中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2011年
2期
112-113
,共2页
张琳%吴峰%石理兰%窦晓光
張琳%吳峰%石理蘭%竇曉光
장림%오봉%석리란%두효광
肝炎病毒,乙型%DNA%肝炎表面抗原,乙型%肝炎e抗原,乙型
肝炎病毒,乙型%DNA%肝炎錶麵抗原,乙型%肝炎e抗原,乙型
간염병독,을형%DNA%간염표면항원,을형%간염e항원,을형
Hepatitis B%DNA%Hepatitis B surface antigens%Hepatitis B e antigens
目的 探讨HBV携带者血清病毒标志物和HBV DNA与肝组织中HBVcccDNA之间的关系.方法 应用实时荧光定量聚合酶链反应(RT-PCR)方法检测30例经肝组织活检病理检查确定为HBV携带者的肝组织中HBVcccDNA、HBVtDNA和血清HBV DNA,同时用化学发光免疫分析法检测HBsAg、HBeAg定量,分析感染者肝组织内HBVcccDNA与肝组织内HBVtDNA、血清HBVDNA、HBsAg及HBeAg定量水平之间的关系.结果 HBV携带者肝组织中均可检出HBVcccDNA,范围在3.15×103拷贝/mg～1.06×107拷/mg(对数值:5.66±0.93);肝组织cccDNA定量与肝组织总HBV DNA定量呈正相关(r=0.375,P<0.05),与血清HBV DNA无相关性(r=0.174,P>0.05).肝组织中HBVcccDNA水平与血清HBsAg定量呈高度正相关(r=0.562,P<0.001);而与血清HBeAg定量无相关性(r=0.152,P>0.05).结论 HBV携带者肝组织内HBVcccDNA成稳定的中等水平复制;血清HBV DNA载量不能直接代表其肝组织中的HBVcccDNA水平;血清HBsAg定量可作为反映肝组织中HBVcccDNA水平的指标.
目的 探討HBV攜帶者血清病毒標誌物和HBV DNA與肝組織中HBVcccDNA之間的關繫.方法 應用實時熒光定量聚閤酶鏈反應(RT-PCR)方法檢測30例經肝組織活檢病理檢查確定為HBV攜帶者的肝組織中HBVcccDNA、HBVtDNA和血清HBV DNA,同時用化學髮光免疫分析法檢測HBsAg、HBeAg定量,分析感染者肝組織內HBVcccDNA與肝組織內HBVtDNA、血清HBVDNA、HBsAg及HBeAg定量水平之間的關繫.結果 HBV攜帶者肝組織中均可檢齣HBVcccDNA,範圍在3.15×103拷貝/mg～1.06×107拷/mg(對數值:5.66±0.93);肝組織cccDNA定量與肝組織總HBV DNA定量呈正相關(r=0.375,P<0.05),與血清HBV DNA無相關性(r=0.174,P>0.05).肝組織中HBVcccDNA水平與血清HBsAg定量呈高度正相關(r=0.562,P<0.001);而與血清HBeAg定量無相關性(r=0.152,P>0.05).結論 HBV攜帶者肝組織內HBVcccDNA成穩定的中等水平複製;血清HBV DNA載量不能直接代錶其肝組織中的HBVcccDNA水平;血清HBsAg定量可作為反映肝組織中HBVcccDNA水平的指標.
목적 탐토HBV휴대자혈청병독표지물화HBV DNA여간조직중HBVcccDNA지간적관계.방법 응용실시형광정량취합매련반응(RT-PCR)방법검측30례경간조직활검병리검사학정위HBV휴대자적간조직중HBVcccDNA、HBVtDNA화혈청HBV DNA,동시용화학발광면역분석법검측HBsAg、HBeAg정량,분석감염자간조직내HBVcccDNA여간조직내HBVtDNA、혈청HBVDNA、HBsAg급HBeAg정량수평지간적관계.결과 HBV휴대자간조직중균가검출HBVcccDNA,범위재3.15×103고패/mg～1.06×107고/mg(대수치:5.66±0.93);간조직cccDNA정량여간조직총HBV DNA정량정정상관(r=0.375,P<0.05),여혈청HBV DNA무상관성(r=0.174,P>0.05).간조직중HBVcccDNA수평여혈청HBsAg정량정고도정상관(r=0.562,P<0.001);이여혈청HBeAg정량무상관성(r=0.152,P>0.05).결론 HBV휴대자간조직내HBVcccDNA성은정적중등수평복제;혈청HBV DNA재량불능직접대표기간조직중적HBVcccDNA수평;혈청HBsAg정량가작위반영간조직중HBVcccDNA수평적지표.
Objective To investigate the correlation of sera HBV DNA and serological makers with hepatic tissue HBVcccDNA in chronic HBV carriers. Methods Real time fluorescence quantitative polymerase chain reaction (RT-PCR) were used to detect HBV covalently closed circular DNA (cccDNA) and total intrahepatic HBV DNA from 30 needle-biopsy specimens as well as HBV DNA in sera in chronic HBV carriers. Quantification of the HBsAg, HBeAg in sera were quantified using Chemiluminescence immunoassay. Results HBVcccDNA can be detected in chronic HBV carriers, which rang from 3.15 × 103 copies/mg to 1.06 × 107 copies/mg. There was a positive correlation between the cccDNA and HBVtDNA (r =0. 375, P < 0. 05 ), but there was no correlation between the cccDNA and sera HBV DNA (P =0. 174). There was a positive correlation between cccDNA and sera HBsAg quantification (r =0. 562, P <0. 001 ) but no correlation with sera HBeAg qantification ( r = 0. 152, P > 0. 05 ). Conclusion HBV cccDNA can be replicated stably in hepatic tissue in all chronic HBV carriers. HBV DNA in sera can not be indicated hepatic tissue cccDNA level. While HBsAg quantification in sera can be used as a marker of cccDNA quantification in hepatic tissue to some extent.
