CAJ | 학술논문

利用同源克隆策略,从风信子中分离出一个MADS box基因,命名为HoMADS2.序列比较分析表明,HoMADS2与B类MADS box蛋白具有较高的同源性.分子进化树分析显示,HoMADS2与PI家族类聚在一起.同时,在HoMADS2的K box和C末端区域均具有PI家族的特征序列.以上序列分析结果表明,HoMADS2可能是PI的一个同源基因.RNA分子杂交结果显示,HoMADS2在四轮花器官中均表达,其表达模式不同于双子叶植物中PI同源基因.利用风信子离体花器官再生系统研究表明,HoMADS2在再生花芽中的表达不同于HoMADS1和HAG1,该基因在再生花芽发育过程中组成型表达,不受外源细胞分裂素和生长素的影响.
이용동원극륭책략,종풍신자중분리출일개MADS box기인,명명위HoMADS2.서렬비교분석표명,HoMADS2여B류MADS box단백구유교고적동원성.분자진화수분석현시,HoMADS2여PI가족류취재일기.동시,재HoMADS2적K box화C말단구역균구유PI가족적특정서렬.이상서렬분석결과표명,HoMADS2가능시PI적일개동원기인.RNA분자잡교결과현시,HoMADS2재사륜화기관중균표체,기표체모식불동우쌍자협식물중PI동원기인.이용풍신자리체화기관재생계통연구표명,HoMADS2재재생화아중적표체불동우HoMADS1화HAG1,해기인재재생화아발육과정중조성형표체,불수외원세포분렬소화생장소적영향.
A MADS box gene HoMADS2 was cloned from Hyacinthus orientalis L.in this study.Sequence comparison revealed that HoMADS2 was highly homologous to the class B MADS box genes.Furthermore,phylogenetic analysis showed that HoMADS2 was closely related to PI gene family and this was also supported by the presence of specific diagnostic sites of PI homologs in K box domain and C terminal region,suggesting that HoMADS2 might be a PI-like gene.HoMADS2 mRNA was accumulated in all floral organs,different from the expression patterns of PI homologs in dicots.During in vitro flower development,HoMADS2 expression was constitutively expressed and not affected by the presence of cytokinin and auxin in the regenerated flowers.Our results indicated that the expression of HoMADS2 is different from those of both HAG1 and HoMADS1 in responding to plant hormones during in vitro flower development.