中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2010年
4期
345-349,353
,共6页
张发兵%柯以铨%姜晓丹%胡昌辰%孙新林%卢建侃%杜谋选
張髮兵%柯以銓%薑曉丹%鬍昌辰%孫新林%盧建侃%杜謀選
장발병%가이전%강효단%호창신%손신림%로건간%두모선
NADPH氧化酶%活性氧%DPI%神经胶质瘤%细胞凋亡
NADPH氧化酶%活性氧%DPI%神經膠質瘤%細胞凋亡
NADPH양화매%활성양%DPI%신경효질류%세포조망
NADPH oxidase%Reactive oxygen species%Diphenyleneiodonium%Glioma%Apoptosis
目的 研究NADPH氧化酶(NOX)对U251胶质瘤细胞存活、增殖和凋亡的影响.方法 RT-PCR检测U251胶质瘤细胞系NOX基因的表达.分别使用5、15、25 μmol/LNOX抑制剂DPI及10 mmol/L抗氧化剂Tiron处理U251细胞,24h后alamarBlue法检测U251细胞的增殖,流式细胞术检测细胞内活性氧的产生和U251细胞的凋亡情况,并与正常对照组(不做任何处理的1的U251细胞进行比较.结果 U251细胞系中明显表达NOX4 mRNA.各浓度DPI及10 mmol/Ltiron均能抑制U251胶质瘤细胞的生长,诱导U251细胞凋亡.与正常对照组比较,各浓度DPI处理组的U251细胞内的活性氧簇(ROS)均明显减少,差异有统计学意义(P<0.05); 结论 NOX4可能是胶质瘤细胞内ROS生产的主要来源.NOX4可能通过增加细胞内ROS水平并作用于其下游调节分子,对胶质瘤细胞的生长、存活和凋亡起着重要的调节作用.
目的 研究NADPH氧化酶(NOX)對U251膠質瘤細胞存活、增殖和凋亡的影響.方法 RT-PCR檢測U251膠質瘤細胞繫NOX基因的錶達.分彆使用5、15、25 μmol/LNOX抑製劑DPI及10 mmol/L抗氧化劑Tiron處理U251細胞,24h後alamarBlue法檢測U251細胞的增殖,流式細胞術檢測細胞內活性氧的產生和U251細胞的凋亡情況,併與正常對照組(不做任何處理的1的U251細胞進行比較.結果 U251細胞繫中明顯錶達NOX4 mRNA.各濃度DPI及10 mmol/Ltiron均能抑製U251膠質瘤細胞的生長,誘導U251細胞凋亡.與正常對照組比較,各濃度DPI處理組的U251細胞內的活性氧簇(ROS)均明顯減少,差異有統計學意義(P<0.05); 結論 NOX4可能是膠質瘤細胞內ROS生產的主要來源.NOX4可能通過增加細胞內ROS水平併作用于其下遊調節分子,對膠質瘤細胞的生長、存活和凋亡起著重要的調節作用.
목적 연구NADPH양화매(NOX)대U251효질류세포존활、증식화조망적영향.방법 RT-PCR검측U251효질류세포계NOX기인적표체.분별사용5、15、25 μmol/LNOX억제제DPI급10 mmol/L항양화제Tiron처리U251세포,24h후alamarBlue법검측U251세포적증식,류식세포술검측세포내활성양적산생화U251세포적조망정황,병여정상대조조(불주임하처리적1적U251세포진행비교.결과 U251세포계중명현표체NOX4 mRNA.각농도DPI급10 mmol/Ltiron균능억제U251효질류세포적생장,유도U251세포조망.여정상대조조비교,각농도DPI처리조적U251세포내적활성양족(ROS)균명현감소,차이유통계학의의(P<0.05); 결론 NOX4가능시효질류세포내ROS생산적주요래원.NOX4가능통과증가세포내ROS수평병작용우기하유조절분자,대효질류세포적생장、존활화조망기착중요적조절작용.
Objective To investigate the effects of NADPH oxidase (NOX) on the survival,proliferation and apoptosis of U251 glioma cells. Methods RT-PCR was employed to examine the expressions of NOX genes in the U251 glioma cells. Five, 15 and 25 μmol/L diphenyleneiodonium (DPI,the NOX inhibitor) and 10 mmol/L antioxidant Tiron were added into the cells, respectively, and 24 h after that, the proliferation of U251 glioma cells was tested by alamarBlue assay, and the production of intraceilular reactive oxygen species (ROS) and the apoptosis of U251 glioma cells were examined by flow cytometry. These results were compared with those in the normal control group. Results High mRNA expression level of NOX4 gene was found in the U251 glioma cells. DPI at each concentration can inhibit the growth of U251 glinma cells and induce the apoptosis of U251 glioma cells. Compared with the normal control group, the treatment groups showed significantly decreased ROS in the U251 glioma cells (P<0.05). Conclusion NOX4 gene may be a major source that generates the intracellular ROS in the glioma cells. NOX4 gene regulates the proliferation, survival and apoptosis of glioma cells through increasing the level of ROS and then acting on its downstream regulatory molecules.