CAJ | 학술논문

目的观察体外人脂肪来源干细胞(ADSCs)与Ⅰ型胶原支架的生物相容性.方法 0.125％Ⅰ型胶原酶体外分离人ADSCs,按每只培养瓶1×104个细胞/ml接种,80％融合时进行传代,培养至第3代,将其与Ⅰ型胶原支架体外复合培养(5×105个/cm2胶原支架),细胞计数法检测黏附率,倒置显微镜及扫描电镜观察细胞在支架上黏附生长及增殖.将ADSCs用DⅡ荧光标记并与胶原支架黏附,检测黏附率并与转染前进行比较,检测DII标记后对ADSCs的黏附能力是否有影响.结果体外成功分离得到ADSCs,与Ⅰ型胶原支架能够很好地黏附,在支架上增殖生长状态良好,DⅡ标记前后黏附率分别为(91.9±2.3)％和(90.5±2.0)％,两样本t检验比较差异无统计学意义(P＞0.05).结论 ADSCs与Ⅰ型胶原支架具有良好的生物相容性,Ⅰ型胶原支架可作为脂肪组织工程较理想的生物支架材料.
목적 관찰체외인지방래원간세포(ADSCs)여Ⅰ형효원지가적생물상용성.방법 0.125％Ⅰ형효원매체외분리인ADSCs,안매지배양병1×104개세포/ml접충,80％융합시진행전대,배양지제3대,장기여Ⅰ형효원지가체외복합배양(5×105개/cm2효원지가),세포계수법검측점부솔,도치현미경급소묘전경관찰세포재지가상점부생장급증식.장ADSCs용DⅡ형광표기병여효원지가점부,검측점부솔병여전염전진행비교,검측DII표기후대ADSCs적점부능력시부유영향.결과 체외성공분리득도ADSCs,여Ⅰ형효원지가능구흔호지점부,재지가상증식생장상태량호,DⅡ표기전후점부솔분별위(91.9±2.3)％화(90.5±2.0)％,량양본t검험비교차이무통계학의의(P＞0.05).결론 ADSCs여Ⅰ형효원지가구유량호적생물상용성,Ⅰ형효원지가가작위지방조직공정교이상적생물지가재료.
Objective To study the cellular compatibility of type Ⅰ collagen scaffold and human adipose-derived stem cells (ADSCs).Methods Human ADSCs were isolated with 0.125％ collagenase type Ⅰ and cultured at a density of 1 × 104/ml cells/culture bottle.At the passage 3,ADSCs was co-cultured with type Ⅰ collagen scaffold (5 × 105/cm2 collagen scaffold).The adhesion rate of the ADSCs was determined by cell count method.The morphological features and function of the ADSCs were observed under the invert optical microscopy and scanning electron microscopy.ADSCs were labeled by DⅡ,and bound to type Ⅰ collagen scaffold.The adhesion rate of the ADSCs was compared before and after transfection.Results ADSCs could attach,grow and proliferate well on the scaffolds.Adhesion rate of ADSCs before and after DⅡ labeling was (91.9 ± 2.3 ) ％ and (90.5 ± 2.0) ％ respectively ( P ＞ 0.05 ).Conclusion Collagen Ⅰ scaffold and ADSCs exhibit excellent cellular compatibility and have a bright future in clinical practice.