中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2012年
4期
361-365
,共5页
肖琼%王春艳%田铧%索宁%张肇林%扈燕来%田广平%刘执玉
肖瓊%王春豔%田鏵%索寧%張肇林%扈燕來%田廣平%劉執玉
초경%왕춘염%전화%색저%장조림%호연래%전엄평%류집옥
骨髓%干细胞%细胞黏附分子%内皮细胞%大鼠
骨髓%榦細胞%細胞黏附分子%內皮細胞%大鼠
골수%간세포%세포점부분자%내피세포%대서
Bone marrow%Stem cells%Cell adhesion molecules%Endothelial cells%Rats
目的 探讨TNF -α促进大鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)向局部损伤组织迁移的可行性. 方法 将MSCs用不同浓度的TNF -α刺激,检测其表面黏附分子、干细胞标志物的表达率及其与内皮细胞的黏附；制作大鼠自体血栓微粒,建立后肢缺血模型,选取一合适浓度的TNF -α刺激MSCs,移植到后肢缺血损伤的大鼠,计数损伤处MSCs的数目.结果 (1)TNF-α刺激24h后,MSCs的血管细胞黏附分子-1(VCAM -1)表达升高,且呈浓度依赖性,而细胞黏附分子-1 (ICAM -1)、L-选择素(L- Selectin)、细胞黏附分子缓慢抗原-4(VLA -4)的表达无明显变化；MSCs的标志物表达率没有明显改变；(2)10 ng/ml TNF -α刺激的MSCs与血管内皮细胞黏附能力明显增强；(3)10 ng/ml TNF -α刺激的MSCs移植到大鼠后肢缺血模型后,损伤侧肌组织内MSCs数目明显多于对照组. 结论 10 ng/ml TNF -α短期内能够有效提高MSCs的VCAM -1表达,促进MSCs与血管内皮细胞的黏附及向受损部位迁移,且不影响MSCs的特性.
目的 探討TNF -α促進大鼠骨髓間充質榦細胞(mesenchymal stem cells,MSCs)嚮跼部損傷組織遷移的可行性. 方法 將MSCs用不同濃度的TNF -α刺激,檢測其錶麵黏附分子、榦細胞標誌物的錶達率及其與內皮細胞的黏附；製作大鼠自體血栓微粒,建立後肢缺血模型,選取一閤適濃度的TNF -α刺激MSCs,移植到後肢缺血損傷的大鼠,計數損傷處MSCs的數目.結果 (1)TNF-α刺激24h後,MSCs的血管細胞黏附分子-1(VCAM -1)錶達升高,且呈濃度依賴性,而細胞黏附分子-1 (ICAM -1)、L-選擇素(L- Selectin)、細胞黏附分子緩慢抗原-4(VLA -4)的錶達無明顯變化；MSCs的標誌物錶達率沒有明顯改變；(2)10 ng/ml TNF -α刺激的MSCs與血管內皮細胞黏附能力明顯增彊；(3)10 ng/ml TNF -α刺激的MSCs移植到大鼠後肢缺血模型後,損傷側肌組織內MSCs數目明顯多于對照組. 結論 10 ng/ml TNF -α短期內能夠有效提高MSCs的VCAM -1錶達,促進MSCs與血管內皮細胞的黏附及嚮受損部位遷移,且不影響MSCs的特性.
목적 탐토TNF -α촉진대서골수간충질간세포(mesenchymal stem cells,MSCs)향국부손상조직천이적가행성. 방법 장MSCs용불동농도적TNF -α자격,검측기표면점부분자、간세포표지물적표체솔급기여내피세포적점부；제작대서자체혈전미립,건립후지결혈모형,선취일합괄농도적TNF -α자격MSCs,이식도후지결혈손상적대서,계수손상처MSCs적수목.결과 (1)TNF-α자격24h후,MSCs적혈관세포점부분자-1(VCAM -1)표체승고,차정농도의뢰성,이세포점부분자-1 (ICAM -1)、L-선택소(L- Selectin)、세포점부분자완만항원-4(VLA -4)적표체무명현변화；MSCs적표지물표체솔몰유명현개변；(2)10 ng/ml TNF -α자격적MSCs여혈관내피세포점부능력명현증강；(3)10 ng/ml TNF -α자격적MSCs이식도대서후지결혈모형후,손상측기조직내MSCs수목명현다우대조조. 결론 10 ng/ml TNF -α단기내능구유효제고MSCs적VCAM -1표체,촉진MSCs여혈관내피세포적점부급향수손부위천이,차불영향MSCs적특성.
Objective To study the feasibility of TNF-α promoting migration of rat mesenchymal stem cells (MSCs) to local damaged tissues. Methods The MSCs was exposed to TNF-α at different concentrations and the expression rate of surface adheslon molecules and specific markers as well as their adhesion to endothelial cells were detected.Based on the above steps,the MSCs stimulated with the optimal concentration of TNF-α were obtained and were injected intravenously to the rats whose hindlimbs experienced ischemia damage.The rats were executed for achieving the muscle samples in the ischemic area,which were made into frozen section to count the number of MSCs. Results ( 1 ) Twenty-four hours after the TNF-o stimulation,the expression of adhesion molecule (VCAM-1) of MSCs increased in a concentration-dependent manner,while the expression of adhesion molecules (ICAM-1,L-Selectin and VLA4) of MSCs showed no significant changes.Besides,the expression rate of specific markers of MSCs was also obscure.(2) Exposed to 10 ng/ml TNF-o,MSCs presented an obviously increased ability in adhesion to the endothelial cells.(3) MSCs stimulated with 10 ng/ml TNF-α showed a larger number in the ischemia-damaged tissue of rat hindlimbs than that in the control group. Conclusion TNF-α at concentration of 10 ng/ml is effective within a short term in increasing VCAM-1 expression in rat MSCs and promoting the adhesion of MSCs to endothelial cells without affecting their character.
