中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2008年
4期
500-501
,共2页
罗仪%李世文%沃飞%郑新民%胡礼泉%郑航%胡万里%陈婵
囉儀%李世文%沃飛%鄭新民%鬍禮泉%鄭航%鬍萬裏%陳嬋
라의%리세문%옥비%정신민%호례천%정항%호만리%진선
雌二醇%前列腺%平滑肌细胞%细胞周期
雌二醇%前列腺%平滑肌細胞%細胞週期
자이순%전렬선%평활기세포%세포주기
Estradiol%Prostate%Smooth muscle cells%Cell cycle
目的 观察17β-雌二醇(E2)对大鼠前列腺平滑肌细胞(PSMC)增殖的影响.方法 取体重(253±28)g的雄性SD大鼠30只,无菌切取前列腺,应用酶消化法行原代细胞培养.取3～4代传代细胞,分别加入不同浓度E2(0.1～100)nmol/L处理72 h,应用流式细胞仪检测细胞周期、细胞凋亡及其相关蛋白Cyclin D1,应用western blot法检测bcl-2和bax表达.结果 E2(1、10 nmol/L)促进PSMC从G1期向S期过渡,其S期细胞比率分别为(18.50±4.98)%、(21.16±4.83)%,显著高于对照组(12.39±2.64)%(P＜0.05),并伴随Cyclin D1蛋白表达显著增高.而高浓度E2(100 nmol/L)则抑制细胞增殖,其S期细胞比率为(7.98±1.92)%,显著低于对照组(P＜0.05),并伴随bax表达显著增加和细胞凋亡率显著升高.结论 低浓度E2能够上调CyclinD1表达加速G1期向S过渡从而促进大鼠PSMC增殖,高浓度E2则通过增加bax表达促进细胞凋亡.
目的 觀察17β-雌二醇(E2)對大鼠前列腺平滑肌細胞(PSMC)增殖的影響.方法 取體重(253±28)g的雄性SD大鼠30隻,無菌切取前列腺,應用酶消化法行原代細胞培養.取3～4代傳代細胞,分彆加入不同濃度E2(0.1～100)nmol/L處理72 h,應用流式細胞儀檢測細胞週期、細胞凋亡及其相關蛋白Cyclin D1,應用western blot法檢測bcl-2和bax錶達.結果 E2(1、10 nmol/L)促進PSMC從G1期嚮S期過渡,其S期細胞比率分彆為(18.50±4.98)%、(21.16±4.83)%,顯著高于對照組(12.39±2.64)%(P＜0.05),併伴隨Cyclin D1蛋白錶達顯著增高.而高濃度E2(100 nmol/L)則抑製細胞增殖,其S期細胞比率為(7.98±1.92)%,顯著低于對照組(P＜0.05),併伴隨bax錶達顯著增加和細胞凋亡率顯著升高.結論 低濃度E2能夠上調CyclinD1錶達加速G1期嚮S過渡從而促進大鼠PSMC增殖,高濃度E2則通過增加bax錶達促進細胞凋亡.
목적 관찰17β-자이순(E2)대대서전렬선평활기세포(PSMC)증식적영향.방법 취체중(253±28)g적웅성SD대서30지,무균절취전렬선,응용매소화법행원대세포배양.취3～4대전대세포,분별가입불동농도E2(0.1～100)nmol/L처리72 h,응용류식세포의검측세포주기、세포조망급기상관단백Cyclin D1,응용western blot법검측bcl-2화bax표체.결과 E2(1、10 nmol/L)촉진PSMC종G1기향S기과도,기S기세포비솔분별위(18.50±4.98)%、(21.16±4.83)%,현저고우대조조(12.39±2.64)%(P＜0.05),병반수Cyclin D1단백표체현저증고.이고농도E2(100 nmol/L)칙억제세포증식,기S기세포비솔위(7.98±1.92)%,현저저우대조조(P＜0.05),병반수bax표체현저증가화세포조망솔현저승고.결론 저농도E2능구상조CyclinD1표체가속G1기향S과도종이촉진대서PSMC증식,고농도E2칙통과증가bax표체촉진세포조망.
Objective To investigate the effect of 17β-estradiol(E2)on the proliferation of prostatic smooth muscle cells(PSMC).Methods The mature male rat PsMC(subcuhured for 3-4 passages)were obtained by enzyme-digesting and exposed to gradient concentrations(0.1-100 nmol/L)of E2,for 72 h.The progression of cell cycle,the apoptosis and the expression of Cyclin D1 were examined by flow cytometry.bcl-2 and bax proteins were detected by western blot.Results E2(1,10 nmol/L)promoted the PSMC proliferation by accelerating their cell cycle progression from G1 to S phases,The percentage of cells in S phases of the two group were(18.50±4.98)% and (21.16±4.83)%,respectively,significantly hisher than those in the control group(12.39±2.64)%(.P＜0.05),and the expression of CyclinD1 was siguificantly up-regulated.However,high concentration of E2(100 nmol/L)inhibitod the cell proliferation and percentage of cells in S phase was(7.98±1.92)%,significantly lower than in control group,and the expression of bax and apoptosis rate were significantly increased.Conclusion E2 can promote rat PSMC proliferation by enhancing the expression of CyclinD1 which accelerates G1 to S phase transition and induce apoptosis of the cells by up-regulating the expression of bax.
