国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2008年
5期
295-297
,共3页
洪楷%丁映淑%林美珊%邱小华
洪楷%丁映淑%林美珊%邱小華
홍해%정영숙%림미산%구소화
肝炎,乙型%前S1抗原%病毒%复制
肝炎,乙型%前S1抗原%病毒%複製
간염,을형%전S1항원%병독%복제
Hepatitis B%Pre-S1 antigen%Virus%Replication
目的 了解乙型肝炎患者前S1抗原(Pre-S1)、HBV DNA及HBeAg的相关性,并评价它们在诊断HBV复制中的临床意义.方法 应用ELISA、荧光定量PCR法(FQ-PCR)对198例乙型肝炎患者进行Pre-S1、HBV DNA及HBV血清标志物五项(两对半)检测.结果 198例乙型肝炎患者中Pre-S1阳性107例(54.0%),HBV DNA阳性111例(56.1%),HBeAg阳性90例(45.5%),三者同时阳性72例(36.4%),Pre-S1与HBV DNA阳性率比较,差异无统计学意义(P>0.05).在90例HBeAg阳性样本中,Pre-S1阳性72例(80.0%),HBVDNA阳性74例(82.2%),三者具有较高的符合率.在108例HBeAg阴性样本中,Pre-S1阳性35例(32.4%),HBV DNA阳性37例(34.2%),Pre-S1与HBV DNA检出率比较,两者高度一致(χ2=0.08,P>0.05).结论 Pre-S1与HBV DNA能敏感反映HBV复制的情况,尤其可以协助诊断HBeAg阴性的乙型肝炎患者是否有HBV复制,对于判断患者的病情及指导治疗具有重要的临床意义.
目的 瞭解乙型肝炎患者前S1抗原(Pre-S1)、HBV DNA及HBeAg的相關性,併評價它們在診斷HBV複製中的臨床意義.方法 應用ELISA、熒光定量PCR法(FQ-PCR)對198例乙型肝炎患者進行Pre-S1、HBV DNA及HBV血清標誌物五項(兩對半)檢測.結果 198例乙型肝炎患者中Pre-S1暘性107例(54.0%),HBV DNA暘性111例(56.1%),HBeAg暘性90例(45.5%),三者同時暘性72例(36.4%),Pre-S1與HBV DNA暘性率比較,差異無統計學意義(P>0.05).在90例HBeAg暘性樣本中,Pre-S1暘性72例(80.0%),HBVDNA暘性74例(82.2%),三者具有較高的符閤率.在108例HBeAg陰性樣本中,Pre-S1暘性35例(32.4%),HBV DNA暘性37例(34.2%),Pre-S1與HBV DNA檢齣率比較,兩者高度一緻(χ2=0.08,P>0.05).結論 Pre-S1與HBV DNA能敏感反映HBV複製的情況,尤其可以協助診斷HBeAg陰性的乙型肝炎患者是否有HBV複製,對于判斷患者的病情及指導治療具有重要的臨床意義.
목적 료해을형간염환자전S1항원(Pre-S1)、HBV DNA급HBeAg적상관성,병평개타문재진단HBV복제중적림상의의.방법 응용ELISA、형광정량PCR법(FQ-PCR)대198례을형간염환자진행Pre-S1、HBV DNA급HBV혈청표지물오항(량대반)검측.결과 198례을형간염환자중Pre-S1양성107례(54.0%),HBV DNA양성111례(56.1%),HBeAg양성90례(45.5%),삼자동시양성72례(36.4%),Pre-S1여HBV DNA양성솔비교,차이무통계학의의(P>0.05).재90례HBeAg양성양본중,Pre-S1양성72례(80.0%),HBVDNA양성74례(82.2%),삼자구유교고적부합솔.재108례HBeAg음성양본중,Pre-S1양성35례(32.4%),HBV DNA양성37례(34.2%),Pre-S1여HBV DNA검출솔비교,량자고도일치(χ2=0.08,P>0.05).결론 Pre-S1여HBV DNA능민감반영HBV복제적정황,우기가이협조진단HBeAg음성적을형간염환자시부유HBV복제,대우판단환자적병정급지도치료구유중요적림상의의.
Objective To study the associations among Pre-S1 antigen(Pre-S1), HBV DNA and HBeAg and explore the significance of HBV Pre-S1, HBV DNA, HBeAg detection in the viral replication. Method Pre-S1, serum markers of HBV and HBV DNA concentrations of 198 sere of hepatitis B patients were de,ted by ELISA and FQ-PCR. Results The positive rates of Pre-S1, HBV DNA and HBeAg were 54.0% (107/198), 56.1% (111/198) and 45.5%(90/198) respectively among 198 patients . The same positive rates of them were 36.4%(72/198). In 90 patients with HBeAg positive, the positive rates of Pre-S1 and HBV DNA were 80% (72/90) ,82.2% (74/171) respectively,the coincidence rates of them were great ( P > 0.05). In 108 patients with HBeAg negative, the positive rates of Pre-S1 and HBV DNA were 32.4% (35/108),34.2% (37/108) respectively. There was no significant difference in positive rate between Pre-S1 and HBV DNA( χ2 = 0.08, P > 0.05). Conclusions Pre-S1 and HBV DNA can reflect the replication of HBV sensitively, especially for those who are HBeAg negative, and can be used as a helpful tool for the diagnosis of HBV infection and clinical evaluation.
