CAJ | 학술논문

目的探讨阿魏酸钠在糖尿病大鼠阴茎勃起功能障碍中的干预作用及其机制.方法选择雄性SD大鼠通过腹腔内注射链脲佐菌素方法建立糖尿病大鼠模型44只,然后将成模大鼠分成糖尿病干预组22只和糖尿病对照组22只,并设正常对照组.其中糖尿病干预组每日灌胃法给予阿魏酸钠100 tag·kg-1·d-1干预.12周后观察大鼠阴茎勃起功能,并获取阴茎海绵体组织和血清中的超氧化物歧化酶(SOD),过氧化氢酶(CAT),一氧化氮合酶(NOS)与丙二醛(MDA)的水平变化和阴茎海绵体组织的病理学改变.结果成功建立糖尿病大鼠模型.与糖尿病对照组相比,糖尿病干预组和正常对照组大鼠阴茎勃起功能更好,3组的阴茎勃起率分别为22%、66%和100%.SOD、CAT和NOS在糖尿病对照组中的活性均低于正常对照组和糖尿病干预组;NO在正常对照组和糖尿病干预组的含量[(112±28)nmol/ml、(137±25)nmol/ml]高于糖尿病对照组[(56±10)nmol/ml,均P<0.01];MDA在正常对照组和糖尿病干预组的含量低于糖尿病对照组(均P<0.01).光镜下,糖尿病对照组阴茎海绵体平滑肌数量减少,间质血管管壁增厚,管腔变窄.结论阿魏酸钠可能通过调节自由基代谢、抗氧化损伤和促进NO生成增加的途径,对糖尿病性勃起功能障碍起到干预作用.
목적 탐토아위산납재당뇨병대서음경발기공능장애중적간예작용급기궤제.방법 선택웅성SD대서통과복강내주사련뇨좌균소방법건립당뇨병대서모형44지,연후장성모대서분성당뇨병간예조22지화당뇨병대조조22지,병설정상대조조.기중당뇨병간예조매일관위법급여아위산납100 tag·kg-1·d-1간예.12주후관찰대서음경발기공능,병획취음경해면체조직화혈청중적초양화물기화매(SOD),과양화경매(CAT),일양화담합매(NOS)여병이철(MDA)적수평변화화음경해면체조직적병이학개변.결과 성공건립당뇨병대서모형.여당뇨병대조조상비,당뇨병간예조화정상대조조대서음경발기공능경호,3조적음경발기솔분별위22%、66%화100%.SOD、CAT화NOS재당뇨병대조조중적활성균저우정상대조조화당뇨병간예조;NO재정상대조조화당뇨병간예조적함량[(112±28)nmol/ml、(137±25)nmol/ml]고우당뇨병대조조[(56±10)nmol/ml,균P<0.01];MDA재정상대조조화당뇨병간예조적함량저우당뇨병대조조(균P<0.01).광경하,당뇨병대조조음경해면체평활기수량감소,간질혈관관벽증후,관강변착.결론 아위산납가능통과조절자유기대사、항양화손상화촉진NO생성증가적도경,대당뇨병성발기공능장애기도간예작용.
Objective To investigate the effect and mechanism of sodium ferulate (SF) on reversing erectile dysfunction in diabetes mellitus (DM) rats. Methods Forty-four male adult Sprague-Dawley rats were induced for diabetes by an intraperitoneal injection of streptozotocin. Then the successful models were randomly divided into DM+SF group and DM group(22 rats each respectively). The rats in DM+SF group were treated with sodium ferulate (100 mg·kg-1·d-1) through a daily gastric lavage. At Week 12, the erectile function of all rats was evaluated and serum samples were harvested. The SOD, CAT, NOS, MDA and NO levels in corpus cavernosum and serum were all measured. The pathologic change in penile cavernous body was observed microscopically. Results. The diabetic rat models were successfully established. The erectile function was much better in normal control group and DM+SF group than that in DM group. And the penile erection rates in three groups were 100%, 66% and 22% respectively. The activity levels of SOD, CAT and NOS were markedly decreased in DM group as compared to those in normal control group and DM+SF group (P<0.01). The NO content was approximately equal in normal control group and DM+SF group(112+28)nmol/ml vs(137±25)nmol/ml. But both were much higher than that in DM group(56±10) nmol/ml, both P<0.01. The MDA content was markedly increased in DM group as compared to those in normal control group and DM+SF group (both P<0.01). Microscopically, muscle fibers in penile cavernous body arranged disorderly, muscular mantle damaged and desmoplasia scattered among muscle fibers in DM group. Conclusion Sodium ferulate may play interventional roles in reversing diabetic erectile dysfunction through metabolic regulation of free radicals, antagonism of oxidative insults and enhancement of NO production.