협색돌변병불영향화색적변화;PCR확증결과표명,이람백、순람、대화기인조DNA재330bp처각유1조특이대,이춘광、백설、평안홍、수만도몰유특이대출현。[결론]변이주중조질립서렬위렬협견우적전좌자서렬편단。
[Objective]The research aimed at investigating mechanisms of the changes of pharbitis.nil flower colors and leaf colors.[Method]The recombinant plasmid pMD18-T/Tpn was constructed by amplifying the target fragments from young leaf tissues of the blue and white mutant of Pharbitis nil with PCR technology and investigating in the fields.[Result]The results of investigations in the fields suggested that the mutant was segregated in F3 generation,and the mutations of leaf colors did not affect the changes of flower colors.The results of PCR amplification showed that there was a band about 330 bp in genomic DNA of Lanbai,Chunlan,Dahua,respectively,however,no bands were found in genomic DNA of Chunguang,Baixue,Ping＇anhong and Chuiman.[Conclusion]The sequence of recombinant plasmid of the mutant was indeed a fragment of the transposon of Pharbitis nil.