白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2012年
1期
50-53
,共4页
王欣%顾霞%哈力达·亚森%郭新红
王訢%顧霞%哈力達·亞森%郭新紅
왕흔%고하%합력체·아삼%곽신홍
淋巴瘤%白细胞介素10%白细胞介素6%免疫组织化学%酶联免疫吸附测定
淋巴瘤%白細胞介素10%白細胞介素6%免疫組織化學%酶聯免疫吸附測定
림파류%백세포개소10%백세포개소6%면역조직화학%매련면역흡부측정
Lymphoma%Interleukin-10%Interleukin-6%Immunohistochemistry%Enzyme-Linked immunosorbent assay
目的 探讨白细胞介素( IL )-10和IL-6在不同类型淋巴瘤组织与血浆中的表达及其意义.方法 收集97例淋巴瘤患者石蜡组织和血浆标本,应用免疫组织化学和酶联免疫吸附试验( ELISA)的方法观察组织与血浆中IL-10和IL-6表达情况.结果 IL-10和IL-6在不同类型淋巴瘤组织中皆呈阳性表达,且各亚型之间差异无统计学意义(x2=0.815,x2=0.542,均P＞0.05)；IL-10和IL-6分别在肿瘤中的巨噬细胞和血管内皮细胞也有不同程度的阳性表达.淋巴瘤患者血浆IL-10和IL-6水平[( 232.57±191.59 )pg/ml、( 80.70±89.68) pg/ml]高于健康对照组[(59.12±68.35) pg/ml、( 45.68±33.66) pg/ml],差异有统计学意义(t=6.968,t=2.896,P＜0.05)；血浆IL-10和IL-6水平随肿瘤细胞表达强度增强而增强( x2=0.815,x2=0.542,P＜ 0.05)；IL-10和IL-6在淋巴瘤细胞表达强度两者呈正相关(rs=0.394,P＜0.05).结论 IL-10和IL-6在淋巴瘤患者淋巴瘤组织和血浆中都有表达,淋巴瘤患者血浆IL-10和IL-6水平高于健康对照组,二者在淋巴瘤的发生、发展中起着一定的作用,可能作为淋巴瘤诊断的辅助指标.
目的 探討白細胞介素( IL )-10和IL-6在不同類型淋巴瘤組織與血漿中的錶達及其意義.方法 收集97例淋巴瘤患者石蠟組織和血漿標本,應用免疫組織化學和酶聯免疫吸附試驗( ELISA)的方法觀察組織與血漿中IL-10和IL-6錶達情況.結果 IL-10和IL-6在不同類型淋巴瘤組織中皆呈暘性錶達,且各亞型之間差異無統計學意義(x2=0.815,x2=0.542,均P＞0.05)；IL-10和IL-6分彆在腫瘤中的巨噬細胞和血管內皮細胞也有不同程度的暘性錶達.淋巴瘤患者血漿IL-10和IL-6水平[( 232.57±191.59 )pg/ml、( 80.70±89.68) pg/ml]高于健康對照組[(59.12±68.35) pg/ml、( 45.68±33.66) pg/ml],差異有統計學意義(t=6.968,t=2.896,P＜0.05)；血漿IL-10和IL-6水平隨腫瘤細胞錶達彊度增彊而增彊( x2=0.815,x2=0.542,P＜ 0.05)；IL-10和IL-6在淋巴瘤細胞錶達彊度兩者呈正相關(rs=0.394,P＜0.05).結論 IL-10和IL-6在淋巴瘤患者淋巴瘤組織和血漿中都有錶達,淋巴瘤患者血漿IL-10和IL-6水平高于健康對照組,二者在淋巴瘤的髮生、髮展中起著一定的作用,可能作為淋巴瘤診斷的輔助指標.
목적 탐토백세포개소( IL )-10화IL-6재불동류형림파류조직여혈장중적표체급기의의.방법 수집97례림파류환자석사조직화혈장표본,응용면역조직화학화매련면역흡부시험( ELISA)적방법관찰조직여혈장중IL-10화IL-6표체정황.결과 IL-10화IL-6재불동류형림파류조직중개정양성표체,차각아형지간차이무통계학의의(x2=0.815,x2=0.542,균P＞0.05)；IL-10화IL-6분별재종류중적거서세포화혈관내피세포야유불동정도적양성표체.림파류환자혈장IL-10화IL-6수평[( 232.57±191.59 )pg/ml、( 80.70±89.68) pg/ml]고우건강대조조[(59.12±68.35) pg/ml、( 45.68±33.66) pg/ml],차이유통계학의의(t=6.968,t=2.896,P＜0.05)；혈장IL-10화IL-6수평수종류세포표체강도증강이증강( x2=0.815,x2=0.542,P＜ 0.05)；IL-10화IL-6재림파류세포표체강도량자정정상관(rs=0.394,P＜0.05).결론 IL-10화IL-6재림파류환자림파류조직화혈장중도유표체,림파류환자혈장IL-10화IL-6수평고우건강대조조,이자재림파류적발생、발전중기착일정적작용,가능작위림파류진단적보조지표.
Objective To investigate the expression of IL-10 and IL-6 in tissue and plasma of subtypes lymphoma and its significance. Methods Immunohistochemical technique and enzyme-linked immunosorbent assay (ELISA) were used to assess the expression of IL-10 and IL-6 in 97 cases paraffin tissue and plasma samples from different subtypes of lymphoma.Results Expression of IL-10 and IL-6 was positive in all lymphoma tissue samples, but there is no significant difference among different subtypes lymphoma (x2 =0.815, x2 =0.542, P ＞ 0.05). Tumor samples were found positive IL-10 and IL-6 expression in macrophages and vascular endothelial cells by immunohistochemistry respectively. Expression of IL-10 and IL-6 in plasma of lymphoma were higher than that in control respectively [(232.57±191.59) pg/ml vs (59.12±68.35) pg/ml,(80.70±89.68) pg/ml vs (45.68±33.66) pg/ml],with significant difference (t =6.968,t =2.896,P ＜0.05).IL-10 and IL-6 plasma levels increased with enhanced expression in lymphoma (x2 =0.815,x2 =0.542,P ＜ 0.05).Which was found to be correlated with each other (rs =0.394,P ＜ 0.05). Conclusion The expression of IL-10 and IL-6 had been found at various degrees in tissues and plasma of lymphoma.IL-10 and IL-6 in plasma of lymphoma were higher than that in controls. These results showed that IL-10 and IL-6 may cooperate in vivo with lymphoma cell proliferation and infiltrate. IL-6 and IL-10 maybe served as useful indicators for diagnosis of lymphoma.
