CAJ | 학술논문

目的探讨氨溴索对胃内容物吸入性肺损伤大鼠动物模型的抗炎保护作用.方法 54只健康Sprague-Dawley雄性大鼠随机分为3组:对照组、损伤组、氨溴索组.观察各组大鼠动脉血氧分压(PaO2)、肺组织湿重/干重比(W/D)、髓过氧化物酶(myeloperoxidase,MPO)活性变化；ELISA法测定血清肿瘤坏死因子α (TNF-α)含量；Western blot方法检测肺组织前B细胞克隆增强因子(pre-B-cell colony-enhancing factor,PBEF)蛋白表达；光镜下观察肺组织结构变化.结果与对照组比较,损伤组各时相点PaO2降低(P＜0.05),肺组织W/D以及MPO活性增加(P＜0.05),血浆中TNF-α含量增高(P＜0.05)；PBEF蛋白表达均增高(P＜0.05)；肺组织出现明显病理组织学损伤.与损伤组比较,氨溴索组各时相点PaO2升高(P＜0.05),肺组织W/D以及MPO活性降低(P＜0.05),TNF-α含量降低(P＜0.05)；PBEF蛋白表达均下降(P＜0.05)；肺组织病理学损伤减轻.结论在大鼠胃内容物吸入性肺损伤模型中,氨溴索可通过抑制肺组织PBEF表达、抑制炎症介质的释放发挥抗炎保护作用.
목적 탐토안추색대위내용물흡입성폐손상대서동물모형적항염보호작용.방법 54지건강Sprague-Dawley웅성대서수궤분위3조:대조조、손상조、안추색조.관찰각조대서동맥혈양분압(PaO2)、폐조직습중/간중비(W/D)、수과양화물매(myeloperoxidase,MPO)활성변화；ELISA법측정혈청종류배사인자α (TNF-α)함량；Western blot방법검측폐조직전B세포극륭증강인자(pre-B-cell colony-enhancing factor,PBEF)단백표체；광경하관찰폐조직결구변화.결과 여대조조비교,손상조각시상점PaO2강저(P＜0.05),폐조직W/D이급MPO활성증가(P＜0.05),혈장중TNF-α함량증고(P＜0.05)；PBEF단백표체균증고(P＜0.05)；폐조직출현명현병리조직학손상.여손상조비교,안추색조각시상점PaO2승고(P＜0.05),폐조직W/D이급MPO활성강저(P＜0.05),TNF-α함량강저(P＜0.05)；PBEF단백표체균하강(P＜0.05)；폐조직병이학손상감경.결론 재대서위내용물흡입성폐손상모형중,안추색가통과억제폐조직PBEF표체、억제염증개질적석방발휘항염보호작용.
Objective To investigate the anti-inflammatory roles of ambroxol in rats' gastric aspiration lung injury.Methods Fifty-four Sprague-Dawley rats were randomly divided into three equal groups:control group,iniury group and ambroxol group.Arterial partial pressure of oxygen (PaO2),wetto-dry weight ratio (W/D) of lung,the activity of myeloperoxidase (MPO) were measured.Tumor necrosis factor-α (TNF-α) of serum were examined by enzyme linked immunosorbent assay.Pre-B-cell colony-enhancing factor (PBEF) was examined by Western blot.Lung pathological change was observed by microscope.Results Compared with control group,PaO2 decreased but W/D of lung,the activity of MPO,TNF-α of serum and PBEF expression in injury group increased (all P ＜0.05).Compared with injury group,PaO2 increased but W/D of lung,the activity of MPO,TNF-α of serum and PBEF expression in ambroxol group decreased (all P ＜0.05).The microscopic structure of the control group was normal and the pathological changes of ambroxol group were milder than those of injury group.Conclusions Ambroxol has anti-inflammatory effects by restraining the activity of PBEF and the releasing of inflammatory cytokines on gastric aspiration lung injury.