CAJ | 학술논문

目的探讨色素上皮衍生因子(PEDF)基因在结直肠癌组织中的表达及其与临床病理特征之间的关系.方法采用荧光定量PCR(FQ-PCR)方法检测59例结直肠癌组织及相应癌旁组织中PEDF mRNA的表达水平.结果 59例结直肠癌组织及相应癌旁组织中均可检测出PEDF mRNA的表达,结直肠癌组织中PEDF的表达量为(5.64±0.66)拷贝数/μg,癌旁组织中PEDF的表达量为(6.88±0.67)拷贝数/μg(P=0.007).早期(Dukes A、B期)结直肠癌患者和肿瘤分化程度高者PEDF的表达量显著高于晚期(Dukes C、D期,P=0.003)和肿瘤分化程度低者(P=0.010).结论 PEDF mRNA的水平与结直肠癌的形成密切相关,初步结果显示PEDF可能是结直肠癌发生、发展过程中的一种保护因素.
목적 탐토색소상피연생인자(PEDF)기인재결직장암조직중적표체급기여림상병리특정지간적관계.방법 채용형광정량PCR(FQ-PCR)방법검측59례결직장암조직급상응암방조직중PEDF mRNA적표체수평.결과 59례결직장암조직급상응암방조직중균가검측출PEDF mRNA적표체,결직장암조직중PEDF적표체량위(5.64±0.66)고패수/μg,암방조직중PEDF적표체량위(6.88±0.67)고패수/μg(P=0.007).조기(Dukes A、B기)결직장암환자화종류분화정도고자PEDF적표체량현저고우만기(Dukes C、D기,P=0.003)화종류분화정도저자(P=0.010).결론 PEDF mRNA적수평여결직장암적형성밀절상관,초보결과현시PEDF가능시결직장암발생、발전과정중적일충보호인소.
Objective To study the expression of pigment epithelium-derived factor PEDF gene in colorectal cancer(CRC) and to investigate the relationship between the expression of PEDF mRNA and the clinic pathologicaI features of CRC.Methods The expression of PEDF mRNA in 59 samples of CRC tissues and matched par cancerous colorectal tissues was detected by FQRT-PCR.Results All the CRC tissues and the matched par cancerous colorectal tissues had expression of PEDF mRNA.The level of PEDF mRNA in CRC was(5.64±0.66)(1g(copies)/μg RNA),and(6.88±0.67)(1g(copies)/μg RNA)in the matched par cancerous colorectal tissues respectively(P=0.007).PEDF expression was significantly related to Dukes stages and the tumor differentiated degree.Conclusion The expression of PEDF mRNA is remark-ably correlated with the occurrence of CRC.The primary results show that PEDF may be a protective factor in the formation and progression of CRC.