CAJ | 학술논문

目的对食管鳞癌癌周亚临床病灶组织和与之相衔接的分化正常的食管组织中MMP-2基因和Survivin基因的表达情况进行检测,为从分子水平研究食管鳞癌的放疗靶区提供参考.方法对34例食管鳞癌患者的癌及癌周组织标本进行连续取材并应用免疫组化法检测MMP-2基因和Survivin基因在癌组织和癌周组织的表达情况,测量两基因在癌周食管组织中的阳性表达范围,并对该范围值与肿瘤分期、肿瘤纵向长度的关系进行分析.结果 MMP-2、Survivin基因在癌组织中阳性表达率分别为85%、76%,在阳性表达标本中癌周亚临床病灶的分别为83%、85%,相邻正常食管组织中的分别为79%、85%;亚临床病灶组织中MMP-2、Survivin基因的阳性表达水平明显高于癌周正常食管组织的表达(χ2=6.46,P=0.028;χ2=16.15,P=0.001).食管上、下两端癌阉食管组织中MMP-2基因的阳性表达范围为17.2～70.4 mm和15.0～82.4 mm,其中97%以上患者的表达范围＜70 mm;Survivin基因阳性表达范围为3.7～76.4 am和16.1～56.3 mm,其中96%以上患者的表达范围＜70 mm;两基因在癌周食管组织中的阳性表达范围随肿瘤分期的进展呈增加趋势.随着肿瘤纵向长度的增加两基因在癌周食管组织中的阳性表达纵向范围呈现增加趋势,其中Survivin基因在癌周食管上下端中的阳性表达范围与肿瘤的纵向长度之间存在正相关性(r=0.566、0.416,P=0.003、0.034).结论 MMP-2、Survivin基因在食管癌周组织中存在一定程度和范围阳性表达,其中96%以上患者两基因阳性表达范围＜70 mm且该范围内大小与肿瘤病期进展密切相关,而相邻分化正常食管组织中也存在着阳性表达应给予关注.
목적 대식관린암암주아림상병조조직화여지상함접적분화정상적식관조직중MMP-2기인화Survivin기인적표체정황진행검측,위종분자수평연구식관린암적방료파구제공삼고.방법 대34례식관린암환자적암급암주조직표본진행련속취재병응용면역조화법검측MMP-2기인화Survivin기인재암조직화암주조직적표체정황,측량량기인재암주식관조직중적양성표체범위,병대해범위치여종류분기、종류종향장도적관계진행분석.결과 MMP-2、Survivin기인재암조직중양성표체솔분별위85%、76%,재양성표체표본중암주아림상병조적분별위83%、85%,상린정상식관조직중적분별위79%、85%;아림상병조조직중MMP-2、Survivin기인적양성표체수평명현고우암주정상식관조직적표체(χ2=6.46,P=0.028;χ2=16.15,P=0.001).식관상、하량단암엄식관조직중MMP-2기인적양성표체범위위17.2～70.4 mm화15.0～82.4 mm,기중97%이상환자적표체범위＜70 mm;Survivin기인양성표체범위위3.7～76.4 am화16.1～56.3 mm,기중96%이상환자적표체범위＜70 mm;량기인재암주식관조직중적양성표체범위수종류분기적진전정증가추세.수착종류종향장도적증가량기인재암주식관조직중적양성표체종향범위정현증가추세,기중Survivin기인재암주식관상하단중적양성표체범위여종류적종향장도지간존재정상관성(r=0.566、0.416,P=0.003、0.034).결론 MMP-2、Survivin기인재식관암주조직중존재일정정도화범위양성표체,기중96%이상환자량기인양성표체범위＜70 mm차해범위내대소여종류병기진전밀절상관,이상린분화정상식관조직중야존재착양성표체응급여관주.
Objective To examine the expression of MMP-2 gene and Survivin gene in subclinical microscopic tumor and its peripheral normal esophageal tissues,and study the radiation target in molecular level. Methods Esophageal squamous cancer and its peripheral tissue samples of 34 patients were cut into sequential sections.The expression of MMP-2 gene and Survivin gene then examined.The length of the peripheral esophageal tissue,positively expressing the two genes,was measured,and the relation among the experimental date,tumor stage and vertical length of tumor were analyzed. Results For tumor tissue,subclinical microscopic tumor and the peripheral differentiated normal tissue,the positive expression rate of MMP-2 was 85%,83%and 79%,respectively.The positive expression rate of Survivin was 76%,85%and 85%,respectively.The positive expression level of both MMP-2 and Survivin genes in subclinical microscopic tumor was significantly higher than that in the peripheral differentiated normal tissue(χ2=6.46,P=0.028 and χ2=16.15,P=0.001).The length was 17.2-70.4 mm and 15.0-82.4 mm of cancerous peripheral tissue with positive expression of MMP-2 gene upside and downside of the tumor.The length was＜70 mm in 97% of the samples.For Survivin gene.the length was 3.7-76.4 mm and 16.1-56.3 mm.and was＜70 mm in 96%of the samples.The length of cancerous peripheral esophageal tissue expressing the two genes increased significantly along with tumor stage or tumor length,and there was statistical correlation between the length of tumor and the positive expression ranges of Survivin gene. Conclusions Both MMP-2 gene and Survivin gene are positively expressed in esophageal cancerous peripheral tissue.The range positively expressing the two genes is＜70 mm in more than 96%of the samples,and the length is correlated with the tumor stage.More attention should be paid to the peripheral differentiated normal tissue with positive expression of MMP-2 gene and Survivin gene in esophageal squamous carcinoma.