CAJ | 학술논문

目的研究盐酸左氧氟沙星在大鼠胰腺组织的药代动力学.方法采用微透析技术对大鼠胰腺组织和血液进行同步取样,联合反相高效液相色谱实时检测胰腺组织和血液中盐酸左氧氟沙星的浓度变化,所得药物浓度数据用WinNonlin软件处理.结果大鼠血液中的游离左氧氟沙星浓度在给药后10 min时最高,为(65.23±12.9)μg/ml,胰腺组织在20 min到达峰浓度(30.56±3.22)μg/ml,然后持续下降;给药后20 min时胰腺组织的药物浓度开始高于血清,并持续至100 min,然后下降,两者浓度始终保持接近.血液、胰腺的药时曲线下面积分别为(2914.38±205.73)min·μg~(-1)·ml~(-1)和(2465.11±258.56)min·μg~(-1)·ml~(-1).结论微透析采样技术联合反相高效液相色谱检测方法可以准确客观地反映药物在组织和血液中的药代动力学特点,盐酸左氧氟沙星具有较高的胰腺组织药物浓度.
목적 연구염산좌양불사성재대서이선조직적약대동역학.방법 채용미투석기술대대서이선조직화혈액진행동보취양,연합반상고효액상색보실시검측이선조직화혈액중염산좌양불사성적농도변화,소득약물농도수거용WinNonlin연건처리.결과 대서혈액중적유리좌양불사성농도재급약후10 min시최고,위(65.23±12.9)μg/ml,이선조직재20 min도체봉농도(30.56±3.22)μg/ml,연후지속하강;급약후20 min시이선조직적약물농도개시고우혈청,병지속지100 min,연후하강,량자농도시종보지접근.혈액、이선적약시곡선하면적분별위(2914.38±205.73)min·μg~(-1)·ml~(-1)화(2465.11±258.56)min·μg~(-1)·ml~(-1).결론 미투석채양기술연합반상고효액상색보검측방법가이준학객관지반영약물재조직화혈액중적약대동역학특점,염산좌양불사성구유교고적이선조직약물농도.
Objective To investigate the pharmaeokinetics of levofloxacin in rat's pancreatic tissue. Methods Pancreatic tissue and blood were sampled in vivo by microdialysis simultaneously. The concentrations of levofloxacin in beth blood and tissues were measured by high performance liquid chromatography. All date were analyzed by WinNonlin software. Results The maximum concentration of free levofloxacin in blood and pancreatic tissue were (65.23 ± 12.9) μg/ml at 10min and (30.56±3.22) μg/ml at 20 min, respectively, then beth continuously decreased. Concentration of free levofloxacin in pancreatic tissue was higher than that in blood from 20min to 100min, then returned to similar level. The area under the concentration curve(AUC)of unbound levofloxacin was(2465.11±258.56)min·μg~(-1)·ml~(-1) in pancreas,and (2914.38±205.73)min·μg~(-1)·ml~(-1) in blood.Conclusions Microdialysis with reversed phase high performance liquid chromatography established in this essay could be used to determine the pharmacokinetics of levofloxacin objectively. High concentration of levofloxacin in pancreatic tissue and blood was observed.