CAJ | 학술논문

目的探讨高压氧治疗对急性创伤性脑损伤(traumatic brain injury,TBI)大鼠神经行为学及细胞外信号调节激酶(extra- cellular signal regulated kinase 1/2,ERKl/2)表达的影响,为临床应用高压氧治疗TBI提供实验依据. 方法 SD大鼠24只,随机数字表法分为假手术组、TBI组和高压氧治疗组,每组8只.假手术组仅行开颅手术不致TBI; TBI组采用Alice打击法以50 g重锤自40 cm垂直高度自由落体打击制作大鼠急性TBI模型；高压氧组在TBI基础上行高压氧治疗(1次/d,连续10 d).各组大鼠于处理后14 d进行神经功能损伤评分(neurological severity scores,NSS).随后处死动物,获取脑组织,用RT - PCR方法测定脑组织内ERK1/2 mRNA的表达.免疫组化染色确定ERK表达的细胞定位,吸光度分析了解ERK蛋白变化. 结果与TBI组比较,高压氧治疗组14 d NSS评分明显降低(P＜0.01).同时,高压氧治疗组ERK1/2 mRNA和蛋白表达明显降低(P＜0.05). 结论高压氧治疗能明显降低急性TBI大鼠NSS评分及ERK1/2表达,提示高压氧治疗可能通过下调ERK1/2表达改善神经功能.
목적 탐토고압양치료대급성창상성뇌손상(traumatic brain injury,TBI)대서신경행위학급세포외신호조절격매(extra- cellular signal regulated kinase 1/2,ERKl/2)표체적영향,위림상응용고압양치료TBI제공실험의거. 방법 SD대서24지,수궤수자표법분위가수술조、TBI조화고압양치료조,매조8지.가수술조부행개로수술불치TBI; TBI조채용Alice타격법이50 g중추자40 cm수직고도자유락체타격제작대서급성TBI모형；고압양조재TBI기출상행고압양치료(1차/d,련속10 d).각조대서우처리후14 d진행신경공능손상평분(neurological severity scores,NSS).수후처사동물,획취뇌조직,용RT - PCR방법측정뇌조직내ERK1/2 mRNA적표체.면역조화염색학정ERK표체적세포정위,흡광도분석료해ERK단백변화. 결과 여TBI조비교,고압양치료조14 d NSS평분명현강저(P＜0.01).동시,고압양치료조ERK1/2 mRNA화단백표체명현강저(P＜0.05). 결론 고압양치료능명현강저급성TBI대서NSS평분급ERK1/2표체,제시고압양치료가능통과하조ERK1/2표체개선신경공능.
Objective To explore the effects of hyperbaric oxygen (HBO) treatment on expression of extra-cellular signal regulated kinase 1/2 (ERK 1/2) and neurological function in acute traumatic brain injury (TBI) rats so as to offer trial support for clinical application of HBO in TBI.Methods Twenty-four adult Sprague-Dawley rats were randomly divided into sham-operation group,TBI group and HBO treatment group,with eight rats per group.The sham-operation group was free from TBI but skull was opened only but the TBI group was subjected to TBI according to Alice method.TheHBO treatment group was treated with HBO for 10 days ( one time per day) after TBI operation.On day 14,NSS rating was performed in all rats.Then,the rats were sacrificed and cortex of which was obtained to measure the expression of ERK1/2 by using reverse transcription-polymerase chain reaction (RT-PCR).The localization of the expression of ERK was detected by immunohistochemical staining and the changes in ERK protein was analyzed by optical density.Results The NSS score in the HBO group was significantly decreased at day 14 after HBO treatment ( P ＜ 0.01 ) and the expression of ERK1/2 in HBO group was also significantly decreased (P ＜ 0.05 ) in comparison with the TBI group.Conclusions HBO treatment can significantly decrease NSS score and expression of ERK1/2 in acute TBI rats,indicating that HBO may improve neurological function through down-regulating expression of ERK1/2.