CAJ | 학술논문

目的对表达HPV16L1抗原的重组腺病毒及1型重组AAV载体联合免疫效果进行研究.方法分别构建含密码子优化型HPVl6LI基因重组腺病毒rAd-mod.HPV16L1及1型重组从V载体rAAV1-mod-HPV 16L1,将纯化的重组AAV病毒载体以肌注及滴鼻途径单独及联合免疫C57BL/6小鼠,使用体外中和实验检测各组小鼠血清中特异性中和抗体.结果 rAAV1-med-HPV16L1单独及与rAd-mod-HPV16L1联合肌注可诱导高滴度的血清中和抗体,在初免后第16周抗体滴度显著高于其他免疫组,联合肌注组诱导的抗体滴度高于单独肌注组;重组病毒联合滴鼻虽能产生一定的免疫加强作用,但抗体滴度仍显著低于rAAV1-mod-HPV16L1单独及联合肌注组.结论型重组从V载体联合重组腺病毒以初免.加强模式肌注可诱导更高滴度的血清中和抗体.
목적 대표체HPV16L1항원적중조선병독급1형중조AAV재체연합면역효과진행연구.방법 분별구건함밀마자우화형HPVl6LI기인중조선병독rAd-mod.HPV16L1급1형중조종V재체rAAV1-mod-HPV 16L1,장순화적중조AAV병독재체이기주급적비도경단독급연합면역C57BL/6소서,사용체외중화실험검측각조소서혈청중특이성중화항체.결과 rAAV1-med-HPV16L1단독급여rAd-mod-HPV16L1연합기주가유도고적도적혈청중화항체,재초면후제16주항체적도현저고우기타면역조,연합기주조유도적항체적도고우단독기주조;중조병독연합적비수능산생일정적면역가강작용,단항체적도잉현저저우rAAV1-mod-HPV16L1단독급연합기주조.결론 형중조종V재체연합중조선병독이초면.가강모식기주가유도경고적도적혈청중화항체.
Objective To evaluate the immune potency of recombinant adenovirus combined with rAAV1 vector expressing HPV16L1 protein in mice. Methods The rAdV and rAAV1 vector containing cedon-medified HPV16L1 gene was constructed using Admax and AAVmax packaging system respectively. C57 BL/6 mice were immunized with purified rAdV and rAAV1 vector through intramuscular and intranasal inoculation routes,and the titer of neutralizing antibody was determined by neutralization assay based HPV16 pseudovirus. Results Intramuscular immunization by rAAV1-med. HPV16L1 or combined with tAd-reed. HPV16L1 can induce higher titer of neutralizing antibody in serum than that of other groups. The titer of neutralizing antibody of intranasal groups is significantly lower than that of intramuscular group,although the prime-boost atrategy using in intranasal group was effective to enhance the specific humoral immunity. Conclusion The rAAV1-med. HPV16L1 combined with tAd-reed. HPV16L1 can induce higher titer of neutralizing antibody in serum through intramuscular route than that of other groups at the 16th week after the first immunization.