CAJ | 학술논문

保护地连作是根结线虫为害严重的主要原因,为评估连作年限对根结线虫数量及其与土壤线虫数量关系,选择典型的不同连作时间的保护地(种植番茄),研究番茄生长期间根围土壤不同土层的根结线虫二龄幼虫(second-stage juveniles,J2)与自由生活线虫数量的动态变化.结果表明:根结线虫J2数量随连作年限增加而增多,0和5-year土壤中根结线虫数量少,平均根结线虫数为1.1和2.1条/100 g干土,8、10和12-year土壤中根结线虫数量平均值分别为154.9、68.3和861.8@r/100 g干土;在0-30 cm土层,根结线虫J2数量随土层加深而增加,主要分布在20～30 cm土层.土壤自由生活线虫数量随土层的增加而增多,连作对自由生活线虫数量无显著影响.0和5-year土壤中根结线虫群体占土壤线虫总数的比例小,属稀有类群,在土壤线虫中处于稳定的群落结构状态.连作8、10和12-year的土壤中根结线虫J2为优势类群,土壤中自由生活线虫数量与根结线虫数量呈现相反的趋势,根结线虫J2占土壤线虫总数的比例增加,自由生活线虫与根结线虫J2数量的比值显著降低(P＜0.05).
보호지련작시근결선충위해엄중적주요원인,위평고련작년한대근결선충수량급기여토양선충수량관계,선택전형적불동련작시간적보호지(충식번가),연구번가생장기간근위토양불동토층적근결선충이령유충(second-stage juveniles,J2)여자유생활선충수량적동태변화.결과표명:근결선충J2수량수련작년한증가이증다,0화5-year토양중근결선충수량소,평균근결선충수위1.1화2.1조/100 g간토,8、10화12-year토양중근결선충수량평균치분별위154.9、68.3화861.8@r/100 g간토;재0-30 cm토층,근결선충J2수량수토층가심이증가,주요분포재20～30 cm토층.토양자유생활선충수량수토층적증가이증다,련작대자유생활선충수량무현저영향.0화5-year토양중근결선충군체점토양선충총수적비례소,속희유류군,재토양선충중처우은정적군락결구상태.련작8、10화12-year적토양중근결선충J2위우세류군,토양중자유생활선충수량여근결선충수량정현상반적추세,근결선충J2점토양선충총수적비례증가,자유생활선충여근결선충J2수량적비치현저강저(P＜0.05).
Root-knot nematode(Meloidogyne sp.)is a destructive pest of vegetable and difficult to be managed,especially in greenhouse of continuous planting tomato.For determining the influence of continuous planting on soil nematodes,the dynamics of second-stage juveniles(RKN J2)of root-knot nematode and freeliving nematode in rhizosphem soil were investigated during the tomato growing season.The individuals of RKN J2 increased with the longer planting years.The number of J2 at 0～30 cm in 0 and 5-year soil was significantly less than that of other tested soil(P＜0.05),the average means were 1.1 and 2.1 per 100 g dry soil.While in 8,10 and 12-year soil,J2 were 154.9,68.3 and 861.8 per 100 g dry soil,respectively.The population of J2 increased with soil depth,and mainly distributed in 20～30 cm soil layer.The number of free-living nematode also increased with depth of soil.There was no relationship between the time of continuous cropping and the number of soil free living nematode.The percentage of RKN J2 in total soil nematode was lower in 0 and 5-year,Meloidogyne sp.was the rare group and kept relative stable population structure with the other soil nematodes.However,RKN J2 in 8,10 and 12-year soil was the dominant group.The adverse trend Was found between the number of free living nematode and RKN J2.the proportion of RKN J2 in total soil nematode increased with the time of continuous planting,and the ratio of free-living nematodes to RKN J2 decreased significantly(P＜0.05).