CAJ | 학술논문

DYT1是拟南芥绒毡层发育过程中重要的转录因子.DYT1的缺失会导致绒毡层相关功能的缺陷.然而,DYT1并不是使绒毡层功能化的充分因素,暗示了DYT1蛋白需要和其他转录因子形成复合物才能发挥功能.利用酵母双杂交技术以DYT1蛋白为诱饵在拟南芥cDNA文库中筛选与DYT1相互作用的蛋白.通过筛选从文库中得到20个阳性克隆,通过BLAST序列比对得到8个候选基因序列,从中鉴定出6种候选基因编码可能的转录因子或者其他调控转录的蛋白因子.其中5个编码调控因子的基因都被证实在花药中表达.更进一步,发现另一个对花药发育所必需的转录因子AMS能与DYT1在酵母体内形成转录复合物.表明这2个蛋白可能形成异源复合物在花药中共同调节下游基因的表达.
DYT1시의남개융전층발육과정중중요적전록인자.DYT1적결실회도치융전층상관공능적결함.연이,DYT1병불시사융전층공능화적충분인소,암시료DYT1단백수요화기타전록인자형성복합물재능발휘공능.이용효모쌍잡교기술이DYT1단백위유이재의남개cDNA문고중사선여DYT1상호작용적단백.통과사선종문고중득도20개양성극륭,통과BLAST서렬비대득도8개후선기인서렬,종중감정출6충후선기인편마가능적전록인자혹자기타조공전록적단백인자.기중5개편마조공인자적기인도피증실재화약중표체.경진일보,발현령일개대화약발육소필수적전록인자AMS능여DYT1재효모체내형성전록복합물.표명저2개단백가능형성이원복합물재화약중공동조절하유기인적표체.
DYT1 is a core transcription factor for Arabidopsis tapetum development. Impairment of DYT1 function causes failure of most tapetum-related functions. However, DYT1 alone is not sufficient for overall tapetum function, suggesting there should be other factors DYT1 works with. Using DYT1 as bait in yeast two-hybrid system, screening for the protein interacting with DYT1 was performed in this report. 20 positive colonies were obtained. Blast sequence comparison acquired 8 candidate sequences, from which 6 candidates were exhibited to encode putative transcription factors or other transcription-regulating factors. 5 of these regulatory genes were confirmed to be expressed in anther. Furthermore we found another anther indispensable transcription factor AMS could interact with DYT1 in yeast, suggesting that these two transcription factors form heterozygous transcription complex to control the expression of downstream genes in anther development.