国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2012年
1期
14-19
,共6页
FQ-PCR%肺炎支原体%MP-DNA%MP-IgM
FQ-PCR%肺炎支原體%MP-DNA%MP-IgM
FQ-PCR%폐염지원체%MP-DNA%MP-IgM
FQ-PCR%Mycoplasmapneumoniae%MP-DNA%MP-IgM
目的 利用FQ-PCR技术和DIGFA试验对儿童呼吸道感染患者分别检测MP-DNA和MP-IgM,观察分析其在不同病程中的变化规律,探讨FQ-PCR检测MP-DNA和DIGFA检测MP-IgM用于儿童肺炎支原体感染中的诊断价值.方法 入选患儿留取呼吸道分泌物和血清标本,采用FQ-PCR法检测呼吸道分泌物MP-DNA,用胶体金DIGFA法检测患儿血清MP-IgM.入院患儿被检出单一MP-DNA阳性或MP-IgM阳性或两者双阳性者均确诊为MP感染病例,对确诊的病例观察分析病程不同时期MP-DNA和MP-IgM阳性率消长的动态及其变化规律.结果 根据患儿MP-DNA和MP-IgM检出情况,被确诊为肺炎支原体感染的患儿共56例.对这56例患儿进行病程追踪检查分析显示:在病程初期阶段,MP-DNA检出阳性率为82.14％( 46/56),MP-IgM阳性率为35.71％( 20/56)；两种检测阳性率比较,MP-DNA阳性率显著高于MP-IgM阳性率,两者差异有显著性(P＜0.05).在病程中期,MP-DNA检出阳性率下降为64.29％( 36/56),MP-IgM阳性率上升为80.36％( 45/56)；到恢复期,MP-DNA阳性检出率继续下降为1.79％( 1/56),而MP-IgM阳性率在中期升高后又返降为23.21％( 13/56).结论 在肺炎支原体感染患儿不同病程中,存在MP-DNA随病程延长逐渐降低而MP-IgM随病程延长逐渐增高至中期又返折下降的消长规律.FQ-PCR检测MP-DNA对MP感染患儿发病初期就诊者确诊价值较高,而DIGFA检测MP-IgM对发病时间已较长而又初次就诊者诊断意义较大.两种方法联合应用既可在诊断上优势互补,又可用于患儿病情转归预测评估.
目的 利用FQ-PCR技術和DIGFA試驗對兒童呼吸道感染患者分彆檢測MP-DNA和MP-IgM,觀察分析其在不同病程中的變化規律,探討FQ-PCR檢測MP-DNA和DIGFA檢測MP-IgM用于兒童肺炎支原體感染中的診斷價值.方法 入選患兒留取呼吸道分泌物和血清標本,採用FQ-PCR法檢測呼吸道分泌物MP-DNA,用膠體金DIGFA法檢測患兒血清MP-IgM.入院患兒被檢齣單一MP-DNA暘性或MP-IgM暘性或兩者雙暘性者均確診為MP感染病例,對確診的病例觀察分析病程不同時期MP-DNA和MP-IgM暘性率消長的動態及其變化規律.結果 根據患兒MP-DNA和MP-IgM檢齣情況,被確診為肺炎支原體感染的患兒共56例.對這56例患兒進行病程追蹤檢查分析顯示:在病程初期階段,MP-DNA檢齣暘性率為82.14％( 46/56),MP-IgM暘性率為35.71％( 20/56)；兩種檢測暘性率比較,MP-DNA暘性率顯著高于MP-IgM暘性率,兩者差異有顯著性(P＜0.05).在病程中期,MP-DNA檢齣暘性率下降為64.29％( 36/56),MP-IgM暘性率上升為80.36％( 45/56)；到恢複期,MP-DNA暘性檢齣率繼續下降為1.79％( 1/56),而MP-IgM暘性率在中期升高後又返降為23.21％( 13/56).結論 在肺炎支原體感染患兒不同病程中,存在MP-DNA隨病程延長逐漸降低而MP-IgM隨病程延長逐漸增高至中期又返摺下降的消長規律.FQ-PCR檢測MP-DNA對MP感染患兒髮病初期就診者確診價值較高,而DIGFA檢測MP-IgM對髮病時間已較長而又初次就診者診斷意義較大.兩種方法聯閤應用既可在診斷上優勢互補,又可用于患兒病情轉歸預測評估.
목적 이용FQ-PCR기술화DIGFA시험대인동호흡도감염환자분별검측MP-DNA화MP-IgM,관찰분석기재불동병정중적변화규률,탐토FQ-PCR검측MP-DNA화DIGFA검측MP-IgM용우인동폐염지원체감염중적진단개치.방법 입선환인류취호흡도분비물화혈청표본,채용FQ-PCR법검측호흡도분비물MP-DNA,용효체금DIGFA법검측환인혈청MP-IgM.입원환인피검출단일MP-DNA양성혹MP-IgM양성혹량자쌍양성자균학진위MP감염병례,대학진적병례관찰분석병정불동시기MP-DNA화MP-IgM양성솔소장적동태급기변화규률.결과 근거환인MP-DNA화MP-IgM검출정황,피학진위폐염지원체감염적환인공56례.대저56례환인진행병정추종검사분석현시:재병정초기계단,MP-DNA검출양성솔위82.14％( 46/56),MP-IgM양성솔위35.71％( 20/56)；량충검측양성솔비교,MP-DNA양성솔현저고우MP-IgM양성솔,량자차이유현저성(P＜0.05).재병정중기,MP-DNA검출양성솔하강위64.29％( 36/56),MP-IgM양성솔상승위80.36％( 45/56)；도회복기,MP-DNA양성검출솔계속하강위1.79％( 1/56),이MP-IgM양성솔재중기승고후우반강위23.21％( 13/56).결론 재폐염지원체감염환인불동병정중,존재MP-DNA수병정연장축점강저이MP-IgM수병정연장축점증고지중기우반절하강적소장규률.FQ-PCR검측MP-DNA대MP감염환인발병초기취진자학진개치교고,이DIGFA검측MP-IgM대발병시간이교장이우초차취진자진단의의교대.량충방법연합응용기가재진단상우세호보,우가용우환인병정전귀예측평고.
Objective To explore the values of FQ-PCR for detecting MP-DNA and DIGFA for MP-IgM in the diagnosis of mycoplasma pneumoniae( MP ) infection in children.Methods Respiratory secretions and serum samples were collected.MP-DNA in respiratory secretions was detected by FQ-PCR and serum MP-IgM by DIGFA.Children with positive MP-DNA or MP-IgM alone or both MP-DNA and MP-IgM were diagnosed as MP infection.The changes in the positive rates of MP-DNA and MP-IgM during different courses were observed and analyzed.Results 56 children were diagnosed as MP infection.In the early disease course,the positive rate of MP-DNA was 82.14％ ( 46/56 ),and that of MP-IgM was 35.71％ ( 20/56 ); the former was significantly higher than the latter,with a significant difference ( P＜ 0.05 ).In the middle disease course,the positive rate of MP-DNA dropped to 64.29％ ( 36/56 ),while that of MP-IgM increased to 80.36％ ( 45/56 ); In the recovery course,the positive rate of MP-DNA continued to decline to 1.79％ ( 1/56 ),while that of MP-IgM decreased to 23.21％ ( 13/56 ).Conclusions In children with MP infection, MP-DNA is gradually decreased with the prolongation of the disease course,while MP-IgM is increased until to the middle course and then is declined.FQ-PCR for MP-DNA has a greater value in the diagnosis of MP infection at the early stage,while DIGFA for MP-IgM has a diagnostic significance for the children who has a longer disease course and makes a first visit.Combination of two methods is beneficial for the diagnosis and can be used for prediction of the disease outcome.
