复旦学报（医学版）
複旦學報（醫學版）
복단학보（의학판）
FUDAN UNIVERSITY JOURNAL OF MEDICAL SCIENCES
2009年
2期
142-148
,共7页
叶希韵%徐敏华%李晓峰%王耀发
葉希韻%徐敏華%李曉峰%王耀髮
협희운%서민화%리효봉%왕요발
山楂叶总黄酮%鹌鹑%血脂%脂肪肝%抗氧化作用
山楂葉總黃酮%鵪鶉%血脂%脂肪肝%抗氧化作用
산사협총황동%암순%혈지%지방간%항양화작용
hawthorn leaf flavonoids%quail%blood lipid%fatty liver%antioxidant capacity
目的 研究不同浓度的山楂叶总黄酮对鹌鹑血脂和脂肪肝形成的防治作用.方法 将60只雌、雄各半的鹌鹑分成6组:正常组(N)喂以普通饲料;高脂饮食模型组(M)喂以高胆固醇脂饲料,诱发形成脂肪肝模型;脂必妥药物对照组(ZBT)、山楂叶总黄酮低、中、高浓度实验组(HLF1、HLF2、HLF3)在喂高脂饲料的同时分别喂1 g/kg体重ZBT、20 mg/kg、40 mg/kg、80 mg/kg体重山楂叶总黄酮溶液各1 mL.实验6个月后取动物血清、肝脏,检测动物血清、肝脏组织中总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷丙转氨酶(AST)、谷草转氨酶(ALT)的含量,制备鹌鹑肝脏组织石蜡切片和电镜切片观察其病理学变化.结果 山楂叶总黄酮实验各组与模型组相比,其血清和肝脏匀浆液的TC、TG、LDL-C、MDA、AST、ALT等含量均显著下降(P<0.001),而HDL-C、GSH、SOD含量则明显上升(P<0.001),且山楂叶总黄酮的浓度呈明显的量效关系.石蜡切片和电镜切片也显示山楂叶总黄酮实验组动物肝脏中的脂质积累和脂肪肝病理学变化明显轻于模型组.结论 山楂叶总黄酮具有明显降低血脂、减轻动物肝脏内各类脂质沉积的作用,还具有很好的抗氧化作用,能显著地保护肝脏组织的生理生化功能,对由高脂饮食引起的高血脂和脂肪肝具有明显的防治作用.
目的 研究不同濃度的山楂葉總黃酮對鵪鶉血脂和脂肪肝形成的防治作用.方法 將60隻雌、雄各半的鵪鶉分成6組:正常組(N)餵以普通飼料;高脂飲食模型組(M)餵以高膽固醇脂飼料,誘髮形成脂肪肝模型;脂必妥藥物對照組(ZBT)、山楂葉總黃酮低、中、高濃度實驗組(HLF1、HLF2、HLF3)在餵高脂飼料的同時分彆餵1 g/kg體重ZBT、20 mg/kg、40 mg/kg、80 mg/kg體重山楂葉總黃酮溶液各1 mL.實驗6箇月後取動物血清、肝髒,檢測動物血清、肝髒組織中總膽固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、丙二醛(MDA)、超氧化物歧化酶(SOD)、穀胱甘肽(GSH)、穀丙轉氨酶(AST)、穀草轉氨酶(ALT)的含量,製備鵪鶉肝髒組織石蠟切片和電鏡切片觀察其病理學變化.結果 山楂葉總黃酮實驗各組與模型組相比,其血清和肝髒勻漿液的TC、TG、LDL-C、MDA、AST、ALT等含量均顯著下降(P<0.001),而HDL-C、GSH、SOD含量則明顯上升(P<0.001),且山楂葉總黃酮的濃度呈明顯的量效關繫.石蠟切片和電鏡切片也顯示山楂葉總黃酮實驗組動物肝髒中的脂質積纍和脂肪肝病理學變化明顯輕于模型組.結論 山楂葉總黃酮具有明顯降低血脂、減輕動物肝髒內各類脂質沉積的作用,還具有很好的抗氧化作用,能顯著地保護肝髒組織的生理生化功能,對由高脂飲食引起的高血脂和脂肪肝具有明顯的防治作用.
목적 연구불동농도적산사협총황동대암순혈지화지방간형성적방치작용.방법 장60지자、웅각반적암순분성6조:정상조(N)위이보통사료;고지음식모형조(M)위이고담고순지사료,유발형성지방간모형;지필타약물대조조(ZBT)、산사협총황동저、중、고농도실험조(HLF1、HLF2、HLF3)재위고지사료적동시분별위1 g/kg체중ZBT、20 mg/kg、40 mg/kg、80 mg/kg체중산사협총황동용액각1 mL.실험6개월후취동물혈청、간장,검측동물혈청、간장조직중총담고순(TC)、삼선감유(TG)、고밀도지단백(HDL-C)、저밀도지단백(LDL-C)、병이철(MDA)、초양화물기화매(SOD)、곡광감태(GSH)、곡병전안매(AST)、곡초전안매(ALT)적함량,제비암순간장조직석사절편화전경절편관찰기병이학변화.결과 산사협총황동실험각조여모형조상비,기혈청화간장균장액적TC、TG、LDL-C、MDA、AST、ALT등함량균현저하강(P<0.001),이HDL-C、GSH、SOD함량칙명현상승(P<0.001),차산사협총황동적농도정명현적량효관계.석사절편화전경절편야현시산사협총황동실험조동물간장중적지질적루화지방간병이학변화명현경우모형조.결론 산사협총황동구유명현강저혈지、감경동물간장내각류지질침적적작용,환구유흔호적항양화작용,능현저지보호간장조직적생리생화공능,대유고지음식인기적고혈지화지방간구유명현적방치작용.
Objective To investigate the effect of hawthorn leaf flavonoids (HLFs) with the different concentrations in prevention of high blood lipids and fatty liver in quails. Methods Sixty quails were divided into six groups:normal (N),high lipids food model (M),positive control (ZBT),HLF1,HLF2 and HLF3 groups.The animals in the N group were fed with normal food,while the M group was feed with high lipids food to establish the animal model of fatty liver.Besides high-fat diet,HLF1,HLF2 and HLF3 groups were also fed with 1 mL HLFs in the doses of 20 mg/kg,40 mg/kg and 80 mg/kg respectively.The ZBT group was fed with 1 g/kg of ZhiBiTuo and used as the positive control.After the experiments of six months,the serum and liver of the quails were taken and used to evaluate the contents of total cholesterol (TC),triglyceride (TG),high density lipid cholesterol(HDL-C),low density lipid cholesterol (LDL-C),superoxide dismutase (SOD),malondialdehyde (MDA),glutathione (GSH),alanine amino transferase (ALT) and glutamic oxaloacetic transaminase (AST).Meanwhile,the microscopic and electronic microscopic sections were used to observe the pathological changes of the livers of the quails. Results Compared with the M group,HLFs significantly lowered the content of TC,TG,LDL-C,MDA,AST and ALT in the serum and livers of the quails in other groups (P<0.001).However,the levels of HDL-C,SOD and GSH of the other groups were increased markedly (P<0.001).Moreover,the ratios of increase/decrease in the three HLF groups were depended on the concentrations of HLFs.Also,better liver structure and less lipid accumulation were also observed in the HLF groups when compared to the M group. Conclusions These results showed HLFs can lower blood lipid and cholesterol accumulation,improve the antioxidant capacity and protect the physiological function of liver.So,HLFs were effective in preventing and treating high blood lipids and fatty liver induced by high-fat diet.
