CAJ | 학술논문

采用酶联免疫吸附法(ELISA)，以两个陆地棉(Gossypium hirsutum L.)品种唐棉2号(抗病)、鄂荆1号(感病)为试材，人工接种，研究了棉花黄萎病菌(Verticillium dahliae)落叶型和非落叶型菌系致害对棉苗4种内源激素的动态变化。结果表明，病菌侵染棉苗后，落叶型菌系处理比非落叶型菌系处理的棉苗子叶中含有IAA、GA4和ABA的量要高，尤其是ABA含量，从接种后的3～5d开始直至第15d，落叶型菌系处理始终高于非落叶型菌系处理，在达到最高峰时，落叶型菌系处理唐棉2号与鄂荆1号棉苗子叶的ABA含量分别是546和726ng·g-1FW，相当于非落叶型菌系处理的1．3和4．2倍；感病品种的内源激素变化较抗病品种更加敏感。结果分析初步表明，病菌侵染后引起棉株ABA含量的显著增高是导致落叶的主要因素之一。
채용매련면역흡부법(ELISA)，이량개륙지면(Gossypium hirsutum L.)품충당면2호(항병)、악형1호(감병)위시재，인공접충，연구료면화황위병균(Verticillium dahliae)락협형화비락협형균계치해대면묘4충내원격소적동태변화。결과표명，병균침염면묘후，락협형균계처리비비락협형균계처리적면묘자협중함유IAA、GA4화ABA적량요고，우기시ABA함량，종접충후적3～5d개시직지제15d，락협형균계처리시종고우비락협형균계처리，재체도최고봉시，락협형균계처리당면2호여악형1호면묘자협적ABA함량분별시546화726ng·g-1FW，상당우비락협형균계처리적1．3화4．2배；감병품충적내원격소변화교항병품충경가민감。결과분석초보표명，병균침염후인기면주ABA함량적현저증고시도치락협적주요인소지일。
Using Enzyme-linked immunosorbentassays (ELISA), the dynamics of plant endoge-nous hormone on two cotton cultivars Tangmi-an2 and Ejing1 infected by defoliating and non-defoliating strains of Verticillium dahliae werestudied. The results were summarized as fol-lows: pathogen infection resulted in the changesof endogenous hormone of cotton cotyledon. Compared with the contents in cotyledon inocu-lated with nondefoliating strains, IAA, GA4 andABA contents in cotyledon inoculated with defo-liating strains were higher, especially the ABAcontent kept higher level from 3～5 days to 15days after inoculation. When the peak ap-pearred, the ABA contents of Tangmian2 andEjingl were 546 and 726ng · g 1Fw, respective-ly, which were 1. 3 and 4. 2 times more thanthose of two cultivars inoculated with nondefoli-ating strains. Susceptible variety was more sus-ceptible than resistant variety on the changes ofplant endogenous hormone. Based on the re-suits, it could be concluded that the rapid accu-mulation of ABA was one of main factors of de-foliation.