CAJ | 학술논문

目的探讨甲泼尼龙冲击治疗对重症系统性红斑狼疮(SEE)T细胞内磷酸化转录激活因子1(STATI)表达和对STATI DNA结合活性的作用.方法 6例重症SLE患者进入本研究.采用蛋白免疫印迹和电泳迁移率实验分别检测甲泼尼龙冲击治疗对SLE T细胞内磷酸化STATI的表达和对STATIDNA结合活性的作用.结果甲泼尼龙冲击治疗后72 h,磷酸化STATI的表达大约被降低30%.甲泼尼龙冲击治疗可降低SLE T细胞内STATI的DNA结合活性和表达量.冲击治疗72 h后,STATI的DNA结合活性被抑制约40%,差异有统计学意义(t=3.058,P<0.05).结论抑制磷酸化STATI表达和STATIDNA结合活性是甲泼尼龙发挥作用的可能机制之一.
목적 탐토갑발니룡충격치료대중증계통성홍반랑창(SEE)T세포내린산화전록격활인자1(STATI)표체화대STATI DNA결합활성적작용.방법 6례중증SLE환자진입본연구.채용단백면역인적화전영천이솔실험분별검측갑발니룡충격치료대SLE T세포내린산화STATI적표체화대STATIDNA결합활성적작용.결과 갑발니룡충격치료후72 h,린산화STATI적표체대약피강저30%.갑발니룡충격치료가강저SLE T세포내STATI적DNA결합활성화표체량.충격치료72 h후,STATI적DNA결합활성피억제약40%,차이유통계학의의(t=3.058,P<0.05).결론 억제린산화STATI표체화STATIDNA결합활성시갑발니룡발휘작용적가능궤제지일.
Objective To investigate the effects of methylprednisolone pulse therapy on the expression of phosphorylated signal transducer and activator of transcription 1 (STATI) and DNA-binding activity of STATI in T cells in patients with severe systemic lupus erythematosus (SLE). Methods Six patients were included. Patients were given 0.5～1 g of methylprednisolone on 3 consecutive days. Western Blotting was conducted to explore the phosphorylated STATI expression and electrophoretic mobility shift assays (EMSA) were carried out to detect the DNA-biding activity of STATI. Results Methylprednisolone pulse therapy decreased phosphorylated STATI expression of T cells from patients with severe SLE. The expression of phosphorylated STATI decreased to about 30% 72 h after the methylprednisolone pulse therapy started (t=2.858, P<0.05). Methylprednisolone pulse therapy down-regulated DNA-biding activity of STATI of T cells in patients with severe SLE. The STATI DNA-biding activity was inhibited to about 40% 72 h after methy-Iprednisolone pulse, therapy started (t=3.058, P<0.05). Conclusion Phosphorylated STATI expression and DNA-binding activity of T cells is markedly decreased in patients after methylprednisolone pulse therapy, suggesting that inhibition of STATI signaling contributes to the clinical efficacy of this agent.