上海医学
上海醫學
상해의학
SHANGHAI MEDICAL JOURNAL
2009年
12期
1104-1106,后插9
,共4页
于华鹏%王德辉%孙希才%胡俐
于華鵬%王德輝%孫希纔%鬍俐
우화붕%왕덕휘%손희재%호리
内翻性乳头状瘤%癌变%微血管面数密度%血管内皮生长因子%血管生成%CD105
內翻性乳頭狀瘤%癌變%微血管麵數密度%血管內皮生長因子%血管生成%CD105
내번성유두상류%암변%미혈관면수밀도%혈관내피생장인자%혈관생성%CD105
Inverted papilloma%Malignant transformation%Microvessel number of per unit area%Vascularendothelial growth factor%Angiogenesis%CD105
目的 探讨血管内皮生长因子(VEGF)-A_(165)及血管生成在鼻内翻性乳头状瘤(NIP)癌变中的临床意义,评价VEGF-A_(165)与CD105标记的微血管面数密度(MVNA)的相关性.方法 检测CD105免疫荧光标记的正常对照组(9例)、NIP组(23例)及NIP癌变组(8例)的MVNA,应用Western印迹法检测VEGF-A_(165)的表达,采用Spearman相关系数对VEGF-A_(165)蛋白表达量与CD105一MVNA的相关性进行分析.结果 正常对照组、NIP组、NIP癌变组的MVNA依次增高,分别为(33.91±17.88)、(123.35±26.02)、(171.86±49.37)个/mm~2,3组间差异均有统计学意义(P值均<0.01);VEGF-A_(165)蛋白表达依次增高,分别为(94 927.9±8 633.5)、(110 653.2±8 419.7)、(11 6357.1±3 926.5)灰度值/mm~2,3组间的差异均有统计学意义(P值均<0.05).VEGF-AA_(165)与CD105标记的MVNA呈明显正相关(r=0.735,P<0.05).结论 血管生成在NIP癌变中有重要的作用,VEGF-A_(165)与CD105标记的肿瘤MVNA呈正相关,是NIP癌变的预示指标,能为NIP的临床治疗提供参考.
目的 探討血管內皮生長因子(VEGF)-A_(165)及血管生成在鼻內翻性乳頭狀瘤(NIP)癌變中的臨床意義,評價VEGF-A_(165)與CD105標記的微血管麵數密度(MVNA)的相關性.方法 檢測CD105免疫熒光標記的正常對照組(9例)、NIP組(23例)及NIP癌變組(8例)的MVNA,應用Western印跡法檢測VEGF-A_(165)的錶達,採用Spearman相關繫數對VEGF-A_(165)蛋白錶達量與CD105一MVNA的相關性進行分析.結果 正常對照組、NIP組、NIP癌變組的MVNA依次增高,分彆為(33.91±17.88)、(123.35±26.02)、(171.86±49.37)箇/mm~2,3組間差異均有統計學意義(P值均<0.01);VEGF-A_(165)蛋白錶達依次增高,分彆為(94 927.9±8 633.5)、(110 653.2±8 419.7)、(11 6357.1±3 926.5)灰度值/mm~2,3組間的差異均有統計學意義(P值均<0.05).VEGF-AA_(165)與CD105標記的MVNA呈明顯正相關(r=0.735,P<0.05).結論 血管生成在NIP癌變中有重要的作用,VEGF-A_(165)與CD105標記的腫瘤MVNA呈正相關,是NIP癌變的預示指標,能為NIP的臨床治療提供參攷.
목적 탐토혈관내피생장인자(VEGF)-A_(165)급혈관생성재비내번성유두상류(NIP)암변중적림상의의,평개VEGF-A_(165)여CD105표기적미혈관면수밀도(MVNA)적상관성.방법 검측CD105면역형광표기적정상대조조(9례)、NIP조(23례)급NIP암변조(8례)적MVNA,응용Western인적법검측VEGF-A_(165)적표체,채용Spearman상관계수대VEGF-A_(165)단백표체량여CD105일MVNA적상관성진행분석.결과 정상대조조、NIP조、NIP암변조적MVNA의차증고,분별위(33.91±17.88)、(123.35±26.02)、(171.86±49.37)개/mm~2,3조간차이균유통계학의의(P치균<0.01);VEGF-A_(165)단백표체의차증고,분별위(94 927.9±8 633.5)、(110 653.2±8 419.7)、(11 6357.1±3 926.5)회도치/mm~2,3조간적차이균유통계학의의(P치균<0.05).VEGF-AA_(165)여CD105표기적MVNA정명현정상관(r=0.735,P<0.05).결론 혈관생성재NIP암변중유중요적작용,VEGF-A_(165)여CD105표기적종류MVNA정정상관,시NIP암변적예시지표,능위NIP적림상치료제공삼고.
Objective To assess the clinical significance of vascular endothelial growth factor (VEGF)-A_(165), angiogenesis in malignant transformation of nasal inverted papilloma(NIP), and to evaluate the correlation between VEGF-A_(165) and microvessel number of per unit area (MVNA) labeled by CD105. Methods Frozen sections of patients with normal mucosa (9 cases), NIP (23 cases), and squmamous cell carcinoma (SCC) derived from NIP (8 cases) were studied. Serial sections were stained with CD105 by immunofluorescence, and MVNA values were counted. Western blot analysis was used to measure the expression of VEGF-A_(165) The correlation between VEGF-A_(165) and MVNA value was studied. Results The MVNA values was 33.91±17.38 in normal mucosa group, 123.35±26.02 in NIP group, and 171.86±49.37 in SCC group, and there were significant differences between the three groups (P<0.01). VEGF-A_(165)expression densities (absorbance per mm~2 in the normal, NIP, and SCC groups were 94 927.9±8 633.5, 110 653.2±8 419.7, and 116 357.1±3 926.5, respectively, with significant differences found between the three groups (P<0.05). VEGF-A_(165) was positively correlated with CD105-labelled MVNA, (r = 0.735, P<0.05). Conclusion Angiogenesis plays an important role in NIP malignant transformation. VEGF-A_(165) is positively correlated with CD105-1abelled MVNA value. VEGF-A_(165) and CD105-1abelled MVNA value may serve as important indicators to evaluate NIP malignant transformation.
