中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2008年
6期
437-440
,共4页
黄正根%吴军%罗高兴%贺伟峰%甘成军%袁顺宗%贾雄飞%谭江琳%王晓娟%葛良鹏%魏泓
黃正根%吳軍%囉高興%賀偉峰%甘成軍%袁順宗%賈雄飛%譚江琳%王曉娟%葛良鵬%魏泓
황정근%오군%라고흥%하위봉%감성군%원순종%가웅비%담강림%왕효연%갈량붕%위홍
移植,异种%胚胎%皮肤前体组织%创面修复
移植,異種%胚胎%皮膚前體組織%創麵脩複
이식,이충%배태%피부전체조직%창면수복
Transplantation,heterologous%Embryo%Skin precursor tissue%Wound healing
目的 系统筛选猪胚胎皮肤前体组织异种移植的最佳妊娠时间窗,了解其创面修复能力. 方法 取胎龄35、42、56、70 d的胎猪皮肤前体组织,制成微粒后移植于BABL/c裸鼠背部创面,以整形患者术后剩余皮肤或者成年猪皮覆盖.观察术后移植物生长发育特性,并于移植后6、12周取材,用组织学方法观察其形态结构及成瘤性. 结果 胎猪皮肤前体组织移植后具有如下特点:(1)能成活并继续生长发育,微粒可融合成片;胎龄35、42、56、70 d的前体组织移植后12周,新生组织面积分别为(18±8)、(47±6)、(31±12)、(20±8)mm2,其中胎龄42 d的组织与其余三者比较,差异有统计学意义(P＜0.05).(2)新生(猪)皮肤组织具有表皮层和真皮层,真皮乳头明显.(3)能生长成为具有毛发、皮脂腺及汗腺等皮肤附属器的"完整"皮肤,且表皮层有黑索细胞.(4)胎龄56、70 d的胎猪皮肤前体组织移植后未出现畸胎瘤. 结论 胎龄56 d的胎猪皮肤前体组织可用于异种移植修复皮肤创面.
目的 繫統篩選豬胚胎皮膚前體組織異種移植的最佳妊娠時間窗,瞭解其創麵脩複能力. 方法 取胎齡35、42、56、70 d的胎豬皮膚前體組織,製成微粒後移植于BABL/c裸鼠揹部創麵,以整形患者術後剩餘皮膚或者成年豬皮覆蓋.觀察術後移植物生長髮育特性,併于移植後6、12週取材,用組織學方法觀察其形態結構及成瘤性. 結果 胎豬皮膚前體組織移植後具有如下特點:(1)能成活併繼續生長髮育,微粒可融閤成片;胎齡35、42、56、70 d的前體組織移植後12週,新生組織麵積分彆為(18±8)、(47±6)、(31±12)、(20±8)mm2,其中胎齡42 d的組織與其餘三者比較,差異有統計學意義(P＜0.05).(2)新生(豬)皮膚組織具有錶皮層和真皮層,真皮乳頭明顯.(3)能生長成為具有毛髮、皮脂腺及汗腺等皮膚附屬器的"完整"皮膚,且錶皮層有黑索細胞.(4)胎齡56、70 d的胎豬皮膚前體組織移植後未齣現畸胎瘤. 結論 胎齡56 d的胎豬皮膚前體組織可用于異種移植脩複皮膚創麵.
목적 계통사선저배태피부전체조직이충이식적최가임신시간창,료해기창면수복능력. 방법 취태령35、42、56、70 d적태저피부전체조직,제성미립후이식우BABL/c라서배부창면,이정형환자술후잉여피부혹자성년저피복개.관찰술후이식물생장발육특성,병우이식후6、12주취재,용조직학방법관찰기형태결구급성류성. 결과 태저피부전체조직이식후구유여하특점:(1)능성활병계속생장발육,미립가융합성편;태령35、42、56、70 d적전체조직이식후12주,신생조직면적분별위(18±8)、(47±6)、(31±12)、(20±8)mm2,기중태령42 d적조직여기여삼자비교,차이유통계학의의(P＜0.05).(2)신생(저)피부조직구유표피층화진피층,진피유두명현.(3)능생장성위구유모발、피지선급한선등피부부속기적"완정"피부,차표피층유흑색세포.(4)태령56、70 d적태저피부전체조직이식후미출현기태류. 결론 태령56 d적태저피부전체조직가용우이충이식수복피부창면.
Objective To select the optimal pregnancy time window of embryonic pig skin precursor tissue for xenotransplantation and study its ability in wound repair. Methods Skin precursor tissues were obtained from pig fetus of fetal age of 35,42,56,70 days,and were minced into microskin and transplanted to dorsal wounds of BABL/c nude mice.then they were covered with residual skin after plastic surgery of patients or adult pig skin(white).The characteristics of growth and development were observed after transplantation.Pathological examination was performed on 6 and 12 post operation weeks respectively to observe the tissue structure and tumorigenicity. Results Skin preeursor tissues from fetal pig survived and developed after transplantation,and the microskin fused.New tissue area from skin precursor tissues with fetal age of 42 days was(47±6)mm.,which was higher than that of35 days(18±8 mm2),56 days(31±12 mm2),70days(20±8 mm2,P＜0.05).The skin precursor developed into"intact skin"with hair,sebaceous glands and sweat glands.and melanocytes were also detected in epidermis.The newly-grown skin tissue included epidermal and dermal layer.and obvious dermal papillae.Terotoma was not found after transplantation in skin precursor tissue with fetal age of 56,70 days. Conclusion Fetal pig skin precursor tissue with fetal age of 56 days can be used to repair wound as xenotransplantion.
